Purification, Expression and Binding Properties of BC1 Ribonucleoproteins
Brain Cytoplasmic 1 (BC1) is a member of the short interspersed repetitive elements (SINEs) in rodent genomes. This class of elements is able to retropose itself and reinsert in the host genome. Like most SINEs, BC1 is transcribed by RNA polymerase III, less than 300 base pairs long, has evolved fro...
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| Format: | Dissertation |
| Language: | English |
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ProQuest Dissertations & Theses
01-01-2000
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| Online Access: | Get full text |
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| Summary: | Brain Cytoplasmic 1 (BC1) is a member of the short interspersed repetitive elements (SINEs) in rodent genomes. This class of elements is able to retropose itself and reinsert in the host genome. Like most SINEs, BC1 is transcribed by RNA polymerase III, less than 300 base pairs long, has evolved from a tRNA precursor, and codes for a small, untranslated RNA that lacks coding capacity. Unlike other SINEs, the RNA coded for in BC1 has tissue-specific expression patterns. BC1 RNA is expressed only in brain and testis and in muscles just after synaptogenesis. Within the brain, BC1 is expressed only in neurons. Within neurons, BC1 RNA is found only in the somatodendritic compartment. Within dendrites, BC1 RNA accumulates in synaptodendrosomes. There is evidence that BC1 can also be regulated by stimulation changes within these neurons. The BC1 RNA does not exist in these areas as a bare RNA; it is bound by proteins to form a ribonucleoprotein particle (RNP). The purpose of this dissertation is to elucidate properties of these unknown proteins. The tissue specificity of the upstream sequences of the BC1 gene is examined in Chapter 1 with the generation of a transgenic mouse. The sequences within the RNA that bind to proteins are the subject of Chapter 2. Chapter 3 outlines the purification scheme used to determine several protein candidates and that will be used to purify transgenic brain in the future. The final chapter addresses protein candidates published and unpublished that may bind BC1 RNA. |
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| ISBN: | 0599966483 9780599966482 |