Short- and long-term effect of colorectal cancer targeting peptides conjugated to gold nanoparticles in rats’ liver and colon after single exposure

Peptides play important roles in the diagnosis, prognostic predictors, and treatment of various kinds of cancer. Peptides (p.C, p.L and p.14), derived from the phage display peptide libraries, specifically binds to colorectal cancer (CRC) cells in vitro. To allow tumor specificity and selectivity fo...

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Bibliographic Details
Published in:	Toxicological research (Seoul) Vol. 38; no. 3; pp. 259 - 273
Main Authors:	Adewale, Olusola B., Cairncross, Lynn, Xakaza, Hlumisa, Wickens, Nicolas, Anadozie, Scholastica O., Davids, Hajierah, Roux, Saartjie
Format:	Journal Article
Language:	English
Published:	Singapore Springer Nature Singapore 01-07-2022 한국독성학회
Subjects:	Biomedical and Life Sciences Biomedicine Original Original Article Pharmacology/Toxicology 예방의학 Diagnosis Peptides Polyethylene glycol Gold nanoparticles Colorectal cancer
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Summary:	Peptides play important roles in the diagnosis, prognostic predictors, and treatment of various kinds of cancer. Peptides (p.C, p.L and p.14), derived from the phage display peptide libraries, specifically binds to colorectal cancer (CRC) cells in vitro. To allow tumor specificity and selectivity for in vivo diagnosis of CRC, biotinylated p.C, p.L and p.14 were conjugated to AuNPs (14 nm) via the biotin-streptavidin interaction. Male Wistar rats were intravenously injected with a single dose (100 µg/kg body weight) of AuNPs (citrate-AuNPs, PEG-AuNPs, p.C-PEG-, p.L-PEG- and p.14-PEG-AuNPs). Animals were monitored for behavioral changes, and sacrificed either 14 days or 84 days post-injection. Biochemical changes, oxidative stress, and histology of the liver and colon were assessed. No significant changes were noted in the rats injected with all the AuNPs, except p.L-PEG-AuNPs that caused significant toxicity ( p < 0.05) 14 days post-exposure when compared to control group, as evidenced by increased relative liver weight, increased malondialdehyde levels and histological changes in the liver. These changes, however, returned to normalcy 84 days post-injection. It can be concluded, based on these findings, that p.L induced a transient toxicity in rats after a single intravenous injection, and can therefore be considered non-toxic long-term after a single exposure.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1976-8257 2234-2753
DOI:	10.1007/s43188-021-00108-y