Search Results - "Weiserova, Marie"
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Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme
Published in PloS one (03-06-2015)“…Type I restriction-modification enzymes are multifunctional heteromeric complexes with DNA cleavage and ATP-dependent DNA translocation activities located on…”
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RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I
Published in Nucleic acids research (01-07-2008)“…The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific…”
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Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31)
Published in BMC microbiology (27-06-2008)“…Type I restriction-modification (R-M) systems are the most complex restriction enzymes discovered to date. Recent years have witnessed a renaissance of…”
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Interdomain communication in the endonuclease/motor subunit of type I restriction-modification enzyme EcoR124I
Published in Journal of molecular modeling (01-07-2014)“…Restriction-modification systems protect bacteria from foreign DNA. Type I restriction-modification enzymes are multifunctional heteromeric complexes with…”
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The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation
Published in PeerJ (San Francisco, CA) (18-01-2017)“…Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their…”
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Structural model for the multisubunit Type IC restriction–modification DNA methyltransferase M.EcoR124I in complex with DNA
Published in Nucleic acids research (01-01-2006)“…Recent publication of crystal structures for the putative DNA-binding subunits (HsdS) of the functionally uncharacterized Type I restriction–modification (R-M)…”
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The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I
Published in Journal of molecular biology (31-12-2008)“…The type I restriction–modification enzyme EcoR124I comprises three subunits with the stoichiometry HsdR2/HsdM2/HsdS1. The HsdR subunits are archetypical…”
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Antibacterial properties of Ag-doped hydroxyapatite layers prepared by PLD method
Published in Applied physics. A, Materials science & processing (01-12-2010)“…Thin hydroxyapatite (HA), silver-doped HA and silver layers were prepared using a pulsed laser deposition method. Doped layers were ablated from silver/HA…”
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Crystal structure of a novel domain of the motor subunit of the Type I restriction enzyme EcoR124 involved in complex assembly and DNA binding
Published in The Journal of biological chemistry (28-09-2018)“…Although EcoR124 is one of the better-studied Type I restriction-modification enzymes, it still presents many challenges to detailed analyses because of its…”
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Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit
Published in FEMS microbiology letters (01-05-2007)“…Abstract Phosphorylation of Type I restriction-modification (R-M) enzymes EcoKI, EcoAI, and EcoR124I – representatives of IA, IB, and IC families, respectively…”
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A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I
Published in Journal of molecular modeling (01-07-2018)“…Type I restriction-modification enzymes differ significantly from the type II enzymes commonly used as molecular biology reagents. On hemi-methylated DNAs type…”
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Cofactor induced dissociation of the multifunctional multisubunit EcoR124I investigated using electromobility shift assays, AFM and SPR
Published in RSC advances (01-01-2017)“…We have applied three techniques to the study of subunit assembly of the Type IC Restriction-Modification enzyme EcoR124I. This fully functional enzyme…”
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Coupling of endonuclease and translocase functions in EcoR124I
Published in Acta crystallographica. Section A, Foundations of crystallography (16-08-2009)“…Abstract only…”
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Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I
Published in Biological chemistry (01-04-1998)“…We have used deletion mutagenesis and PCR-based misincorporation mutagenesis to produce a collection of mutations in the central conserved region of the DNA…”
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A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage of the subunit assembly pathway
Published in Journal of molecular biology (01-12-2000)“…The HsdS subunit of a type I restriction-modification (R-M) system plays an essential role in the activity of both the modification methylase and the…”
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Cloning, production and characterisation of wild type and mutant forms of the R·EcoK endonucleases
Published in Nucleic acids research (11-02-1993)“…The hsdR, hsdM and hsdS genes coding for R·EcoK restriction endonuciease, both with and without a temperature sensitive mutation (ts-1) in the hsdS gene, were…”
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Cloning, production and characterisation of wild type and mutant forms of the R· Eco K endonucleases
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The Location of a Temperature-sensitive trans-Dominant Mutation and Its Effect on Restriction and Modification in Escherichia coli K12
Published in Journal of general microbiology (01-11-1989)“…Institute of Microbiology, Czechoslovak Academy of Sciences, Víde ská 1083, 142 20 Prague 4, Czechoslovakia Institute of Biochemistry and Physiology of…”
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Localization of the Type I Restriction–Modification Enzyme EcoKI in the Bacterial Cell
Published in Biochemical and biophysical research communications (02-04-2000)“…To localise the type I restriction–modification (R-M) enzyme EcoKI within the bacterial cell, the Hsd subunits present in subcellular fractions were analysed…”
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