Comparative efficacy and safety of anti-cryptosporidial agents: an in vitro study on nitazoxanide, halofuginone lactate, KDU731, and paromomycin against Cryptosporidium parvum

This study evaluated the effectiveness of anti-cryptosporidial agents nitazoxanide, halofuginone, the pyrazolopyridine analog KDU731, and paromomycin (PMC) in combating the significant zoonotic pathogen . The study utilized HCT-8 host cells to culture and fluorescent microscopy/quantitative PCR (qPC...

Full description

Saved in:

Bibliographic Details
Published in:	Frontiers in microbiology Vol. 15; p. 1463457
Main Authors:	Whitta, Saffron T G, Lamont, Bridget, Suwanarusk, Rossarin, Russell, Bruce M, Muhsin-Sharafaldine, Morad-Rémy
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 04-10-2024
Subjects:	anti-parasitics apicomplexa pathogen cryptosporidiosis Cryptosporidium halofuginone lactate nitazoxanide Cryptosporidium apicomplexa pathogen halofuginone lactate lipid kinase inhibitors nitazoxanide anti-parasitics paromomycin cryptosporidiosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	This study evaluated the effectiveness of anti-cryptosporidial agents nitazoxanide, halofuginone, the pyrazolopyridine analog KDU731, and paromomycin (PMC) in combating the significant zoonotic pathogen . The study utilized HCT-8 host cells to culture and fluorescent microscopy/quantitative PCR (qPCR) for detecting parasitic growth. The efficacy of the compounds was assessed by calculating their inhibitory concentrations (IC) against the total growth of at 48 h post-infection. The study further investigated the impact of these compounds on early parasitophorous vacuole (PV) formation, merozoite egress, host cell viability, and cell growth cycle. KDU731 displayed the most promising profile, with low nanomolar (102 nM ± 2.28) activity and negligible host cell toxicity. This study offers new insights into the relative efficacy and safety of various anti-cryptosporidial compounds, highlighting their stage-specific effects on and the consequential impacts on host cells. Identifying safe and effective anti-cryptosporidial agents contributes significantly to the One Health approach, which emphasizes the importance of integrated strategies in controlling zoonotic diseases.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1664-302X 1664-302X
DOI:	10.3389/fmicb.2024.1463457