Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties

•Quantitative determination of bioactive sinapine in rapeseed pomace (RSP) extract.•Anti-oxidant/radical scavenging properties of sinapine at RSP extract concentration.•DNA protective properties of sinapine against free radical inducer.•Sinapine inhibited AChE, a target for treatment of neurodegener...

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Bibliographic Details
Published in:Food chemistry Vol. 276; pp. 768 - 775
Main Authors: Yates, Kyari, Pohl, Franziska, Busch, Maike, Mozer, Annika, Watters, Louis, Shiryaev, Andrey, Kong Thoo Lin, Paul
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15-03-2019
Subjects:
Acetylcholinesterase (AChE)
Acetylcholinesterase - chemistry
Acetylcholinesterase - metabolism
Antioxidant assays
Antioxidants - analysis
Antioxidants - pharmacology
Brassica rapa - chemistry
Canola
Choline - analogs & derivatives
Choline - analysis
Choline - chemistry
Choline - pharmacology
Cholinesterase Inhibitors - analysis
Cholinesterase Inhibitors - pharmacology
Chromatography, Liquid
Gallic Acid - analogs & derivatives
Gallic Acid - analysis
LC-MS/MS
Molecular Docking Simulation
Phenolic acids
Phenols - analysis
Plant Extracts - analysis
Plant Extracts - chemistry
Plasmid DNA (pBR322)
Rapeseed pomace
Scotland
Sinapine
Tandem Mass Spectrometry
Scotland
Plasmid DNA (pBR322)
LC-MS/MS
Acetylcholinesterase (AChE)
Sinapine
Rapeseed pomace
Canola
Antioxidant assays
Phenolic acids
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Summary:•Quantitative determination of bioactive sinapine in rapeseed pomace (RSP) extract.•Anti-oxidant/radical scavenging properties of sinapine at RSP extract concentration.•DNA protective properties of sinapine against free radical inducer.•Sinapine inhibited AChE, a target for treatment of neurodegenerative diseases.•RSP is a source of bioactive compounds for nutraceutical & pharmaceutical industry. Sinapine is the main secondary metabolite present in rapeseed pomace (RSP) with its concentration being dependent on rapeseed processing, growing conditions, extraction parameters and the country of origin. Here we report, the concentration of sinapine from an extract of defatted RSP harvested in the North East of Scotland. Using liquid chromatography tandem mass spectrometry, the most abundant phenolic compound in the RSP extract was, as expected, sinapine (109.1 mg/g RSP extract). Additionally, sinapic, caffeic, ferulic and syringic acids were identified (0.159–3.91 mg/g RSP extract). Sinapine together with the phenolics at the concentration present in the RSP extract, exhibited ≥50% activity relative to the extract in antioxidant assays. Furthermore, sinapine provided plasmid DNA (pBR322) protection, from 2,2′-azobis(2-amidinopropane) dihydrochloride and inhibited acetylcholinesterase activity by 85%. Molecular docking was utilised to explain the inhibitory activity. RSP can be an excellent source of bioactive compounds for pharmaceuticals, food additive and nutraceutical applications.
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ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2018.10.045