Search Results - "Weng, Steve"

Large-scale production of stem cells utilizing microcarriers: A biomaterials engineering perspective from academic research to commercialized products by Tavassoli, Hossein, Alhosseini, Sanaz Naghavi, Tay, Andy, Chan, Peggy P.Y., Weng Oh, Steve Kah, Warkiani, Majid Ebrahimi

“…Human stem cells, including pluripotent, embryonic and mesenchymal, stem cells play pivotal roles in cell-based therapies. Over the past decades, various…”
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Human mesenchymal stem cell therapy for cartilage repair: Review on isolation, expansion, and constructs by Lam, Alan T.L., Reuveny, Shaul, Oh, Steve Kah-Weng

“…●Articular cartilage has a poor intrinsic capacity for self-repair.●Mesenchymal stem cell (MSC)-based therapy for cartilage regeneration.●Bioprocessing…”
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Industrially Compatible Transfusable iPSC-Derived RBCs: Progress, Challenges and Prospective Solutions by Lim, Zhong Ri, Vassilev, Svetlan, Leong, Yew Wai, Hang, Jing Wen, Rénia, Laurent, Malleret, Benoit, Oh, Steve Kah-Weng

“…Amidst the global shortfalls in blood supply, storage limitations of donor blood and the availability of potential blood substitutes for transfusion…”
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KLF4 and PBX1 Directly Regulate NANOG Expression in Human Embryonic Stem Cells by Chan, Ken Kwok‐Keung, Zhang, Jingyao, Chia, Na‐Yu, Chan, Yun‐Shen, Sim, Hui Shan, Tan, Ker Sin, Oh, Steve Kah‐Weng, Ng, Huck‐Hui, Choo, Andre Boon‐Hwa

“…Insight into the regulation of core transcription factors is important for a better understanding of the molecular mechanisms that control self‐renewal and…”
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Selection of human induced pluripotent stem cells lines optimization of cardiomyocytes differentiation in an integrated suspension microcarrier bioreactor by Laco, Filip, Lam, Alan Tin-Lun, Woo, Tsung-Liang, Tong, Gerine, Ho, Valerie, Soong, Poh-Loong, Grishina, Elina, Lin, Kun-Han, Reuveny, Shaul, Oh, Steve Kah-Weng

“…The production of large quantities of cardiomyocyte is essential for the needs of cellular therapies. This study describes the selection of a human-induced…”
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Integrated processes for expansion and differentiation of human pluripotent stem cells in suspended microcarriers cultures by Lam, Alan Tin-Lun, Chen, Allen Kuan-Liang, Ting, Sherwin Qi-Peng, Reuveny, Shaul, Oh, Steve Kah-Weng

“…Current methods for human pluripotent stem cells (hPSC) expansion and differentiation can be limited in scalability and costly (due to their labor intensive…”
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Improved erythroid differentiation of multiple human pluripotent stem cell lines in microcarrier culture by modulation of Wnt/β-Catenin signaling by Sivalingam, Jaichandran, Chen, Hong Yu, Yang, Bin-Xia, Lim, Zhong Ri, Lam, Alan Tin Lun, Woo, Tsung Liang, Chen, Allen Kuan-Liang, Reuveny, Shaul, Loh, Yuin-Han, Oh, Steve Kah-Weng

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Defined Serum‐Free Medium for Bioreactor Culture of an Immortalized Human Erythroblast Cell Line by Lee, Esmond, Lim, Zhong Ri, Chen, Hong‐Yu, Yang, Bin Xia, Lam, Alan Tin‐Lun, Chen, Allen Kuan‐Liang, Sivalingam, Jaichandran, Reuveny, Shaul, Loh, Yuin‐Han, Oh, Steve Kah‐Weng

“…Anticipated shortages in donated blood supply have prompted investigation of alternative approaches for in vitro production of red blood cells (RBCs), such as…”
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Translating human embryonic stem cells from 2-dimensional to 3-dimensional cultures in a defined medium on laminin- and vitronectin-coated surfaces by Heng, Boon Chin, Li, Jian, Chen, Allen Kuan-Liang, Reuveny, Shaul, Cool, Simon M, Birch, William R, Oh, Steve Kah-Weng

“…While defining the environment for human embryonic stem cell (hESC) culture on 2-dimensional (2D) surfaces has made rapid progress, the industrial-scale…”
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Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture by Lam, Alan Tin-Lun, Chen, Allen Kuan-Liang, Li, Jian, Birch, William R, Reuveny, Shaul, Oh, Steve Kah-Weng

“…Myocardial infarction is accompanied by a significant loss of cardiomyocytes (CMs). Functional CMs, differentiated from human embryonic stem cells (hESCs),…”
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Characterizing Escherichia coli DH5α growth and metabolism in a complex medium using genome-scale flux analysis by Selvarasu, Suresh, Ow, Dave Siak-Wei, Lee, Sang Yup, Lee, May May, Oh, Steve Kah-Weng, Karimi, Iftekhar A., Lee, Dong-Yup

“…Genome‐scale flux analysis of Escherichia coli DH5α growth in a complex medium was performed to investigate the relationship between the uptake of various…”
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Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction by Chen, Allen Kuan-Liang, Reuveny, Shaul, Oh, Steve Kah Weng

“…Mesenchymal stem cells (MSCs) have recently made significant progress with multiple clinical trials targeting modulation of immune responses, regeneration of…”
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Identification of cellular objective for elucidating the physiological state of plasmid-bearing Escherichia coli using genome-scale in silico analysis by Ow, Dave Siak-Wei, Lee, Dong-Yup, Yap, Miranda Gek-Sim, Oh, Steve Kah-Weng

“…The presence of multiple copies of plasmids in Escherichia coli could induce a complex cascade of physiological changes known as the metabolic burden response…”
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Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures by Lam, Alan Tin-Lun, Li, Jian, Chen, Allen Kuan-Liang, Birch, William R, Reuveny, Shaul, Oh, Steve Kah-Weng

“…Human pluripotent stem cells (hPSC) are self-renewing cells having the potential of differentiation into the three lineages of somatic cells and thus can be…”
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Recent advances in serum-free microcarrier expansion of mesenchymal stromal cells: Parameters to be optimized by Tan, Kah Yong, Reuveny, Shaul, Oh, Steve Kah Weng

“…Mesenchymal stromal cells (MSCs) are being investigated for a variety of therapeutic indications. However, current 2D planar technology cannot meet the…”
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Inactivating FruR global regulator in plasmid-bearing Escherichia coli alters metabolic gene expression and improves growth rate by Ow, Dave Siak-Wei, Lee, Raymond Ming-Yung, Nissom, Peter Morin, Philp, Robin, Oh, Steve Kah-Weng, Yap, Miranda Gek-Sim

“…The introduction of plasmids into Escherichia coli is known to impose a metabolic burden, which diminishes the growth rate. This effect could arise from…”
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Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges by Lee, Esmond, Sivalingam, Jaichandran, Lim, Zhong Ri, Chia, Gloryn, Shi, Low Gin, Roberts, Mackenna, Loh, Yuin-Han, Reuveny, Shaul, Oh, Steve Kah-Weng

“…In vitro generation of red blood cells (RBCs) has the potential to circumvent the shortfalls in global demand for blood for transfusion applications. The…”
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Selection of O‐negative induced pluripotent stem cell clones for high‐density red blood cell production in a scalable perfusion bioreactor system by Yu, SuE, Vassilev, Svetlan, Lim, Zhong Ri, Sivalingam, Jaichandran, Lam, Alan Tin Lun, Ho, Valerie, Renia, Laurent, Malleret, Benoit, Reuveny, Shaul, Oh, Steve Kah Weng

“…Objectives Large‐scale generation of universal red blood cells (RBCs) from O‐negative (O‐ve) human induced pluripotent stem cells (hiPSCs) holds the potential…”
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An allied reprogramming, selection, expansion and differentiation platform for creating hiPSC on microcarriers by Lam, Alan Tin Lun, Ho, Valerie, Vassilev, Svetlan, Reuveny, Shaul, Oh, Steve Kah Weng

“…Objectives Induced pluripotent stem cells (iPSCs) generated by monolayer cultures is plagued by low efficiencies, high levels of manipulation and operator…”
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Increasing efficiency of human mesenchymal stromal cell culture by optimization of microcarrier concentration and design of medium feed by Chen, Allen Kuan-Liang, Chew, Yi Kong, Tan, Hong Yu, Reuveny, Shaul, Weng Oh, Steve Kah

“…Abstract Background aims Large amounts of human mesenchymal stromal cells (MSCs) are needed for clinical cellular therapy. In a previous publication, we…”
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