Exploiting Knowledge of R/Avr Genes to Rapidly Clone a New LZ-NBS-LRR Family of Late Blight Resistance Genes from Potato Linkage Group IV

In addition to the resistance to Phytophthora infestans (Rpi) genes Rpi-blb1 and Rpi-blb2, Solanum bulbocastanum appears to harbor Rpi-blb3 located at a major late blight resistance locus on LG IV, which also harbors Rpi-abpt, R2, R2-like, and Rpi-mcd1 in other Solanum spp. Here, we report the cloni...

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Published in:	Molecular plant-microbe interactions Vol. 22; no. 6; pp. 630 - 641
Main Authors:	Lokossou, Anoma A, Park, Tae-ho, Arkel, Gert van, Arens, Marjon, Ruyter-Spira, Carolien, Morales, Juan, Whisson, Steve C, Birch, Paul R.J, Visser, Richard G.F, Jacobsen, Evert, Vossen, Edwin A.G. van der
Format:	Journal Article
Language:	English
Published:	St. Paul, MN APS Press 01-06-2009 The American Phytopathological Society
Subjects:	Alleles Amino Acid Sequence amino acid sequences arabidopsis-thaliana Biological and medical sciences broad-spectrum resistance Cloning, Molecular disease resistance downy mildew Fundamental and applied biological sciences. Psychology fungal diseases of plants genes Genetic Complementation Test genetic polymorphism genetic resistance Immunity, Innate - genetics linkage groups Molecular Sequence Data Multigene Family Nicotiana - genetics nucleotide sequences oomycete effectors Phylogeny Phytopathology. Animal pests. Plant and forest protection Phytophthora infestans phytophthora-infestans mont Plant Diseases - genetics Plant Diseases - microbiology plant immune-system plant pathogenic fungi plant proteins Plant Proteins - chemistry Plant Proteins - genetics Plants, Genetically Modified - microbiology potatoes protein subunits quantitative resistance r-gene Sequence Alignment Solanum bulbocastanum Solanum tuberosum Solanum tuberosum - genetics wild relatives Resistance Gene Microbiology Solanum tuberosum Dicotyledones Angiospermae Spermatophyta Tuber plant Solanaceae
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Summary:	In addition to the resistance to Phytophthora infestans (Rpi) genes Rpi-blb1 and Rpi-blb2, Solanum bulbocastanum appears to harbor Rpi-blb3 located at a major late blight resistance locus on LG IV, which also harbors Rpi-abpt, R2, R2-like, and Rpi-mcd1 in other Solanum spp. Here, we report the cloning and functional analyses of four Rpi genes, using a map-based cloning approach, allele-mining strategy, Gateway technology, and transient complementation assays in Nicotiana benthamiana. Rpi-blb3, Rpi-abpt, R2, and R2-like contain all signature sequences characteristic of leucine zipper nucleotide binding site leucine-rich repeat (LZ-NBS-LRR) proteins, and share amino-acid sequences 34.9% similar to RPP13 from Arabidopsis thaliana. The LRR domains of all four Rpi proteins are highly homologous whereas LZ and NBS domains are more polymorphic, those of R2 being the most divergent. Clear blocks of sequence affiliation between the four functional resistance proteins and those encoded by additional Rpi-blb3 gene homologs suggest exchange of LZ, NBS, and LRR domains, underlining the modular nature of these proteins. All four Rpi genes recognize the recently identified RXLR effector PiAVR2.
Bibliography:	http://dx.doi.org/10.1094/MPMI-22-6-0630 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0894-0282 1943-7706
DOI:	10.1094/mpmi-22-6-0630