Search Results - "Wickner, William T."

A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly by Baker, Richard W., Jeffrey, Philip D., Zick, Michael, Phillips, Ben P., Wickner, William T., Hughson, Frederick M.

“…Fusion of intracellular transport vesicles requires soluble N-ethylmaleimide–sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18-family (SM)…”
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A distinct tethering step is vital for vacuole membrane fusion by Zick, Michael, Wickner, William T

“…Past experiments with reconstituted proteoliposomes, employing assays that infer membrane fusion from fluorescent lipid dequenching, have suggested that…”
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HOPS recognizes each SNARE, assembling ternary trans -complexes for rapid fusion upon engagement with the 4th SNARE by Song, Hongki, Orr, Amy S, Lee, Miriam, Harner, Max E, Wickner, William T

“…Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four…”
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Sec17 can trigger fusion of trans-SNARE paired membranes without Sec18 by Zick, Michael, Orr, Amy, Schwartz, Matthew L, Merz, Alexey J, Wickner, William T

“…Sec17 [soluble N -ethylmaleimide–sensitive factor (NSF) attachment protein; α-SNAP] and Sec18 (NSF) perform ATP-dependent disassembly of cis -SNARE complexes,…”
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Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion by Zick, Michael, Stroupe, Christopher, Orr, Amy, Douville, Deborah, Wickner, William T

“…Like other intracellular fusion events, the homotypic fusion of yeast vacuoles requires a Rab GTPase, a large Rab effector complex, SNARE proteins which can…”
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Profile of Thomas Südhof, James Rothman, and Randy Schekman, 2013 Nobel Laureates in Physiology or Medicine by Wickner, William T.

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Sec17/Sec18 can support membrane fusion without help from completion of SNARE zippering by Song, Hongki, Torng, Thomas L, Orr, Amy S, Brunger, Axel T, Wickner, William T

“…Membrane fusion requires R-, Qa-, Qb-, and Qc-family SNAREs that zipper into RQaQbQc coiled coils, driven by the sequestration of apolar amino acids. Zippering…”
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lipid-anchored SNARE supports membrane fusion by Xu, Hao, Zick, Michael, Wickner, William T, Jun, Youngsoo

“…Intracellular membrane fusion requires R-SNAREs and Q-SNAREs to assemble into a four-helical parallel coiled-coil, with their hydrophobic anchors spanning the…”
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N-terminal domain of vacuolar SNARE Vam7p promotes trans-SNARE complex assembly by Xu, Hao, Wickner, William T

“…SNARE-dependent membrane fusion in eukaryotic cells requires that the heptad-repeat SNARE domains from R- and Q-SNAREs, anchored to apposed membranes, assemble…”
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trans-SNARE complex assembly and yeast vacuole membrane fusion by Collins, Kevin M, Wickner, William T

“…cis-SNARE complexes (anchored in one membrane) are disassembled by Sec17p (α-SNAP) and Sec18p (NSF), permitting the unpaired SNAREs to assemble in trans. We…”
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Eugene Patrick Kennedy, 1919—2011 by Wickner, William T.

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Enolase Activates Homotypic Vacuole Fusion and Protein Transport to the Vacuole in Yeast by Decker, Bridget L., Wickner, William T.

“…Membrane fusion and protein trafficking to the vacuole are complex processes involving many proteins and lipids. Cytosol from Saccharomyces cerevisiae contains…”
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A Ypt/Rab Effector Complex Containing the Sec1 Homolog Vps33p Is Required for Homotypic Vacuole Fusion by Seals, Darren F., Eitzen, Gary, Margolis, Nathan, Wickner, William T., Price, Albert

“…Yeast vacuoles undergo priming, docking, and homotypic fusion, although little has been known of the connections between these reactions. Vacuole-associated…”
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Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion by Collins, Kevin M, Thorngren, Naomi L, Fratti, Rutilio A, Wickner, William T

“…SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole…”
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Trans-SNARE Interactions Elicit Ca2+Efflux from the Yeast Vacuole Lumen by Merz, Alexey J., Wickner, William T.

“…Ca2+transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca2+. Here, we…”
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Chris Raetz, scientist and enduring friend by Wickner, William T., Stubbe, JoAnne, Hirschberg, Carlos B., Garrett, Teresa, Dowhan, William

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Resolution of Organelle Docking and Fusion Kinetics in a Cell-Free Assay by Merz, Alexey J., Wickner, William T.

“…In vitro assays of compartment mixing have been key tools in the biochemical dissection of organelle docking and fusion. Many such assays measure compartment…”
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Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS by Song, Hongki, Wickner, William T

“…Yeast vacuolar membrane fusion has been reconstituted with R, Qa, Qb, and Qc-family SNAREs, Sec17/αSNAP, Sec18/NSF, and the hexameric HOPS complex. HOPS…”
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Correction: Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion by Zick, Michael, Stroupe, Christopher, Orr, Amy, Douville, Deborah, Wickner, William T

“…Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion. eLife 3:e01879. doi: 10.7554/eLife.01879…”
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Christian Raetz: scientist and friend extraordinaire by Dowhan, William, Nikaido, Hiroshi, Stubbe, JoAnne, Kozarich, John W, Wickner, William T, Russell, David W, Garrett, Teresa A, Brozek, Kathryn, Modrich, Paul

“…Chris Raetz passed away on August 16, 2011, still at the height of his productive years. His seminal contributions to biomedical research were in the genetics,…”
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