Expression profiling of circulating lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 in lung cancer patients

Long noncoding RNAs (lncRNAs) are crucial regulators of biological processes such as transcription interference and activation, chromatin remodeling, and mRNA translation. Uncontrolled gene expression could result from various epigenetic modifiers, like lncRNAs. So, this study aimed to evaluate the...

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Published in:	BMC cancer Vol. 24; no. 1; pp. 1175 - 12
Main Authors:	Hussein, Neveen A, Ebied, Samia A, Belal, Abdel Aziz M, Ahmad, Mohamad A, Weheida, El Sayed A
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 23-09-2024 BioMed Central BMC
Subjects:	Adult Aged Analysis Apoptosis Biological markers Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Bladder cancer Blood levels Chromatin remodeling Development and progression Diagnosis Down-regulation Epigenetics Family medical history Female Gene expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Genetic aspects Genetic engineering Humans Immunoassay Inflammatory diseases Kaplan-Meier Estimate lncRNA AATBC lncRNA GIAT4RA lncRNA Sirt1-AS Lung cancer Lung diseases Lung Neoplasms - blood Lung Neoplasms - genetics Lung Neoplasms - mortality Lung Neoplasms - pathology Lymphatic system Male Metastasis Middle Aged Non-coding RNA Patients Phosphopyruvate hydratase Prognosis Risk factors RNA, Long Noncoding - blood RNA, Long Noncoding - genetics SIRT1 protein Sirtuin 1 - blood Sirtuin 1 - genetics SMARCB1 SMARCB1 Protein - genetics Thermal cycling Transcription activation Egypt lncRNA Sirt1-AS SMARCB1 lncRNA GIAT4RA Lung cancer lncRNA AATBC
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Summary:	Long noncoding RNAs (lncRNAs) are crucial regulators of biological processes such as transcription interference and activation, chromatin remodeling, and mRNA translation. Uncontrolled gene expression could result from various epigenetic modifiers, like lncRNAs. So, this study aimed to evaluate the expression profiles of lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 in lung cancer. The current study included lung cancer patients (n = 50), patients with chronic inflammatory diseases (n = 30), and healthy volunteers (n = 20). The expression of blood genes and the concentration of serum neuron-specific enolase were determined by real-time PCR and electrochemiluminescence immunoassay, respectively. The receiver operating characteristic and Kaplan-Meier analyses assess the sensitivity of genes as diagnostic and prognostic biomarkers, respectively. LncRNA GIAT4RA and lncRNA AATBC were upregulated, while lncRNA Sirt1-AS was significantly downregulated in all patients compared to the control group. SMARCB1 expression was significantly downregulated in chronic inflammatory patients, while in those with lung cancer, it showed an insignificant difference. The expression of lncRNA GIAT4RA and lncRNA AATBC was significantly related to the stage of lung cancer. The survival analyses showed that lower lncRNA Sirt1-AS was linked to lung cancer patients' poorer disease-free survival and overall survival. Differences in lncRNA GIAT4RA, lncRNA AATBC, and lncRNA Sirt1-AS expression were detected in all patients. The consequent abnormal expression of lncRNAs could be crucial in lung cancer development. LncRNA GIAT4RA, lncRNA AATBC, and lncRNA Sirt1-AS may be utilized as promising diagnostic biomarkers. LncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 may be valuable prognostic biomarkers for lung cancer.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2407 1471-2407
DOI:	10.1186/s12885-024-12896-1