Deep genome-wide measurement of meiotic gene conversion using tetrad analysis in Arabidopsis thaliana

Deep genome-wide measurement of meiotic gene conversion using tetrad analysis in Arabidopsis thaliana

Gene conversion, the non-reciprocal exchange of genetic information, is one of the potential products of meiotic recombination. It can shape genome structure by acting on repetitive DNA elements, influence allele frequencies at the population level, and is known to be implicated in human disease. Bu...

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Bibliographic Details
Published in:PLoS genetics Vol. 8; no. 10; p. e1002968
Main Authors: Sun, Yujin, Ambrose, Jonathan H, Haughey, Brena S, Webster, Tyler D, Pierrie, Sarah N, Muñoz, Daniela F, Wellman, Emily C, Cherian, Shalom, Lewis, Scott M, Berchowitz, Luke E, Copenhaver, Gregory P
Format: Journal Article
Language:English
Published: United States Public Library of Science 01-10-2012
Public Library of Science (PLoS)
Subjects:
Alleles
Arabidopsis - genetics
Arabidopsis thaliana
Biology
Chromosomes
Deoxyribonucleic acid
DNA
Gene Conversion
Genetic aspects
Genetic recombination
Genome, Plant
Genomics
Meiosis
Models, Genetic
Physiological aspects
Plant genetics
Plants, Genetically Modified
Polymorphism, Single Nucleotide
Proteins
Quantitative Trait Loci
Recombination, Genetic
Yeast
United States
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Summary:Gene conversion, the non-reciprocal exchange of genetic information, is one of the potential products of meiotic recombination. It can shape genome structure by acting on repetitive DNA elements, influence allele frequencies at the population level, and is known to be implicated in human disease. But gene conversion is hard to detect directly except in organisms, like fungi, that group their gametes following meiosis. We have developed a novel visual assay that enables us to detect gene conversion events directly in the gametes of the flowering plant Arabidopsis thaliana. Using this assay we measured gene conversion events across the genome of more than one million meioses and determined that the genome-wide average frequency is 3.5×10(-4) conversions per locus per meiosis. We also detected significant locus-to-locus variation in conversion frequency but no intra-locus variation. Significantly, we found one locus on the short arm of chromosome 4 that experienced 3-fold to 6-fold more gene conversions than the other loci tested. Finally, we demonstrated that we could modulate conversion frequency by varying experimental conditions.
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GPC is an Editor-in-Chief of PLOS Genetics.
Conceived and designed the experiments: YS LEB GPC. Performed the experiments: YS JHA BSH TDW SNP DFM ECW SC SML LEB. Analyzed the data: YS BSH GPC. Wrote the paper: YS GPC.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1002968