Search Results - "Wells, James A."

Development of Antibody-Based PROTACs for the Degradation of the Cell-Surface Immune Checkpoint Protein PD-L1 by Cotton, Adam D, Nguyen, Duy P, Gramespacher, Josef A, Seiple, Ian B, Wells, James A

“…Targeted protein degradation has emerged as a new paradigm to manipulate cellular proteostasis. Proteolysis-targeting chimeras (PROTACs) are bifunctional small…”
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K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions by Ostrem, Jonathan M., Peters, Ulf, Sos, Martin L., Wells, James A., Shokat, Kevan M.

“…Small molecules are developed that irreversibly bind to the common G12C mutant of K-Ras but not the wild-type protein; crystallographic studies reveal the…”
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Caspase Substrates and Cellular Remodeling by Crawford, Emily D, Wells, James A

“…The caspases are unique proteases that mediate the major morphological changes of apoptosis and various other cellular remodeling processes. As we catalog and…”
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Quantitative Proteomics Reveal a Feedforward Mechanism for Mitochondrial PARKIN Translocation and Ubiquitin Chain Synthesis by Ordureau, Alban, Sarraf, Shireen A., Duda, David M., Heo, Jin-Mi, Jedrychowski, Mark P., Sviderskiy, Vladislav O., Olszewski, Jennifer L., Koerber, James T., Xie, Tiao, Beausoleil, Sean A., Wells, James A., Gygi, Steven P., Schulman, Brenda A., Harper, J. Wade

“…Phosphorylation is often used to promote protein ubiquitylation, yet we rarely understand quantitatively how ligase activation and ubiquitin (UB) chain…”
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Sexually Dimorphic Neurons in the Ventromedial Hypothalamus Govern Mating in Both Sexes and Aggression in Males by Yang, Cindy F., Chiang, Michael C., Gray, Daniel C., Prabhakaran, Mahalakshmi, Alvarado, Maricruz, Juntti, Scott A., Unger, Elizabeth K., Wells, James A., Shah, Nirao M.

“…Sexual dimorphisms in the brain underlie behavioral sex differences, but the function of individual sexually dimorphic neuronal populations is poorly…”
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Redox-based reagents for chemoselective methionine bioconjugation by Lin, Shixian, Yang, Xiaoyu, Jia, Shang, Weeks, Amy M., Hornsby, Michael, Lee, Peter S., Nichiporuk, Rita V., Iavarone, Anthony T., Wells, James A., Toste, F. Dean, Chang, Christopher J.

“…Cysteine can be specifically functionalized by a myriad of acid-base conjugation strategies for applications ranging from probing protein function to…”
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Engineered ACE2 receptor traps potently neutralize SARS-CoV-2 by Glasgow, Anum, Glasgow, Jeff, Limonta, Daniel, Solomon, Paige, Lui, Irene, Zhang, Yang, Nix, Matthew A., Rettko, Nicholas J., Zha, Shoshana, Yamin, Rachel, Kao, Kevin, Rosenberg, Oren S., Ravetch, Jeffrey V., Wiita, Arun P., Leung, Kevin K., Lim, Shion A., Zhou, Xin X., Hobman, Tom C., Kortemme, Tanja, Wells, James A.

“…An essential mechanism for severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)…”
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Substrates of IAP Ubiquitin Ligases Identified with a Designed Orthogonal E3 Ligase, the NEDDylator by Zhuang, Min, Guan, Shenheng, Wang, Haopeng, Burlingame, Alma L., Wells, James A.

“…Inhibitors of Apoptosis Protein (IAPs) are guardian ubiquitin ligases that keep classic proapoptotic proteins in check. Systematic identification of additional…”
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Sampling the N-terminal proteome of human blood by Wildes, David, Wells, James A.

“…The proteomes of blood plasma and serum represent a potential gold mine of biological and diagnostic information, but challenges such as dynamic range of…”
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CryptoSite: Expanding the Druggable Proteome by Characterization and Prediction of Cryptic Binding Sites by Cimermancic, Peter, Weinkam, Patrick, Rettenmaier, T. Justin, Bichmann, Leon, Keedy, Daniel A., Woldeyes, Rahel A., Schneidman-Duhovny, Dina, Demerdash, Omar N., Mitchell, Julie C., Wells, James A., Fraser, James S., Sali, Andrej

“…Many proteins have small-molecule binding pockets that are not easily detectable in the ligand-free structures. These cryptic sites require a conformational…”
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A reactivity-based probe of the intracellular labile ferrous iron pool by Spangler, Benjamin, Morgan, Charles W, Fontaine, Shaun D, Vander Wal, Mark N, Chang, Christopher J, Wells, James A, Renslo, Adam R

“…Editorial Summary A reactivity-based probe containing an iron-sensitive 1,2,4-trixolane ring conjugated to a small molecule payload combined with a…”
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Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles by Julien, Olivier, Zhuang, Min, Wiita, Arun P., O’Donoghue, Anthony J., Knudsen, Giselle M., Craik, Charles S., Wells, James A.

“…Proteases constitute the largest enzyme family, yet their biological roles are obscured by our rudimentary understanding of their cellular substrates. There…”
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Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity by Nguyen, Duy P., Miyaoka, Yuichiro, Gilbert, Luke A., Mayerl, Steven J., Lee, Brian H., Weissman, Jonathan S., Conklin, Bruce R., Wells, James A.

“…Cas9-based RNA-guided nuclease (RGN) has emerged to be a versatile method for genome editing due to the ease of construction of RGN reagents to target specific…”
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Systematic identification of engineered methionines and oxaziridines for efficient, stable, and site-specific antibody bioconjugation by Elledge, Susanna K., Tran, Hai L., Christian, Alec H., Steri, Veronica, Hann, Byron, Toste, F. Dean, Chang, Christopher J., Wells, James A.

“…The field of chemical modification of proteins has been dominated by random modification of lysines or more site-specific labeling of cysteines, each with…”
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Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries by Zhou, Jie, Li, Shantao, Leung, Kevin K., O’Donovan, Brian, Zou, James Y., DeRisi, Joseph L., Wells, James A.

“…Proteolysis is a major posttranslational regulator of biology inside and outside of cells. Broad identification of optimal cleavage sites and natural…”
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Small-molecule inhibitors of protein-protein interactions: progressing towards the dream by Arkin, Michelle R, Wells, James A

“…Protein-protein interactions have a key role in most biological processes, and offer attractive opportunities for therapeutic intervention. Developing small…”
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Global Sequencing of Proteolytic Cleavage Sites in Apoptosis by Specific Labeling of Protein N Termini by Mahrus, Sami, Trinidad, Jonathan C., Barkan, David T., Sali, Andrej, Burlingame, Alma L., Wells, James A.

“…The nearly 600 proteases in the human genome regulate a diversity of biological processes, including programmed cell death. Comprehensive characterization of…”
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An expanded allosteric network in PTP1B by multitemperature crystallography, fragment screening, and covalent tethering by Keedy, Daniel A, Hill, Zachary B, Biel, Justin T, Kang, Emily, Rettenmaier, T Justin, Brandão-Neto, José, Pearce, Nicholas M, von Delft, Frank, Wells, James A, Fraser, James S

“…Allostery is an inherent feature of proteins, but it remains challenging to reveal the mechanisms by which allosteric signals propagate. A clearer…”
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The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28 by Muller, Yannick D, Nguyen, Duy P, Ferreira, Leonardo M R, Ho, Patrick, Raffin, Caroline, Valencia, Roxxana Valeria Beltran, Congrave-Wilson, Zion, Roth, Theodore L, Eyquem, Justin, Van Gool, Frederic, Marson, Alexander, Perez, Laurent, Wells, James A, Bluestone, Jeffrey A, Tang, Qizhi

“…Anti-CD19 chimeric antigen receptor (CD19-CAR)-engineered T cells are approved therapeutics for malignancies. The impact of the hinge domain (HD) and the…”
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Engineering a light-activated caspase-3 for precise ablation of neurons in vivo by Smart, Ashley D., Pache, Roland A., Thomsen, Nathan D., Kortemme, Tanja, Davis, Graeme W., Wells, James A.

“…The circuitry of the brain is characterized by cell heterogeneity, sprawling cellular anatomy, and astonishingly complex patterns of connectivity. Determining…”
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