Search Results - "Weeks, K M"

van't Hoff enthalpies without baselines by JOHN, DEBORAH M., WEEKS, KEVIN M.

“…Analysis of thermal melting curves represents one important approach for evaluating protein stability and the consequences of amino acid substitution on…”
Get full text

RNA recognition by Tat-derived peptides: interaction in the major groove? by Weeks, K M, Crothers, D M

“…Replication of human immunodeficiency virus requires binding of the viral Tat protein to its RNA target sequence TAR; peptides derived from Tat bind to a TAR…”
Get more information

Visualizing induced fit in early assembly of the human signal recognition particle by Weeks, Kevin M, Rose, Marsha A

“…Assembly of almost all ribonucleoprotein complexes involves induced fit in the RNA and, thus, formation of one or more intermediate states. In assembly of the…”
Get full text

A collapsed state functions to self-chaperone RNA folding into a native ribonucleoprotein complex by Weeks, Kevin M, Webb, Amy E

“…Most large RNAs achieve their active, native structures only as complexes with one or more cofactor proteins. By varying the Mg2+ concentration, the catalytic…”
Get full text

Major Groove Accessibility of RNA by Weeks, Kevin M., Crothers, Donald M.

“…Chemical acylation experiments showed that the RNA major groove, often assumed to be too deep and narrow to permit recognition interactions, is accessible at…”
Get full text

RNA binding assays for Tat-derived peptides: implications for specificity by Weeks, Kevin M, Crothers, Donald M

“…RNA recognition by the HIV Tat protein is mediated in part by an arginine- and lysine-rich basic subdomain implicated as a signature element in proteins that…”
Get full text

Frequency of the codon 807 mutation in the cGMP phosphodiesterase β-subunit gene in Irish setters and other dog breeds with hereditary retinal degeneration by Aguirre, GD, Baldwin, V, Weeks, KM, Acland, GM, Ray, K

“…Rod-cone dysplasia 1 (rcd1) in Irish setters is caused by a nonsense mutation in the cGMP phosphodiesterase β-subunit gene (PDE6B). We examined the frequency…”
Get full text

Protein-facilitated RNA folding by Weeks, Kevin M

“…In the absence of protein collaborators, both simple and complex RNAs often misfold or are unfolded. Biologically important RNAs solve their folding problem,…”
Get full text

RNA-Tethered Phenyl Azide Photocrosslinking via a Short-Lived Indiscriminant Electrophile by Buchmueller, Karen L, Hill, Brian T, Platz, Matthew S, Weeks, Kevin M

“…Arylazide mediated photocrosslinking has been widely used to obtain structural constraints in biological systems, even though the reactive species generated…”
Get full text

Near Native Structure in an RNA Collapsed State by Buchmueller, Karen L., Weeks, Kevin M.

“…Many large RNAs form conformationally collapsed, but non-native, states prior to folding to the native state or assembling with protein cofactors. Although RNA…”
Get full text

A collapsed non-native RNA folding state by Weeks, Kevin M, Buchmueller, Karen L, Webb, Amy E, Richardson, D. Ashley

“…At physiological Mg2+ concentrations, the catalytic core of the bI5 group I intron does not fold into its native structure. In contrast, as judged by the…”
Get full text

Structural Basis for the Self-Chaperoning Function of an RNA Collapsed State by Garcia, Ivelitza, Weeks, Kevin M

“…Prior to folding to a native functional structure, many large RNAs form conformationally collapsed states. Formation of the near-native collapsed state for the…”
Get full text

Catalysis of Amide Synthesis by RNA Phosphodiester and Hydroxyl Groups by Chamberlin, Stacy I., Merino, Edward J., Weeks, Kevin M.

“…The functional groups found among the RNA bases and in the phosphoribose backbone represent a limited repertoire from which to construct a ribozyme active…”
Get full text

Protein-dependent transition states for ribonucleoprotein assembly by Webb, Amy E., Rose, Marsha A., Westhof, Eric, Weeks, Kevin M.

“…Native folding and splicing by the Saccharomyces cerevisiae mitochondrial bI5 group I intron RNA is facilitated by both the S. cerevisiae CBP2 and Neurospora…”
Get full text

Assembly of a Ribonucleoprotein Catalyst by Tertiary Structure Capture by Weeks, Kevin M., Cech, Thomas R.

“…CBP2 is an RNA tertiary structure binding protein required for efficient splicing of a yeast mitochondrial group I intron. CBP2 must wait for folding of the…”
Get full text

Differential Helix Stabilities and Sites Pre-organized for Tertiary Interactions Revealed by Monitoring Local Nucleotide Flexibility in the bI5 Group I Intron RNA by Chamberlin, Stacy I, Weeks, Kevin M

“…The local environment at adenosine residues in the bI5 group I intron RNA was monitored as a function of Mg2+ using both the traditional method of dimethyl…”
Get full text

Crystal Structures, Reactivity and Inferred Acylation Transition States for 2‘-Amine Substituted RNA by Gherghe, Costin M, Krahn, Joseph M, Weeks, Kevin M

“…Ribose 2‘-amine substitutions are broadly useful as structural probes in nucleic acids. In addition, structure-selective chemical reaction at 2‘-amine groups…”
Get full text

QuShape: rapid, accurate, and best-practices quantification of nucleic acid probing information, resolved by capillary electrophoresis by Karabiber, Fethullah, McGinnis, Jennifer L, Favorov, Oleg V, Weeks, Kevin M

“…Chemical probing of RNA and DNA structure is a widely used and highly informative approach for examining nucleic acid structure and for evaluating interactions…”
Get full text

Fragments of the HIV-1 Tat Protein Specifically Bind TAR RNA by Weeks, Kevin M., Ampe, Christophe, Schultz, Steve C., Steitz, Thomas A., Crothers, Donald M.

“…Proteolytically produced carboxyl-terminal fragments of the human immunodeficiency virus type-1 (HIV-1) Tat protein that include a conserved region rich in…”
Get full text

Protein facilitation of group I intron splicing by assembly of the catalytic core and the 5′ splice site domain by Weeks, Kevin M, Cech, Thomas R

“…The yeast mitochondrial group I intron bl5 undergoes self-splicing at high Mg 2+ concentrations, but requires the splicing factor CBP2 for reaction under…”
Get full text