Search Results - "Webb, Bradley A."

Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations by Webb, Bradley A., Forouhar, Farhad, Szu, Fu-En, Seetharaman, Jayaraman, Tong, Liang, Barber, Diane L.

“…The first structures of the mammalian phosphofructokinase-1 tetramer are reported, for the human platelet isoform, in complex with ATP–Mg 2+ and ADP. Key…”
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Considering Protonation as a Posttranslational Modification Regulating Protein Structure and Function by Schönichen, André, Webb, Bradley A, Jacobson, Matthew P, Barber, Diane L

“…Posttranslational modification is an evolutionarily conserved mechanism for regulating protein activity, binding affinity, and stability. Compared with…”
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Structural basis for allosteric regulation of human phosphofructokinase-1 by Lynch, Eric M., Hansen, Heather, Salay, Lauren, Cooper, Madison, Timr, Stepan, Kollman, Justin M., Webb, Bradley A.

“…Phosphofructokinase-1 (PFK1) catalyzes the rate-limiting step of glycolysis, committing glucose to conversion into cellular energy. PFK1 is highly regulated to…”
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pH sensing by FAK-His58 regulates focal adhesion remodeling by Choi, Chang-Hoon, Webb, Bradley A, Chimenti, Michael S, Jacobson, Matthew P, Barber, Diane L

“…Intracellular pH (pHi) dynamics regulates diverse cellular processes, including remodeling of focal adhesions. We now report that focal adhesion kinase (FAK),…”
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The Sodium-Hydrogen Exchanger NHE1 Is an Akt Substrate Necessary for Actin Filament Reorganization by Growth Factors by Meima, Marcel E., Webb, Bradley A., Witkowska, H.Ewa, Barber, Diane L.

“…The kinase Akt mediates signals from growth factor receptors for increased cell proliferation, survival, and migration, which contribute to the positive…”
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Dysregulated pH: a perfect storm for cancer progression by Barber, Diane L, Webb, Bradley A, Chimenti, Michael, Jacobson, Matthew P

“…Dysregulated pH is emerging as a hallmark of cancer because cancers have a 'reversed' pH gradient, with a constitutively increased intracellular pH that is…”
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Caldesmon is an integral component of podosomes in smooth muscle cells by Eves, Robert, Webb, Bradley A, Zhou, Shutang, Mak, Alan S

“…Podosomes are highly dynamic actin-based structures commonly found in motile and invasive cells such as macrophages, osteoclasts and vascular smooth muscle…”
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Selective activation of PFKL suppresses the phagocytic oxidative burst by Amara, Neri, Cooper, Madison P., Voronkova, Maria A., Webb, Bradley A., Lynch, Eric M., Kollman, Justin M., Ma, Taylur, Yu, Kebing, Lai, Zijuan, Sangaraju, Dewakar, Kayagaki, Nobuhiko, Newton, Kim, Bogyo, Matthew, Staben, Steven T., Dixit, Vishva M.

“…In neutrophils, nicotinamide adenine dinucleotide phosphate (NADPH) generated via the pentose phosphate pathway fuels NADPH oxidase NOX2 to produce reactive…”
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Filament formation by metabolic enzymes—A new twist on regulation by Lynch, Eric M., Kollman, Justin M., Webb, Bradley A.

“…Compartmentalization of metabolic enzymes through protein–protein interactions is an emerging mechanism for localizing and regulating metabolic activity…”
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The glycolytic enzyme phosphofructokinase-1 assembles into filaments by Webb, Bradley A, Dosey, Anne M, Wittmann, Torsten, Kollman, Justin M, Barber, Diane L

“…Despite abundant knowledge of the regulation and biochemistry of glycolytic enzymes, we have limited understanding on how they are spatially organized in the…”
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pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells by Webb, Bradley A, Aloisio, Francesca M, Charafeddine, Rabab A, Cook, Jessica, Wittmann, Torsten, Barber, Diane L

“…Many lysosome functions are determined by a lumenal pH of ∼5.0, including the activity of resident acid-activated hydrolases. Lysosome pH (pHlys) is often…”
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A Histidine Cluster in the Cytoplasmic Domain of the Na-H Exchanger NHE1 Confers pH-sensitive Phospholipid Binding and Regulates Transporter Activity by Webb, Bradley A., White, Katharine A., Grillo-Hill, Bree K., Schönichen, André, Choi, Changhoon, Barber, Diane L.

“…The Na-H exchanger NHE1 contributes to intracellular pH (pHi) homeostasis in normal cells and the constitutively increased pHi in cancer. NHE1 activity is…”
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Phosphorylation of cortactin by p21-activated kinase by Webb, Bradley A., Zhou, Shutang, Eves, Robert, Shen, Linda, Jia, Lilly, Mak, Alan S.

“…Cortactin is an F-actin binding protein that is enriched in dynamic cytoskeletal organelles such as podosomes, membrane ruffles, and lamellipodia. We have…”
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A Ligand-induced Conformational Change in Apolipoprotein(a) Enhances Covalent Lp(a) Formation by Becker, Lev, Webb, Bradley A., Chitayat, Seth, Nesheim, Michael E., Koschinsky, Marlys L.

“…Lipoprotein(a) (Lp(a)) assembly proceeds via a two-step mechanism in which initial non-covalent interactions between apolipoprotein(a) (apo(a)) and low density…”
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Ratiometric Imaging of pH Probes by Grillo-Hill, Bree K., Webb, Bradley A., Barber, Diane L.

“…Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical…”
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Dissecting the functional domain requirements of cortactin in invadopodia formation by Webb, Bradley A., Jia, Lilly, Eves, Robert, Mak, Alan S.

“…Cells degrade extracellular matrix (ECM) barriers at focal locations by the formation of membrane protrusions called invadopodia. Polymerization of the actin…”
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Cortactin regulates podosome formation: Roles of the protein interaction domains by Webb, Bradley A., Eves, Robert, Mak, Alan S.

“…Cortactin, a multi-domain scaffolding protein involved in actin polymerization, is enriched in podosomes induced by phorbol ester in vascular smooth muscle…”
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A Role for Myosin-I in Actin Assembly through Interactions with Vrp1p, Bee1p, and the Arp2/3 Complex by Evangelista, Marie, Klebl, Bert M., Amy H. Y. Tong, Webb, Bradley A., Leeuw, Thomas, Leberer, Ekkehard, Whiteway, Malcolm, Thomas, David Y., Boone, Charles

“…Type I myosins are highly conserved actinbased molecular motors that localize to the actin-rich cortex and participate in motility functions such as…”
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PAK1 induces podosome formation in A7r5 vascular smooth muscle cells in a PAK-interacting exchange factor-dependent manner by Webb, Bradley A, Eves, Robert, Crawley, Scott W, Zhou, Shutang, Côté, Graham P, Mak, Alan S

“…Remodeling of the vascular smooth muscle cytoskeleton is essential for cell motility involved in the development of diseases such as arteriosclerosis and…”
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Effects of tyrosine phosphorylation of cortactin on podosome formation in A7r5 vascular smooth muscle cells by Zhou, Shutang, Webb, Bradley A, Eves, Robert, Mak, Alan S

“…Cortactin, a predominant substrate of Src family kinases, plays an important role in Arp2/3-dependent actin polymerization in lamellipodia and membrane ruffles…”
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