Characterization of a porcine model of post-operative pain
Background Management of acute pain related to surgical intervention, termed post‐operative pain or POP, continues to be a major healthcare challenge. While the rat plantar incision model provides valuable data to researchers about the mechanisms mediating POP, the development of topical and localiz...
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| Published in: | European journal of pain Vol. 18; no. 4; pp. 496 - 505 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Blackwell Publishing Ltd
01-04-2014
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Background
Management of acute pain related to surgical intervention, termed post‐operative pain or POP, continues to be a major healthcare challenge. While the rat plantar incision model provides valuable data to researchers about the mechanisms mediating POP, the development of topical and localized treatments in small animal models is limited. To help address these issues, we describe here the characterization of a large animal model of incisional pain.
Methods
Pigs underwent full‐skin incision or full‐skin and muscle incision and retraction (SMIR). Withdrawal thresholds were determined using the Von Frey test at baseline, 0.5–12 h post‐surgery and on days 1, 2, 3, 5 and 7 post‐surgery. The analgesic effects of systemic morphine [0.1 or 1.0 mg/kg intramuscular (i.m.) dose] and local anaesthetic ropivacaine were studied. Spontaneous pain‐like behaviours were scored and analysed. The effects on wound healing were evaluated by gross observation and by histopathological examination.
Results
Pigs incurring SMIR demonstrated significantly increased mechanical hypersensitivity compared with pigs that underwent full‐skin incision only (p < 0.05). Maximal analgesia was achieved with morphine (1 mg/kg i.m. dose) at 0.5 h post‐treatment. Local treatment with ropivacaine was effective at increasing the withdrawal threshold to Von Frey filaments compared with saline control (p < 0.05) for a period of at least 6 h. Wounds healed normally with no signs of infection, redness or swelling.
Conclusions
We propose that the pig model of incisional pain can provide an appropriate translational model for validating new topical and localized treatments for POP in humans. |
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| Bibliography: | ark:/67375/WNG-L2D010GN-3 istex:00213E5D255DE2461A5F7C0BC8D06787309A123F MD Biosciences and Lahav Research Institute ArticleID:EJP399 Conflicts of interest Funding sources This work has been funded by MD Biosciences and Lahav Research Institute. None declared. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1090-3801 1532-2149 |
| DOI: | 10.1002/j.1532-2149.2013.00399.x |