Best practices for germ-free derivation and gnotobiotic zebrafish husbandry

Best practices for germ-free derivation and gnotobiotic zebrafish husbandry

All animals are ecosystems with resident microbial communities, referred to as microbiota, which play profound roles in host development, physiology, and evolution. Enabled by new DNA sequencing technologies, there is a burgeoning interest in animal-microbiota interactions, but dissecting the specif...

Full description

Saved in:
Bibliographic Details
Published in:Methods in cell biology Vol. 138; pp. 61 - 100
Main Authors: Melancon, E, Gomez De La Torre Canny, S, Sichel, S, Kelly, M, Wiles, T J, Rawls, J F, Eisen, J S, Guillemin, K
Format: Journal Article
Language:English
Published: United States 2017
Subjects:
Animals
Biological Evolution
Germ-Free Life
Mammals - microbiology
Microbiota - genetics
Zebrafish - growth & development
Zebrafish - microbiology
Germ-free
Gnotobiotic
Microbiota
Husbandry
Sterile
Zebrafish
Bacterial colonization
Axenic
Microbiome
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:All animals are ecosystems with resident microbial communities, referred to as microbiota, which play profound roles in host development, physiology, and evolution. Enabled by new DNA sequencing technologies, there is a burgeoning interest in animal-microbiota interactions, but dissecting the specific impacts of microbes on their hosts is experimentally challenging. Gnotobiology, the study of biological systems in which all members are known, enables precise experimental analysis of the necessity and sufficiency of microbes in animal biology by deriving animals germ-free (GF) and inoculating them with defined microbial lineages. Mammalian host models have long dominated gnotobiology, but we have recently adapted gnotobiotic approaches to the zebrafish (Danio rerio), an important aquatic model. Zebrafish offer several experimental attributes that enable rapid, large-scale gnotobiotic experimentation with high replication rates and exquisite optical resolution. Here we describe detailed protocols for three procedures that form the foundation of zebrafish gnotobiology: derivation of GF embryos, microbial association of GF animals, and long-term, GF husbandry. Our aim is to provide sufficient guidance in zebrafish gnotobiotic methodology to expand and enrich this exciting field of research.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-2
ISSN:0091-679X
DOI:10.1016/bs.mcb.2016.11.005