Search Results - "Wiersma, Erik J."

How Sustainable are Different Levels of Consciousness? by Wiersma, Erik J.

“…The human brain processes a wide variety of inputs and does so either consciously or subconsciously. According to the global workspace theory, conscious…”
Get full text

Structural and Functional Analysis of J Chain-Deficient IgM by Wiersma, Erik J, Collins, Cathy, Fazel, Shafie, Shulman, Marc J

“…Previous studies have discerned two forms of polymeric mouse IgM: moderately cytolytic (complement-activating) pentamer, which contains J chain, and highly…”
Get full text

Analysis of the expressed heavy chain variable-region genes of Macaca fascicularis and isolation of monoclonal antibodies specific for the Ebola virus' soluble glycoprotein by Druar, Chris, Saini, Surinder S, Cossitt, Meredith A, Yu, Fei, Qiu, Xiangguo, Geisbert, Thomas W, Jones, Steven, Jahrling, Peter B, Stewart, Donald I. H, Wiersma, Erik J

“…The cynomolgus macaque, Macaca fascicularis, is frequently used in immunological and other biomedical research as a model for man; understanding it's antibody…”
Get full text

On the influence of vector design on antibody phage display by Soltes, Glenn, Hust, Michael, Ng, Kitty K.Y., Bansal, Aasthaa, Field, Johnathan, Stewart, Donald I.H., Dübel, Stefan, Cha, Sanghoon, Wiersma, Erik J.

“…Phage display technology is an established technology particularly useful for the generation of monoclonal antibodies (mAbs). The isolation of phagemid-encoded…”
Get full text

Epitope specificity and isotype of monoclonal anti-D antibodies dictate their ability to inhibit phagocytosis of opsonized platelets by Kjaersgaard, Mimi, Aslam, Rukhsana, Kim, Michael, Speck, Edwin R., Freedman, John, Stewart, Donald I.H., Wiersma, Erik J., Semple, John W.

“…Rh immune globulin (WinRho SDF; Cangene, Mississauga, ON, Canada) is an effective treatment for autoimmune thrombocytopenic purpura; however, maintaining a…”
Get full text

A new helper phage and phagemid vector system improves viral display of antibody Fab fragments and avoids propagation of insert-less virions by Soltes, Glenn, Barker, Heather, Marmai, Kristine, Pun, Elaine, Yuen, Amy, Wiersma, Erik J.

“…Phage display technology (PDT) is a powerful method for isolating functional gene products such as antigen-specific monoclonal antibodies (mAbs). To improve…”
Get full text

Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents by Druar, Chris, Yu, Fei, Barnes, Jodie L, Okinaka, Richard T, Chantratita, Narisara, Beg, Steve, Stratilo, Chad W, Olive, Andrew J, Soltes, Glenn, Russell, Michelle L, Limmathurotsakul, Direk, Norton, Robert E, Ni, Sally X, Picking, William D, Jackson, Paul J, Stewart, Don I.H, Tsvetnitsky, Vadim, Picking, Wendy L, Cherwonogrodzky, John W, Ketheesan, Natkunam, Peacock, Sharon J, Wiersma, Erik J

“…Burkholderia pseudomallei is a biothreat agent and an important natural pathogen, causing melioidosis in humans and animals. A type III secretion system…”
Get full text

Interplay of J chain and disulfide bonding in assembly of polymeric IgM by Fazel, S, Wiersma, E J, Shulman, M J

“…Normal mouse IgM is synthesized as hexamers in the absence of J chain and as pentamers in its presence. Previous work has suggested that polymer size is also…”
Get full text

Role of the Intronic Elements in the Endogenous Immunoglobulin Heavy Chain Locus by Wiersma, Erik J., Ronai, Diana, Berru, Maribel, Tsui, Florence W.L., Shulman, Marc J.

“…High level expression in mice of transgenes derived from the immunoglobulin heavy chain (IgH) locus requires both the core enhancer (Eμ) and the matrix…”
Get full text

How sustainable are different levels of consciousness? by Wiersma, Erik J

“…The human brain processes a wide variety of inputs and does so either consciously or subconsciously. According to the Global Workspace theory, conscious…”
Get full text

Corrigendum to “A new helper phage and phagemid vector system improves viral display of antibody Fab fragments and avoids propagation of insert-less virions” [Journal of Immunological Methods 274 (2003) 233–244] by Soltes, Glenn, Barker, Heather, Marmai, Kristine, Pun, Elaine, Yuen, Amy, Wiersma, Erik J.

Get full text

In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody by HEYMAN, B, WIERSMA, E. J, KINOSHITA, T

“…BALB/c mice were injected intravenously with three different monoclonal antibodies (mAbs) specific for complement receptor 1 (CR1). Two of the mAbs…”
Get full text

Role of the intronic elements in the endogenous immunoglobulin heavy chain locus. Either the matrix attachment regions or the core enhancer is sufficient to maintain expression by Wiersma, E J, Ronai, D, Berru, M, Tsui, F W, Shulman, M J

“…High level expression in mice of transgenes derived from the immunoglobulin heavy chain (IgH) locus requires both the core enhancer (Emu) and the matrix…”
Get full text

Assembly of IgM. Role of disulfide bonding and noncovalent interactions by Wiersma, EJ, Shulman, MJ

“…Polymeric IgM is usually envisaged as an array of mu 2L2 monomers in which the mu heavy chains are held together by disulfide bonds involving cysteines at…”
Get full text

Epitope mapping of four monoclonal antibodies specific for the human RhD antigen by Nickerson, Lise, Wiersma, Erik J

“…RhD is a highly immunogenic erythrocyte membrane protein, implicated in hemolytic disease of the newborn and other hemolytic disorders. Anti-RhD antibodies are…”
Get full text

Inhibition of immunological memory and T-independent humoral responses by monoclonal antibodies specific for murine complement receptors by Wiersma, E J, Kinoshita, T, Heyman, B

“…The importance of the complement system for mounting an antibody response in vivo was investigated by down-regulating and blocking the complement receptors…”
Get more information

Analysis of IgM structures involved in J chain incorporation by Wiersma, EJ, Chen, F, Bazin, R, Collins, C, Painter, RH, Lemieux, R, Shulman, MJ

“…J chain is associated with pentameric IgM and polymeric IgA. In IgM, J chain is disulfide bonded to the C575 residue of the mu-chain, located in the mu tail…”
Get full text

Evidence of IgG-mediated enhancement of the antibody response in vivo without complement activation via the classical pathway by Wiersma, E J, Nose, M, Heyman, B

“…The complement (C) dependency of IgG-mediated enhancement of the antibody response was investigated by immunizing mice with trinitrophenyl-coupled keyhole…”
Get more information