Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes

Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes

Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent p...

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 24; no. 20; p. 15073
Main Authors: Kim, Young Ju, Jang, Mina, Roh, Jongtae, Lee, Yoon Jeong, Moon, Hee Jung, Byun, Jimin, Wi, Jihyun, Ko, Sung-Kyun, Tae, Jinsung
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-10-2023
MDPI
Subjects:
acidic pH
Disease
Ethylenediaminetetraacetic acid
Fluorescence
fluorescent imaging
fluorescent probe
Hydrogen-ion concentration
hydroxamate
Localization
Methylene blue
rhodamine B
Sensors
Signal transduction
Spectrum analysis
United Kingdom
South Korea
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Summary:Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been of great interest due to their ON/OFF fluorescence depending on the spirolactam ring’s opening/closure. By introducing the N-alkyl-hydroxamic acid instead of the alkyl amines in the spirolactam of rhodamine, we were able to tune the pH range where the ring opening and closing of the spirolactam occurs. This six-membered cyclic hydroxamate spirolactam ring of rhodamine B proved to be highly fluorescent in acidic pH environments. In addition, we could monitor pH changes of lysosomes in live cells and zebrafish.
Bibliography:These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242015073