Search Results - "Willison, Keith R."

The substrate specificity of eukaryotic cytosolic chaperonin CCT by Willison, Keith R.

“…The cytosolic chaperonin CCT (chaperonin containing TCP-1) is an ATP-dependent double-ring protein machine mediating the folding of members of the eukaryotic…”
Get full text

The interaction network of the chaperonin CCT by Dekker, Carien, Stirling, Peter C, McCormack, Elizabeth A, Filmore, Heather, Paul, Angela, Brost, Renee L, Costanzo, Michael, Boone, Charles, Leroux, Michel R, Willison, Keith R

“…The eukaryotic cytosolic chaperonin containing TCP‐1 (CCT) has an important function in maintaining cellular homoeostasis by assisting the folding of many…”
Get full text

The crystal structure of yeast CCT reveals intrinsic asymmetry of eukaryotic cytosolic chaperonins by Dekker, Carien, Roe, S Mark, McCormack, Elizabeth A, Beuron, Fabienne, Pearl, Laurence H, Willison, Keith R

“…The cytosolic chaperonin CCT is a 1‐MDa protein‐folding machine essential for eukaryotic life. The CCT interactome shows involvement in folding and assembly of…”
Get full text

Equivalent Mutations in the Eight Subunits of the Chaperonin CCT Produce Dramatically Different Cellular and Gene Expression Phenotypes by Amit, Maya, Weisberg, Sarah J., Nadler-Holly, Michal, McCormack, Elizabeth A., Feldmesser, Ester, Kaganovich, Daniel, Willison, Keith R., Horovitz, Amnon

“…The eukaryotic cytoplasmic chaperonin-containing TCP-1 (CCT) is a complex formed by two back-to-back stacked hetero-octameric rings that assists the folding of…”
Get full text

Substantial CCT activity is required for cell cycle progression and cytoskeletal organization in mammalian cells by Grantham, Julie, Brackley, Karen I., Willison, Keith R.

“…The chaperonin CCT hexadecamer is required for the folding of non-native actins and tubulins in eukaryotic cells. Among the consequences of greatly reducing…”
Get full text

The malaria parasite chaperonin containing TCP-1 (CCT) complex: Data integration with other CCT proteomes by Wilkinson, Mark D, Ferreira, Josie L, Beeby, Morgan, Baum, Jake, Willison, Keith R

“…The multi-subunit chaperonin containing TCP-1 (CCT) is an essential molecular chaperone that functions in the folding of key cellular proteins. This paper…”
Get full text

Single cell quantification of microRNA from small numbers of non-invasively sampled primary human cells by Ho, Vanessa, Baker, Jonathan R., Willison, Keith R., Barnes, Peter J., Donnelly, Louise E., Klug, David R.

“…Expression levels of microRNAs (miRNAs) in single cells are low and conventional miRNA detection methods require amplification that can be complex,…”
Get full text

Structural Changes Underlying Allostery in Group II Chaperonins by Willison, Keith R.

“…The ATP-dependence of folding chamber closure in the 16-subunit homo-oligomeric chaperonin from archaea Methanococcus maripaludis (Mm-cpn) has been studied by…”
Get full text

Interactions of subunit CCT3 in the yeast chaperonin CCT/TRiC with Q/N-rich proteins revealed by high-throughput microscopy analysis by Nadler-Holly, Michal, Breker, Michal, Gruber, Ranit, Azia, Ariel, Gymrek, Melissa, Eisenstein, Miriam, Willison, Keith R, Schuldiner, Maya, Horovitz, Amnon

“…The eukaryotic chaperonin containing t-complex polypeptide 1 (CCT/TRiC) is an ATP-fueled machine that assists protein folding. It consists of two back-to-back…”
Get full text

Toward high-throughput oligomer detection and classification for early-stage aggregation of amyloidogenic protein by Tahirbegi, Bogachan, Magness, Alastair J, Piersimoni, Maria Elena, Teng, Xiangyu, Hooper, James, Guo, Yuan, Knöpfel, Thomas, Willison, Keith R, Klug, David R, Ying, Liming

“…Aggregation kinetics of proteins and peptides have been studied extensively due to their significance in many human diseases, including neurodegenerative…”
Get full text

A Two-step Mechanism for the Folding of Actin by the Yeast Cytosolic Chaperonin by Stuart, Sarah F., Leatherbarrow, Robin J., Willison, Keith R.

“…Actin requires the chaperonin containing TCP1 (CCT), a hexadecameric ATPase essential for cell viability in eukaryotes, to fold to its native state. Following…”
Get full text

Biological and Biomedical Applications of Two-Dimensional Vibrational Spectroscopy: Proteomics, Imaging, and Structural Analysis by Fournier, Frederic, Guo, Rui, Gardner, Elizabeth M, Donaldson, Paul M, Loeffeld, Christian, Gould, Ian R, Willison, Keith R, Klug, David R

“…In the last 10 years, several forms of two-dimensional infrared (2DIR) spectroscopy have been developed, such as IR pump−probe spectroscopy and photon-echo…”
Get full text

Structure and function of a protein folding machine: the eukaryotic cytosolic chaperonin CCT by Valpuesta, José M., Martı́n-Benito, Jaime, Gómez-Puertas, Paulino, Carrascosa, José L., Willison, Keith R.

“…Chaperonins are large oligomers made up of two superimposed rings, each enclosing a cavity used for the folding of other proteins. Among the chaperonins, the…”
Get full text

Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin by Altschuler, Gabriel M, Willison, Keith R

“…to a less stable but now productive folding intermediate I…”
Get full text

Sequential ATP-induced allosteric transitions of the cytoplasmic chaperonin containing TCP-1 revealed by EM analysis by Horovitz, Amnon, Rivenzon-Segal, Dalia, Wolf, Sharon G, Shimon, Liat, Willison, Keith R

“…The eukaryotic cytoplasmic chaperonin containing TCP-1 (CCT) is a hetero-oligomeric complex that assists the folding of actins, tubulins and other proteins in…”
Get full text

Protein identification and quantification by two-dimensional infrared spectroscopy: Implications for an all-optical proteomic platform by Fournier, Frédéric, Gardner, Elizabeth M, Kedra, Darek A, Donaldson, Paul M, Guo, Rui, Butcher, Sarah A, Gould, Ian R, Willison, Keith R, Klug, David R

“…Electron-vibration-vibration two-dimensional coherent spectroscopy, a variant of 2DIR, is shown to be a useful tool to differentiate a set of 10 proteins based…”
Get full text

Nanoscale tweezers for single-cell biopsies by Nadappuram, Binoy Paulose, Cadinu, Paolo, Barik, Avijit, Ainscough, Alexander J., Devine, Michael J., Kang, Minkyung, Gonzalez-Garcia, Jorge, Kittler, Josef T., Willison, Keith R., Vilar, Ramon, Actis, Paolo, Wojciak-Stothard, Beata, Oh, Sang-Hyun, Ivanov, Aleksandar P., Edel, Joshua B.

“…Much of the functionality of multicellular systems arises from the spatial organization and dynamic behaviours within and between cells. Current single-cell…”
Get full text

Acetylation Rather than H50Q Mutation Impacts the Kinetics of Cu(II) Binding to α‐Synuclein by Teng, Xiangyu, Sheveleva, Alena, Tuna, Floriana, Willison, Keith R., Ying, Liming

“…The interaction between α‐synuclein (αSyn) and Cu2+ has been suggested to be closely linked to brain copper homeostasis. Disruption of copper levels could…”
Get full text

Acetylation Rather than H50Q Mutation Impacts the Kinetics of Cu(II) Binding to α‐Synuclein by Teng, Xiangyu, Sheveleva, Alena, Tuna, Floriana, Willison, Keith R., Ying, Liming

“…The front cover artwork is provided by the group of Liming Ying at Imperial College London. The image shows that N‐terminal acetylation of α‐synuclein shifts…”
Get full text

Interplay between Copper, Phosphatidylserine, and α‑Synuclein Suggests a Link between Copper Homeostasis and Synaptic Vesicle Cycling by Teng, Xiangyu, Stefaniak, Ewelina, Willison, Keith R., Ying, Liming

“…Copper homeostasis is critical to the functioning of the brain, and its breakdown is linked with many brain diseases. Copper is also known to interact with the…”
Get full text