Search Results - "Weeks, Amy M."

Redox-based reagents for chemoselective methionine bioconjugation by Lin, Shixian, Yang, Xiaoyu, Jia, Shang, Weeks, Amy M., Hornsby, Michael, Lee, Peter S., Nichiporuk, Rita V., Iavarone, Anthony T., Wells, James A., Toste, F. Dean, Chang, Christopher J.

“…Cysteine can be specifically functionalized by a myriad of acid-base conjugation strategies for applications ranging from probing protein function to…”
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Catalytic control of enzymatic fluorine specificity by Weeks, Amy M, Chang, Michelle C. Y

“…The investigation of unique chemical phenotypes has led to the discovery of enzymes with interesting behaviors that allow us to explore unusual function. The…”
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Constructing de Novo Biosynthetic Pathways for Chemical Synthesis inside Living Cells by Weeks, Amy M, Chang, Michelle C. Y

“…Living organisms have evolved a vast array of catalytic functions that make them ideally suited for the production of medicinally and industrially relevant…”
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Subtiligase-Catalyzed Peptide Ligation by Weeks, Amy M, Wells, James A

“…Subtiligase-catalyzed peptide ligation is a powerful approach for site-specific protein bioconjugation, synthesis and semisynthesis of proteins and peptides,…”
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Kinases leave their mark on caspase substrates by Weeks, Amy M

“…Apoptosis is a cell death program that is executed by the caspases, a family of cysteine proteases that typically cleave after aspartate residues during a…”
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Engineering peptide ligase specificity by proteomic identification of ligation sites by Weeks, Amy M, Wells, James A

“…A comprehensive characterization of peptide ligase specificity using proteome-derived peptide libraries enables the identification of 72 new subtiligases and…”
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Spatially Resolved Tagging of Proteolytic Neo-N termini with Subtiligase-TM by Weeks, Amy M.

“…Mass spectrometry-based proteomics has been used successfully to identify substrates for proteases. Identification of protease substrates at the cell surface,…”
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Biochemical and Structural Characterization of the trans-Enoyl-CoA Reductase from Treponema denticola by Bond-Watts, Brooks B, Weeks, Amy M, Chang, Michelle C. Y

“…The production of fatty acids is an important cellular pathway for both cellular function and the development of engineered pathways for the synthesis of…”
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Protein engineering for selective proteomics by Radziwon, Katarzyna, Weeks, Amy M.

“…Post-translational modifications, complex formation, subcellular localization, and cell-type-specific expression create functionally distinct protein…”
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Mapping proteolytic neo-N termini at the surface of living cells by Weeks, Amy M., Byrnes, James R., Lui, Irene, Wells, James A.

“…N terminomics is a powerful strategy for profiling proteolytic neo-N termini, but its application to cell surface proteolysis has been limited by the low…”
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Mapping Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase by Amiridis, Aspasia A, Weeks, Amy M

“…N terminomics methods combine selective isolation of protein N-terminal peptides with mass spectrometry (MS)-based proteomics for global profiling of…”
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Engineered peptide ligases for cell signaling and bioconjugation by Frazier, Clara L, Weeks, Amy M

“…Enzymes that catalyze peptide ligation are powerful tools for site-specific protein bioconjugation and the study of cellular signaling. Peptide ligases can be…”
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Hypoxia Is a Dominant Remodeler of the Effector T Cell Surface Proteome Relative to Activation and Regulatory T Cell Suppression by Byrnes, James R., Weeks, Amy M., Shifrut, Eric, Carnevale, Julia, Kirkemo, Lisa, Ashworth, Alan, Marson, Alexander, Wells, James A.

“…Immunosuppressive factors in the tumor microenvironment (TME) impair T cell function and limit the antitumor immune response. T cell surface receptors and…”
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Entropy drives selective fluorine recognition in the fluoroacetyl–CoA thioesterase from Streptomyces cattleya by Weeks, Amy M., Wang, Ningkun, Pelton, Jeffrey G., Chang, Michelle C. Y.

“…Fluorinated small molecules play an important role in the design of bioactive compounds for a broad range of applications. As such, there is strong interest in…”
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Proteome-Derived Peptide Libraries for Deep Specificity Profiling of N-terminal Modification Reagents by Bridge, Haley N, Weeks, Amy M

“…Protein and peptide N termini are important targets for selective modification with chemoproteomics reagents and bioconjugation tools. The N-terminal ⍺-amine…”
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Direct Identification of Proteolytic Cleavages on Living Cells Using a Glycan-Tethered Peptide Ligase by Schaefer, Kaitlin, Lui, Irene, Byrnes, James R., Kang, Emily, Zhou, Jie, Weeks, Amy M., Wells, James A.

“…Proteolytic cleavage of cell surface proteins triggers critical processes including cell–cell interactions, receptor activation, and shedding of signaling…”
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Molecular Recognition of Fluorine Impacts Substrate Selectivity in the Fluoroacetyl-CoA Thioesterase FlK by Weeks, Amy M, Keddie, Neil S, Wadoux, Rudy D. P, O’Hagan, David, Chang, Michelle C. Y

“…The fluoroacetate-producing bacterium Streptomyces cattleya has evolved a fluoroacetyl-CoA thioesterase (FlK) that exhibits a remarkably high level of…”
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Structural and Biochemical Studies of a Fluoroacetyl-CoA-Specific Thioesterase Reveal a Molecular Basis for Fluorine Selectivity by Weeks, Amy M, Coyle, Scott M, Jinek, Martin, Doudna, Jennifer A, Chang, Michelle C. Y

“…We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report…”
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Temporal and Fluoride Control of Secondary Metabolism Regulates Cellular Organofluorine Biosynthesis by Walker, Mark C, Wen, Miao, Weeks, Amy M, Chang, Michelle C. Y

“…Elucidating mechanisms of natural organofluorine biosynthesis is essential for a basic understanding of fluorine biochemistry in living systems as well as for…”
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N-Terminal Modification of Proteins with Subtiligase Specificity Variants by Weeks, Amy M, Wells, James A

“…Subtiligase is a powerful enzymatic tool for N-terminal modification of proteins and peptides. In a typical subtiligase-catalyzed N-terminal modification…”
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