Search Results - "Whitman, C. P."

The chemical versatility of the β–α–β fold: Catalytic promiscuity and divergent evolution in the tautomerase superfamily by Poelarends, G. J., Veetil, V. Puthan, Whitman, C. P.

“… Tautomerase superfamily members have an amino-terminal proline and a β–α–β fold, and include 4-oxalocrotonate tautomerase (4-OT),…”
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Probing the Oligomeric Structure of an Enzyme by Electrospray Ionization Time-Of-Flight Mass Spectrometry by Fitzgerald, Michael C., Chernushevich, Igor, Standing, Kenneth G., Whitman, Christian P., Stephen B. H. Kent

“…Electrospray ionization time-of-flight (ESITOF) mass spectrometry was used to study the quaternary structure of 4-oxalocrotonate tautomerase (EC 5.3.2; 4OT),…”
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4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer by CHEN, L. H, KENYON, G. L, CURTIN, F, HARAYAMA, S, BEMBENEK, M. E, GHOLAMHOSSEIN HAJIPOUR, WHITMAN, C. P

“…The xylH gene encoding 4-oxalocrotonate tautomerase (4-OT) has been located on a subclone of the Pseudomonas putida mt-2 TOL plasmid pWW0 and inserted into an…”
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Mechanism of the Phenylpyruvate Tautomerase Activity of Macrophage Migration Inhibitory Factor:  Properties of the P1G, P1A, Y95F, and N97A Mutants by Stamps, Stacy L, Taylor, Alexander B, Wang, Susan C, Hackert, Marvin L, Whitman, Christian P

“…Phenylpyruvate tautomerase (PPT) has been studied periodically since its activity was first described over forty years ago. In the last two years, the…”
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The Structural Basis for the Perturbed pKa of the Catalytic Base in 4-Oxalocrotonate Tautomerase:  Kinetic and Structural Effects of Mutations of Phe-50 by CZERWINSKI, Robert M., HARRIS, Thomas K., MASSIAH, Michael A., MILDVAN, Albert S., WHITMAN, Christian P.

“…The amino-terminal proline of 4-oxalocrotonate tautomerase (4-OT) functions as the general base catalyst in the enzyme-catalyzed isomerization of…”
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4‐Oxalocrotonate tautomerase, a 41‐kDa homohexamer: Backbone and side‐chain resonance assignments, solution secondary structure, and location of active site residues by heteronuclear NMR spectroscopy by Stivers, James T., Abeygunawardana, Chitrananda, Whitman, Christian P., Mildvan, Albert S.

“…4‐Oxalocrotonate tautomerase (4‐OT), a homohexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated α‐keto acids using Pro‐1…”
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Differential toxicities of cyclophosphamide and its glutathione metabolites to A549 cells by Perry, C.S., Liu, X., Lund, L.G., Whitman, C.P., Kehrer, J.P.

“…Cyclophosphamide (CP), a widely used antineoplastic agent, is metabolized to species responsible for both the therapeutic and toxic effects of this drug…”
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The X-ray Structure of trans-3-Chloroacrylic Acid Dehalogenase Reveals a Novel Hydration Mechanism in the Tautomerase Superfamily by de Jong, René M., Brugman, Wim, Poelarends, Gerrit J., Whitman, Christian P., Dijkstra, Bauke W.

“…Isomer-specific 3-chloroacrylic acid dehalogenases function in the bacterial degradation of 1,3-dichloropropene, a compound used in agriculture to kill…”
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Mechanistic characterization of a bacterial malonate semialdehyde decarboxylase: identification of a new activity on the tautomerase superfamily by Poelarends, Gerrit J, Johnson, Jr, William H, Murzin, Alexey G, Whitman, Christian P

“…Malonate semialdehyde decarboxylase (MSAD) has been identified as the protein encoded by the orf130 gene from Pseudomonas pavonaceae 170 on the basis of the…”
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Oral administration of naturally occurring coumarins leads to altered phase I and II enzyme activities and reduced DNA adduct formation by polycyclic aromatic hydrocarbons in various tissues of SENCAR mice by Kleiner, Heather E., Vulimiri, Suryanarayana V., Miller, Laura, Johnson, William H., Whitman, Christian P., DiGiovanni, John

“…Several naturally occurring coumarins, to which humans are routinely exposed in the diet, were previously found to inhibit P450-mediated metabolism of…”
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Enzymatically Inactive Macrophage Migration Inhibitory Factor Inhibits Monocyte Chemotaxis and Random Migration by Hermanowski-Vosatka, Anne, Mundt, Steven S, Ayala, Julia M, Goyal, Shefali, Hanlon, William A, Czerwinski, Robert M, Wright, Samuel D, Whitman, Christian P

“…Macrophage migration inhibitory factor (MIF) is a cytokine that was first described as an inhibitor of the random migration of monocytes and macrophages and…”
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Crystal Structures of the Wild-Type, P1A Mutant, and Inactivated Malonate Semialdehyde Decarboxylase:  A Structural Basis for the Decarboxylase and Hydratase Activities by Almrud, Jeffrey J, Poelarends, Gerrit J, Johnson, William H, Serrano, Hector, Hackert, Marvin L, Whitman, Christian P

“…Malonate semialdehyde decarboxylase (MSAD) from Pseudomonas pavonaceae 170 is a tautomerase superfamily member that converts malonate semialdehyde to…”
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Preliminary analysis of crystals of 4-oxalocrotonate tautomerase, an enzyme composed of unusually small monomers by Davenport, R C, Whitman, C P

“…Crystals of 4-oxalocrotonate tautomerase have been obtained by the vapor-diffusion method using polyethylene glycol as precipitant. The crystals belong to the…”
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Characterization of the Role of the Amino-Terminal Proline in the Enzymatic Activity Catalyzed by Macrophage Migration Inhibitory Factor by Stamps, Stacy L, Fitzgerald, Michael C, Whitman, Christian P

“…The cytokine macrophage migration inhibitory factor (MIF) mediates several immune and inflammatory processes through unknown or poorly understood mechanisms…”
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Kinetic, Stereochemical, and Structural Effects of Mutations of the Active Site Arginine Residues in 4-Oxalocrotonate Tautomerase by Harris, Thomas K, Czerwinski, Robert M, Johnson, William H, Legler, Patricia M, Abeygunawardana, Chitrananda, Massiah, Michael A, Stivers, James T, Whitman, Christian P, Mildvan, Albert S

“…Three arginine residues (Arg-11, Arg-39, Arg-61) are found at the active site of 4-oxalocrotonate tautomerase in the X-ray structure of the affinity-labeled…”
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The Crystal Structure of YdcE, a 4-Oxalocrotonate Tautomerase Homologue from Escherichia coli, Confirms the Structural Basis for Oligomer Diversity by Almrud, Jeffrey J, Kern, Andrew D, Wang, Susan C, Czerwinski, Robert M, Johnson, William H, Murzin, Alexey G, Hackert, Marvin L, Whitman, Christian P

“…The tautomerase superfamily consists of three major families represented by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase…”
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Catalytic Role of the Amino-Terminal Proline in 4-Oxalocrotonate Tautomerase:  Affinity Labeling and Heteronuclear NMR Studies by Stivers, James T, Abeygunawardana, Chitrananda, Mildvan, Albert S, Hajipour, Gholamhossein, Whitman, Christian P, Chen, Lorenzo H

“…4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated α-keto acids,…”
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Kinetic and Structural Effects of Mutations of the Catalytic Amino-Terminal Proline in 4-Oxalocrotonate Tautomerase by Czerwinski, Robert M, Johnson, William H, Whitman, Christian P, Harris, Thomas K, Abeygunawardana, Chitrananda, Mildvan, Albert S

“…The catalytic general base, Pro-1, of the enzyme 4-oxalocrotonate tautomerase has been mutated to Gly, Ala, Val, and Leu, residues with aliphatic side chains…”
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Secretory leukocyte protease inhibitor: inhibition of human immunodeficiency virus-1 infection of monocytic THP-1 cells by a newly cloned protein by Shine, Nancy R, Wang, Susan C, Konopka, Krystyna, Burks, Elizabeth A, Düzgüneş, Nejat, Whitman, Christian P

“…The ability of the salivary protein, secretory leukocyte protease inhibitor (SLPI), to inhibit human immunodeficiency virus-1 (HIV-1) infection in vitro has…”
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Inactivation of 4-Oxalocrotonate Tautomerase by 2-Oxo-3-pentynoate by Johnson, William H, Czerwinski, Robert M, Fitzgerald, Michael C, Whitman, Christian P

“…The compound, 2-oxo-3-pentynoate, has been synthesized and tested as an inhibitor of the enzyme 4-oxalocrotonate tautomerase. The enzyme is rapidly and…”
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