Preparation and purification of cationic solid lipid nanospheres—effects on particle size, physical stability and cell toxicity

Cationic solid lipid nanospheres (SLN) were prepared by the microemulsion technique with polysorbate 80 (Tween 80) and butanol as surfactants. The SLN (diameter 100–500 nm, zetapotential around +15 mV) consisted mainly of stearylamine (SA) and different triglycerides. Three different purification me...

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Bibliographic Details
Published in:	International journal of pharmaceutics Vol. 254; no. 1; pp. 83 - 87
Main Authors:	Heydenreich, A.V., Westmeier, R., Pedersen, N., Poulsen, H.S., Kristensen, H.G.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Amsterdam Elsevier B.V 18-03-2003 Elsevier
Subjects:	Biological and medical sciences Carcinoma, Small Cell - pathology Cationic Cell Line Cell toxicity Chemistry, Pharmaceutical Dialysis - methods Drug Stability Drug-Related Side Effects and Adverse Reactions General pharmacology Humans Lipids - chemistry Liposomes Maximum Tolerated Dose Medical sciences Microspheres Particle Size Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polysorbate 80 Polysorbates - metabolism Solid lipid nanospheres Stearylamine Surface-Active Agents Surfactants Time Factors Ultracentrifugation - instrumentation Ultracentrifugation - methods Ultrafiltration - methods Cell toxicity Solid lipid nanospheres Stearylamine Polysorbate 80 Surfactants Cationic Particle size Toxicity Lipids Drug carrier Ultracentrifugation Surfactant Ultrafiltration Nanosphere Microemulsion Sorbate polymer Delivery system Purification Stability Pharmaceutical technology Triglyceride In vitro Dosage form Butanol Dialysis
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Summary:	Cationic solid lipid nanospheres (SLN) were prepared by the microemulsion technique with polysorbate 80 (Tween 80) and butanol as surfactants. The SLN (diameter 100–500 nm, zetapotential around +15 mV) consisted mainly of stearylamine (SA) and different triglycerides. Three different purification methods, ultrafiltration, ultracentrifugation and dialysis, were investigated and compared with the cellular toxicity and physical stability of the dispersions. The cell toxicity was dependent on both the SLN composition and the purification method. Dialysis was found to easily and efficiently remove excessive surfactant determined by dynamic light scattering (DLS), leading to reduced cell toxicity and increased physical stability of the SLN on storage. The cationic SLN might constitute a promising DNA delivery system.
ISSN:	0378-5173 1873-3476
DOI:	10.1016/S0378-5173(02)00688-9