Search Results - "Westh, Peter"
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Sabatier Principle for Interfacial (Heterogeneous) Enzyme Catalysis
Published in ACS catalysis (07-12-2018)“…The Sabatier principle states that optimal catalysis occurs when interactions between catalyst and substrate are of intermediary strength. Although qualitative…”
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Displacement of Drugs From Cyclodextrin Complexes by Bile Salts: A Suggestion of an Intestinal Drug-Solubilizing Capacity From an In Vitro Model
Published in Journal of pharmaceutical sciences (01-09-2016)“…The dosing of drugs in an aqueous cyclodextrin formulation requires sufficient amount of cyclodextrins to fully solubilize the drug, as described by Stella's…”
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Transient Kinetics and Rate-Limiting Steps for the Processive Cellobiohydrolase Cel7A: Effects of Substrate Structure and Carbohydrate Binding Domain
Published in Biochemistry (Easton) (10-12-2013)“…Cellobiohydrolases are exoacting, processive enzymes that effectively hydrolyze crystalline cellulose. They have attracted considerable interest because of…”
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Sabatier Principle for Rationalizing Enzymatic Hydrolysis of a Synthetic Polyester
Published in JACS Au (23-05-2022)“…Interfacial enzyme reactions are common in Nature and in industrial settings, including the enzymatic deconstruction of poly(ethylene terephthalate) (PET)…”
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Interfacial Catalysis during Amylolytic Degradation of Starch Granules: Current Understanding and Kinetic Approaches
Published in Molecules (Basel, Switzerland) (28-04-2023)“…Enzymatic hydrolysis of starch granules forms the fundamental basis of how nature degrades starch in plant cells, how starch is utilized as an energy resource…”
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Reconciliation of opposing views on membrane-sugar interactions
Published in Proceedings of the National Academy of Sciences - PNAS (01-02-2011)“…It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of…”
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Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A
Published in The Journal of biological chemistry (25-05-2012)“…The transient kinetic behavior of enzyme reactions prior to the establishment of steady state is a major source of mechanistic information, yet this approach…”
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A versatile microbial platform as a tunable whole-cell chemical sensor
Published in Nature communications (27-09-2024)“…Biosensors are used to detect and quantify chemicals produced in industrial microbiology with high specificity, sensitivity, and portability. Most biosensors,…”
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How Much Weaker Are the Effects of Cations than Those of Anions? The Effects of K+ and Cs+ on the Molecular Organization of Liquid H2O
Published in The journal of physical chemistry. B (24-07-2014)“…We characterized the effects of K+ and Cs+ ions on the molecular organization of H2O by the 1-propanol probing methodology, previously developed by us (Phys…”
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Rate‐limiting step and substrate accessibility of cellobiohydrolase Cel6A from Trichoderma reesei
Published in The FEBS journal (01-12-2018)“…The cellobiohydrolase (CBH) Cel6A is an important component of enzyme cocktails for industrial degradation of lignocellulosic biomass. However, the kinetics of…”
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The structural basis of fungal glucuronoyl esterase activity on natural substrates
Published in Nature communications (24-02-2020)“…Structural and functional studies were conducted of the glucuronoyl esterase (GE) from Cerrena unicolor ( Cu GE), an enzyme catalyzing cleavage of…”
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steady‐state theory for processive cellulases
Published in The FEBS journal (01-08-2013)“…Processive enzymes perform sequential steps of catalysis without dissociating from their polymeric substrate. This mechanism is considered essential for…”
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Free Energy Diagram for the Heterogeneous Enzymatic Hydrolysis of Glycosidic Bonds in Cellulose
Published in The Journal of biological chemistry (04-09-2015)“…Kinetic and thermodynamic data have been analyzed according to transition state theory and a simplified reaction scheme for the enzymatic hydrolysis of…”
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Surface display as a functional screening platform for detecting enzymes active on PET
Published in Microbial cell factories (01-05-2021)“…Poly(ethylene terephthalate) (PET) is the world's most abundant polyester plastic, and its ongoing accumulation in nature is causing a global environmental…”
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Determination of the aggregation number for micelles by isothermal titration calorimetry
Published in Thermochimica acta (20-07-2014)“…•A new quantification strategy is developed to determine the aggregation number.•The method circumvents problems with parameter correlation and heat of…”
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Effects of PEG size on structure, function and stability of PEGylated BSA
Published in European journal of pharmaceutics and biopharmaceutics (01-10-2011)“…The binding of surfactant, SDS, to BSA appears to be altered upon PEGylation, as two distinct classes of binding sites (high and low affinity) for BSA and one…”
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kinetic model for the burst phase of processive cellulases
Published in The FEBS journal (01-05-2011)“…Cellobiohydrolases (exocellulases) hydrolyze cellulose processively, i.e. by sequential cleaving of soluble sugars from one end of a cellulose strand. Their…”
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Purification and biochemical characterization of SM14est, a PET-hydrolyzing enzyme from the marine sponge-derived Streptomyces sp. SM14
Published in Frontiers in microbiology (12-05-2023)“…The successful enzymatic degradation of polyester substrates has fueled worldwide investigation into the treatment of plastic waste using bio-based processes…”
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Physical constraints and functional plasticity of cellulases
Published in Nature communications (22-06-2021)“…Enzyme reactions, both in Nature and technical applications, commonly occur at the interface of immiscible phases. Nevertheless, stringent descriptions of…”
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Is a Methyl Group Always Hydrophobic? Hydrophilicity of Trimethylamine-N-oxide, Tetramethyl Urea and Tetramethylammonium Ion
Published in The journal of physical chemistry. B (31-03-2011)“…We have developed what we call the 1-propanol(1P) probing methodology. By using it, we determined the relative hydrophobicity/hydrophilicity indices of…”
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