Search Results - "Weis, David D"

Recommendations for the Propagation of Uncertainty in Hydrogen Exchange-Mass Spectrometric Measurements by Weis, David D

“…Hydrogen exchange-mass spectrometry (HX-MS) is used widely to characterize higher-order protein structure and to locate changes in protein structure and…”
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Reliable Identification of Significant Differences in Differential Hydrogen Exchange-Mass Spectrometry Measurements Using a Hybrid Significance Testing Approach by Hageman, Tyler S, Weis, David D

“…Differential hydrogen exchange-mass spectrometry (HX-MS) measurements are valuable for identification of differences in the higher order structures of…”
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Charge-mediated Fab-Fc interactions in an IgG1 antibody induce reversible self-association, cluster formation, and elevated viscosity by Arora, Jayant, Hu, Yue, Esfandiary, Reza, Sathish, Hasige A, Bishop, Steven M, Joshi, Sangeeta B, Middaugh, C Russell, Volkin, David B, Weis, David D

“…Concentration-dependent reversible self-association (RSA) of monoclonal antibodies (mAbs) poses a challenge to their pharmaceutical development as viable…”
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Characterization of Excipient Effects on Reversible Self-Association, Backbone Flexibility, and Solution Properties of an IgG1 Monoclonal Antibody at High Concentrations: Part 1 by Hu, Yue, Arora, Jayant, Joshi, Sangeeta B, Esfandiary, Reza, Middaugh, C Russell, Weis, David D, Volkin, David B

“…Many challenges limit the formulation of antibodies as high-concentration liquid dosage forms including elevated solution viscosity, decreased physical…”
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Effects of Salts from the Hofmeister Series on the Conformational Stability, Aggregation Propensity, and Local Flexibility of an IgG1 Monoclonal Antibody by Majumdar, Ranajoy, Manikwar, Prakash, Hickey, John M, Samra, Hardeep S, Sathish, Hasige A, Bishop, Steven M, Middaugh, C. Russell, Volkin, David B, Weis, David D

“…This work examines the effect of three anions from the Hofmeister series (sulfate, chloride, and thiocyanate) on the conformational stability and aggregation…”
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Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry by More, Apurva S, Toth, 4th, Ronald T, Okbazghi, Solomon Z, Middaugh, C Russell, Joshi, Sangeeta B, Tolbert, Thomas J, Volkin, David B, Weis, David D

“…We have used hydrogen exchange-mass spectrometry to characterize local backbone flexibility of 4 well-defined IgG1-Fc glycoforms expressed and purified from…”
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Structural Basis for Activation of Calcineurin by Calmodulin by Rumi-Masante, Julie, Rusinga, Farai I., Lester, Terrence E., Dunlap, Tori B., Williams, Todd D., Dunker, A. Keith, Weis, David D., Creamer, Trevor P.

“…The highly conserved phosphatase calcineurin (CaN) plays vital roles in numerous processes including T-cell activation, development and function of the central…”
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Correlations between changes in conformational dynamics and physical stability in a mutant IgG1 mAb engineered for extended serum half-life by Majumdar, Ranajoy, Esfandiary, Reza, Bishop, Steven M, Samra, Hardeep S, Middaugh, C Russell, Volkin, David B, Weis, David D

“…This study compares the local conformational dynamics and physical stability of an IgG1 mAb (mAb-A) with its corresponding YTE (M255Y/S257T/T259E) mutant…”
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Structural Elucidation of a Protective B Cell Epitope on Outer Surface Protein C (OspC) of the Lyme Disease Spirochete, Borreliella burgdorferi by Rudolph, Michael J, Davis, Simon A, Haque, H M Emranul, Weis, David D, Vance, David J, Piazza, Carol Lyn, Ejemel, Monir, Cavacini, Lisa, Wang, Yang, Mbow, M Lamine, Gilmore, Robert D, Mantis, Nicholas J

“…Outer surface protein C (OspC) plays a pivotal role in mediating tick-to-host transmission and infectivity of the Lyme disease spirochete, Borreliella…”
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Identification and Characterization of EX1 Kinetics in H/D Exchange Mass Spectrometry by Peak Width Analysis by Weis, David D., Wales, Thomas E., Engen, John R., Hotchko, Matthew, Ten Eyck, Lynn F.

“…Proteins that undergo cooperative unfolding events display EX1 kinetic signatures in hydrogen exchange mass spectra. The hallmark bimodal isotope pattern…”
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High-throughput biophysical analysis and data visualization of conformational stability of an IgG1 monoclonal antibody after deglycosylation by Alsenaidy, Mohammad A, Kim, Jae Hyun, Majumdar, Ranajoy, Weis, David D, Joshi, Sangeeta B, Tolbert, Thomas J, Middaugh, C Russell, Volkin, David B

“…The structural integrity and conformational stability of an IgG1 monoclonal antibody (mAb), after partial or complete enzymatic removal of the N-linked Fc…”
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Comment on Houde, D.; Berkowitz, S. A.; Engen, J. R., The Utility of Hydrogen/Deuterium Exchange Mass Spectrometry in Biopharmaceutical Comparability Studies. J. Pharm. Sci. 2011, 100, 2071-2086 by Weis, David D

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Analysis of Overlapped and Noisy Hydrogen/Deuterium Exchange Mass Spectra by Guttman, Miklos, Weis, David D., Engen, John R., Lee, Kelly K.

“…Noisy and overlapped mass spectrometry data hinder the sequence coverage that can be obtained from hydrogen deuterium exchange analysis, and places a limit on…”
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Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments by Masson, Glenn R., Burke, John E., Ahn, Natalie G., Anand, Ganesh S., Borchers, Christoph, Brier, Sébastien, Bou-Assaf, George M., Engen, John R., Englander, S. Walter, Faber, Johan, Garlish, Rachel, Griffin, Patrick R., Gross, Michael L., Guttman, Miklos, Hamuro, Yoshitomo, Heck, Albert J. R., Houde, Damian, Iacob, Roxana E., Jørgensen, Thomas J. D., Kaltashov, Igor A., Klinman, Judith P., Konermann, Lars, Man, Petr, Mayne, Leland, Pascal, Bruce D., Reichmann, Dana, Skehel, Mark, Snijder, Joost, Strutzenberg, Timothy S., Underbakke, Eric S., Wagner, Cornelia, Wales, Thomas E., Walters, Benjamin T., Weis, David D., Wilson, Derek J., Wintrode, Patrick L., Zhang, Zhongqi, Zheng, Jie, Schriemer, David C., Rand, Kasper D.

“…Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the…”
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Minimizing Carry-Over in an Online Pepsin Digestion System used for the H/D Exchange Mass Spectrometric Analysis of an IgG1 Monoclonal Antibody by MAJUMDAR, Ranajoy, MANIKWAR, Prakash, HICKEY, John M, ARORA, Jayant, RUSSELL MIDDAUGH, C, VOLKIN, David B, WEIS, David D

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High-Throughput Epitope Mapping by Hydrogen Exchange-Mass Spectrometry by Haque, H. M. Emranul, Mantis, Nicholas J., Weis, David D.

“…In this paper, we introduce a screening protocol for epitope mapping by hydrogen exchange mass spectrometry (HX-MS) that has higher throughput than a…”
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A Collection of Single-Domain Antibodies that Crowd Ricin Toxin's Active Site by Angalakurthi, Siva Krishna, Vance, David J, Rong, Yinghui, Nguyen, Chi My Thi, Rudolph, Michael J, Volkin, David, Middaugh, C Russell, Weis, David D, Mantis, Nicholas J

“…In this report, we used hydrogen exchange-mass spectrometry (HX-MS) to identify the epitopes recognized by 21 single-domain camelid antibodies (V Hs) directed…”
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A Structural Variant Approach for Establishing a Detection Limit in Differential Hydrogen Exchange-Mass Spectrometry Measurements by Hageman, Tyler S, Weis, David D

“…Hydrogen exchange-mass spectrometry (HX-MS) is widely promoted for its ability to detect subtle perturbations in protein structure, but such perturbations will…”
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Mapping Residual Structure in Intrinsically Disordered Proteins at Residue Resolution Using Millisecond Hydrogen/Deuterium Exchange and Residue Averaging by Keppel, Theodore R., Weis, David D.

“…Measurement of residual structure in intrinsically disordered proteins can provide insights into the mechanisms by which such proteins undergo coupled binding…”
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Understanding the Effects of Site-Specific Light Chain Conjugation on Antibody Structure Using Hydrogen Exchange-Mass Spectrometry (HX-MS) by Karunaratne, Sachini P, Moussa, Ehab M, Mills, Brittney J, Weis, David D

“…Antibody drug conjugates (ADCs) represent one of the fastest growing classes of cancer therapeutics. Drug incorporation through site-specific conjugation in…”
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