New technologies for DNA analysis – a review of the READNA Project

•Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of enrichment technologies.•Manipulating, visualizing and interrogating individual DNA molecules in nanosystems.•Nanopore Sequencing – first com...

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Published in:	New biotechnology Vol. 33; no. 3; pp. 311 - 330
Main Authors:	McGinn, Steven, Bauer, David, Brefort, Thomas, Dong, Liqin, El-Sagheer, Afaf, Elsharawy, Abdou, Evans, Geraint, Falk-Sörqvist, Elin, Forster, Michael, Fredriksson, Simon, Freeman, Peter, Freitag, Camilla, Fritzsche, Joachim, Gibson, Spencer, Gullberg, Mats, Gut, Marta, Heath, Simon, Heath-Brun, Isabelle, Heron, Andrew J., Hohlbein, Johannes, Ke, Rongqin, Lancaster, Owen, Le Reste, Ludovic, Maglia, Giovanni, Marie, Rodolphe, Mauger, Florence, Mertes, Florian, Mignardi, Marco, Moens, Lotte, Oostmeijer, Jelle, Out, Ruud, Pedersen, Jonas Nyvold, Persson, Fredrik, Picaud, Vincent, Rotem, Dvir, Schracke, Nadine, Sengenes, Jennifer, Stähler, Peer F., Stade, Björn, Stoddart, David, Teng, Xia, Veal, Colin D., Zahra, Nathalie, Bayley, Hagan, Beier, Markus, Brown, Tom, Dekker, Cees, Ekström, Björn, Flyvbjerg, Henrik, Franke, Andre, Guenther, Simone, Kapanidis, Achillefs N., Kaye, Jane, Kristensen, Anders, Lehrach, Hans, Mangion, Jonathan, Sauer, Sascha, Schyns, Emile, Tost, Jörg, van Helvoort, Joop M.L.M., van der Zaag, Pieter J., Tegenfeldt, Jonas O., Brookes, Anthony J., Mir, Kalim, Nilsson, Mats, Willcocks, James P., Gut, Ivo G.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 25-05-2016 Elsevier
Subjects:	Animals Annan fysik Biochemistry and Molecular Biology Biofysica Biofysik Biokemi och molekylärbiologi Biomedical Laboratory Science/Technology Biomedicinsk laboratorievetenskap/teknologi Biophysics Biotechnology - methods Click Chemistry Computer Science Computerized Image Processing Datoriserad bildbehandling DNA - analysis DNA - genetics EPS Exome - genetics Fysik Humans Laboratorium voor Biofysica Life Sciences Mass Spectrometry Medical and Health Sciences Medical Biotechnology Medicin och hälsovetenskap Medicinsk bioteknologi Natural Sciences Naturvetenskap Other Physics Topics Physical Sciences Quantitative Methods Sequence Analysis, DNA Signal and Image Processing VLAG signal processing, bioinformatics, statistical analysis, Nucleic Acid analysis, classification
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Summary:	•Discovery of ‘TUF’ DNA – unusually difficult to denature stretches of genomic DNA sequence.•Enrichment methods – new methods, benchmarking and descriptors of enrichment technologies.•Manipulating, visualizing and interrogating individual DNA molecules in nanosystems.•Nanopore Sequencing – first commercial nanopore sequencer from Oxford Nanopore Technologies. The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3rd and 4th generation of sequencing methods with nanopores and in situ sequencing, respectively.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1871-6784 1876-4347 1876-4347
DOI:	10.1016/j.nbt.2015.10.003