Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diver...

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Bibliographic Details
Published in:Crop breeding and applied biotechnology Vol. 16; no. 3; pp. 219 - 225
Main Authors: Guerra, Divanilde, Souza, Paulo Vitor Dutra de, Schwarz, Sérgio Francisco, Schifino-Wittmann, Maria Teresa, Werlang, Claudio André, Veit, Pedro Augusto
Format: Journal Article
Language:English
Published: Vicosa Crop Breeding and Applied Biotechnology 01-09-2016
Brazilian Society of Plant Breeding
Subjects:
AGRONOMY
Anthers
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Breeding
cytogenetic analysis
Deoxyribonucleic acid
Diploids
DNA
Flowers
Fruit trees
Gene polymorphism
Genetic diversity
Germination
Hybridization
hybridizations
In vitro methods and tests
Meiosis
molecular analysis
Plant diversity
Plantations
Pollen
Polymerase chain reaction
Polymorphism
Random amplified polymorphic DNA
Similarity
Stability analysis
Trees
Viability
Rio Grande do Sul Brazil
hybridizations
cytogenetic analysis
Breeding
molecular analysis
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Summary:This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was 82.12%; average pollen viability was 92.44% and in vitro germination was 40.26%; the average number of anthers was 161.85 anthers/flowers. Therefore, the accessions evaluated showed high genetic similarity and cytological stability and can be used in commercial plantations or hybridizations.
ISSN:1984-7033
1518-7853
1984-7033
DOI:10.1590/1984-70332016v16n3a33