The influence of very small doses of alpha radiation on the stability of erythrocytes

The influence of very small doses of alpha radiation on the stability of erythrocytes

Our aim was to study the influence of low doses (0.2–4 μGy) of α radiation on the stability of human erythrocytes isolated from healthy and diabetic erythrocytes. Absorption spectroscopy was used to measure the level of red blood cell (RBC) hemolysis, along with Mössbauer spectroscopy, which is a hi...

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Published in:Microscopy research and technique Vol. 80; no. 1; pp. 131 - 143
Main Authors: Kaczmarska, Magdalena, Żydek, Dominika, Wilkłacz‐Potoczny, Justyna, Fornal, Maria, Grodzicki, Tomasz, Kochowska, Elżbieta, Kozak, Krzysztof, Gocal, Łukasz, Pohorecki, Władysław, Matlak, Krzysztof, Korecki, Józef, Burda, Květoslava
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-01-2017
Subjects:
Alpha Particles
Atomic force microscopy
Cells, Cultured
Dose-Response Relationship, Radiation
Erythrocyte Membrane - radiation effects
Erythrocytes
Erythrocytes - radiation effects
Gamma Rays
Hemoglobin
Hemoglobins - metabolism
Hemolysis
Hemolysis - radiation effects
Homeostasis - drug effects
Humans
Irradiation
membrane skeleton
Membranes
Microscopy, Atomic Force
Mössbauer spectroscopy
Networks
red blood cell
Red blood cells
α‐radiation
α-radiation
atomic force microscopy
Mössbauer spectroscopy
membrane skeleton
red blood cell
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Summary:Our aim was to study the influence of low doses (0.2–4 μGy) of α radiation on the stability of human erythrocytes isolated from healthy and diabetic erythrocytes. Absorption spectroscopy was used to measure the level of red blood cell (RBC) hemolysis, along with Mössbauer spectroscopy, which is a highly specific method suited to monitoring various hemoglobin forms. States of hemoglobin are sensitive to a homeostatic imbalance in red blood cells. Changes in the membrane skeleton organization of irradiated erythrocytes isolated from healthy donors were studied using atomic force microscopy (AFM). Hemolysis, in healthy red blood cells, showed characteristic discontinuities, depending on the α particle flux and the exposure time to the low doses applied. This phenomenon was not observed in severe diabetic cases, which could be a result of modified protein–lipid–sugar complexes and the attenuation/absence of some antioxidative enzymatic processes in their RBC membranes. Similar effects were also observed for red blood cells treated with low doses of neutron and γ‐radiation. AFM measurements demonstrated a reorganization of the RBC membrane skeleton network depending on the time of RBC exposure to α radiation. This suggests that the changes in the activity of the acute defense processes against free radicals which are activated within the erythrocyte membrane irradiated with α‐particles could additionally be up‐ or down regulated by modifications to the membrane–skeleton network. However, even the highest dose of α radiation applied in these studies did not cause any significant changes in the ability of hemoglobin to transport oxygen. Microsc. Res. Tech. 80:131–143, 2017. © 2016 Wiley Periodicals, Inc.
Bibliography:Review Editor: Prof. Jose Luis Toca‐Herrera
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ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22803