Dissection of maize kernel composition and starch production by candidate gene association

Dissection of maize kernel composition and starch production by candidate gene association

Cereal starch production forms the basis of subsistence for much of the world's human and domesticated animal populations. Starch concentration and composition in the maize (Zea mays ssp mays) kernel are complex traits controlled by many genes. In this study, an association approach was used to...

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Bibliographic Details
Published in:The Plant cell Vol. 16; no. 10; pp. 2719 - 2733
Main Authors: Wilson, L.M, Whitt, S.R, Ibanez, A.M, Rocheford, T.R, Goodman, M.M, Buckler, E.S. IV
Format: Journal Article
Language:English
Published: United States American Society of Plant Biologists 01-10-2004
Subjects:
Alleles
amylose
Animal populations
Base Sequence
Biosynthesis
carbohydrate composition
carbohydrate content
carbohydrate metabolism
Corn
DNA Primers
Domestic animals
Exons
Genes, Plant
Genetic Linkage
Haplotypes
Introns
major genes
Molecular Sequence Data
nucleotide sequences
pasting properties
Phenotypic traits
Plants
Population structure
Quantitative Trait Loci
Starch
Starch - biosynthesis
Starches
Viscosity
Zea mays
Zea mays - anatomy & histology
Zea mays - genetics
Zea mays - metabolism
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Summary:Cereal starch production forms the basis of subsistence for much of the world's human and domesticated animal populations. Starch concentration and composition in the maize (Zea mays ssp mays) kernel are complex traits controlled by many genes. In this study, an association approach was used to evaluate six maize candidate genes involved in kernel starch biosynthesis: amylose extender1 (ae1), brittle endosperm2 (bt2), shrunken1 (sh1), sh2, sugary1, and waxy1. Major kernel composition traits, such as protein, oil, and starch concentration, were assessed as well as important starch composition quality traits, including pasting properties and amylose levels. Overall, bt2, sh1, and sh2 showed significant associations for kernel composition traits, whereas ae1 and sh2 showed significant associations for starch pasting properties. ae1 and sh1 both associated with amylose levels. Additionally, haplotype analysis of sh2 suggested this gene is involved in starch viscosity properties and amylose content. Despite starch concentration being only moderately heritable for this particular panel of diverse maize inbreds, high resolution was achieved when evaluating these starch candidate genes, and diverse alleles for breeding and further molecular analysis were identified.
Bibliography:http://hdl.handle.net/10113/26857
http://dx.doi.org/10.1105/tpc.104.025700
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To whom correspondence should be addressed. E-mail esb33@cornell.edu; fax 607-255-6249.
Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.104.025700.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Edward S. Buckler (esb33@cornell.edu).
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.104.025700