Search Results - "Weeks, Donald P."

Efficient CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana and inheritance of modified genes in the T2 and T3 generations by Jiang, WenZhi, Yang, Bing, Weeks, Donald P

“…The newly developed CRISPR/Cas9 system for targeted gene knockout or editing has recently been shown to function in plants in both transient expression systems…”
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Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice by Jiang, Wenzhi, Zhou, Huanbin, Bi, Honghao, Fromm, Michael, Yang, Bing, Weeks, Donald P

“…The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new…”
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Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice by Zhou, Huanbin, Liu, Bo, Weeks, Donald P, Spalding, Martin H, Yang, Bing

“…The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for targeted gene editing in various organisms, including plants, where…”
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High-efficiency TALEN-based gene editing produces disease-resistant rice by Li, Ting, Liu, Bo, Spalding, Martin H, Weeks, Donald P, Yang, Bing

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Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii by Jiang, Wenzhi, Brueggeman, Andrew J, Horken, Kempton M, Plucinak, Thomas M, Weeks, Donald P

“…The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a powerful and precise tool for targeted gene modification (e.g.,…”
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Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing by Noel, Eric A, Weeks, Donald P, Van Etten, James L

“…Genetic and molecular modifications of the large dsDNA chloroviruses, with genomes of 290 to 370 kb, would expedite studies to elucidate the functions of both…”
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Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii by Rasala, Beth A., Barrera, Daniel J., Ng, Jenny, Plucinak, Thomas M., Rosenberg, Julian N., Weeks, Donald P., Oyler, George A., Peterson, Todd C., Haerizadeh, Farzad, Mayfield, Stephen P.

“…Summary Fluorescent proteins (FPs) have become essential tools for a growing number of fields in biology. However, such tools have not been widely adopted for…”
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Homologous recombination in Nannochloropsis: A powerful tool in an industrially relevant alga by Weeks, Donald P.

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Improved and versatile viral 2A platforms for dependable and inducible high‐level expression of dicistronic nuclear genes in Chlamydomonas reinhardtii by Plucinak, Thomas M, Horken, Kempton M, Jiang, Wenzhi, Fostvedt, Jessica, Nguyen, Sanh Tan, Weeks, Donald P

“…A significantly improved viral 2A peptide system for dependable high‐level expression of dicistronic genes in Chlamydomonas reinhardtii has been developed…”
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Evaluation of three herbicide resistance genes for use in genetic transformations and for potential crop protection in algae production by Brueggeman, Andrew J, Kuehler, Daniel, Weeks, Donald P

“…Genes conferring resistance to the herbicides glyphosate, oxyfluorfen and norflurazon were developed and tested for use as dominant selectable markers in…”
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Knockdown of limiting-CO2–induced gene HLA3 decreases HCO3− transport and photosynthetic Ci affinity in Chlamydomonas reinhardtii by Duanmu, Deqiang, Miller, Amy R, Horken, Kempton M, Weeks, Donald P, Spalding, Martin H

“…The CO 2 -concentrating mechanism (CCM) of Chlamydomonas reinhardtii and other microalgal species is essential for photosynthetic growth in most natural…”
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Use of designer nucleases for targeted gene and genome editing in plants by Weeks, Donald P, Spalding, Martin H, Yang, Bing

“…The ability to efficiently inactivate or replace genes in model organisms allowed a rapid expansion of our understanding of many of the genetic, biochemical,…”
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Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus by Weeks, Donald P

“…Polyploid crops make up a significant portion of the major food and fiber crops of the world and include wheat, potato, cotton, apple, peanut, citrus, and…”
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Enhancing soybean photosynthetic CO 2 assimilation using a cyanobacterial membrane protein, ictB by Hay, William T, Bihmidine, Saadia, Mutlu, Nedim, Hoang, Khang Le, Awada, Tala, Weeks, Donald P, Clemente, Tom E, Long, Stephen P

“…Soybean C photosynthesis can suffer a severe loss in efficiency due to photorespiration and the lack of a carbon concentrating mechanism (CCM) such as those…”
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Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing by Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., Weeks, Donald P.

“…Summary The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly…”
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TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain by Li, Ting, Huang, Sheng, Jiang, Wen Zhi, Wright, David, Spalding, Martin H, Weeks, Donald P, Yang, Bing

“…DNA double-strand breaks enhance homologous recombination in cells and have been exploited for targeted genome editing through use of engineered endonucleases…”
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Psr1, a Nuclear Localized Protein That Regulates Phosphorus Metabolism in Chlamydomonas by Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke

“…Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing…”
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The Cia5 Gene Controls Formation of the Carbon Concentrating Mechanism in Chlamydomonas reinhardtii by Xiang, Youbin, Zhang, Jun, Weeks, Donald P.

“…Wild-type Chlamydomonas reinhardtii cells shifted from high concentrations (5%) of CO2to low, ambient levels (0.03%) rapidly increase transcription of mRNAs…”
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Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing by Eric A Noel, Donald P Weeks, James L Van Etten

“…Genetic and molecular modifications of the large dsDNA chloroviruses, with genomes of 290 to 370 kb, would expedite studies to elucidate the functions of both…”
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Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes by Li, Ting, Huang, Sheng, Zhao, Xuefeng, Wright, David A., Carpenter, Susan, Spalding, Martin H., Weeks, Donald P., Yang, Bing

“…Recent studies indicate that the DNA recognition domain of transcription activator-like (TAL) effectors can be combined with the nuclease domain of FokI…”
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