Search Results - "Watson, J Throck"

Detection of Phosphopeptides Using Fe(III)−Nitrilotriacetate Complexes Immobilized on a MALDI Plate by Dunn, Jamie D, Watson, J. Throck, Bruening, Merlin L

“…Metal affinity complexes were chemically grafted onto the surface of gold matrix-assisted laser desorption/ionization (MALDI) plates by coupling a derivative…”
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Non-specific, on-probe cleanup methods for MALDI-MS samples by Xu, Yingda, Bruening, Merlin L., Watson, J. Throck

“…I. Introduction 429  II. Decontamination with Films of Commercial Polymers 431 A.  Polyvinylidenedifluoride and Nitrocellulose 431 B.  Nylon 431 C.  Nafion 432…”
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Patterned Monolayer/Polymer Films for Analysis of Dilute or Salt-Contaminated Protein Samples by MALDI-MS by Xu, Yingda, Watson, J. Throck, Bruening, Merlin L.

“…This paper describes a surface science/mass spectrometry effort to develop and characterize a patterned gold surface that serves as a MALDI sample platform…”
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Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology by Gallegos-Pérez, José-Luis, Rangel-Ordóñez, Laura, Bowman, Stephen Robert, Ngowe, Charles O., Watson, J. Throck

“…In a study of primary (methyl to butyl) amines as nucleophiles for cyano-induced cleavage of cysteinyl proteins, methylamine was found to be superior to…”
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Mass spectrometric evidence for mechanisms of fragmentation of charge-derivatized peptides by Sadagopan, Nalini, Watson, J.Throck

“…Mass spectrometry of charged derivatives of peptides has been a growing area of interest in the past decade. Fragmentation of charged derivatives of peptides…”
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Investigation of the tris(trimethoxyphenyl)phosphonium acetyl charged derivatives of peptides by electrospray ionization mass spectrometry and tandem mass spectrometry by Sadagopan, Nalini, Watson, J.Throck

“…Charged derivatives of peptides are useful in obtaining simpler collision-activated dissociation (CAD) mass spectra. An N-terminal charge-derivatizing reagent…”
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Automated data interpretation based on the concept of “negative signature mass” for mass-mapping disulfide structures of cystinyl proteins by Qi, Jianfeng, Wu, Wei, Borges, Chad R, Hang, Dehua, Rupp, Matthew, Torng, Eric, Watson, J.Throck

“…An efficient method for data processing and interpretation is needed to support and extend disulfide mass-mapping methodology based on partial reduction and…”
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Trapping of intermediates during the refolding of recombinant human epidermal growth factor (hEGF) by cyanylation, and subsequent structural elucidation by mass spectrometry by Wu, Jiang, YingYang, Throck Watson, J.

“…Human epidermal growth factor (hEGF) contains 53 amino acids and three disulfide bonds. The unfolded, reduced hEGF is allowed to refold under mildly alkaline…”
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Use of Polymer-Modified MALDI-MS Probes To Improve Analyses of Protein Digests and DNA by Xu, Yingda, Bruening, Merlin L, Watson, J. Throck

“…The use of sample probe surfaces patterned with 200-μm-diameter spots of hydrophilic, charged polymers significantly enhances the analysis of protein digests…”
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Peroxynitrite-induced nitration of tyrosine hydroxylase: identification of tyrosines 423, 428, and 432 as sites of modification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and tyrosine-scanning mutagenesis by Kuhn, Donald M, Sadidi, Mahdieh, Liu, Xiuli, Kreipke, Christian, Geddes, Timothy, Borges, Chad, Watson, J Throck

“…Tyrosine hydroxylase (TH), the initial and rate-limiting enzyme in the biosynthesis of the neurotransmitter dopamine, is inactivated by peroxynitrite. The…”
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Assignment of disulfide bonds in proteins by chemical cleavage and peptide mapping by mass spectrometry by Wu, Jiang, Watson, J Throck

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Integration of hydrogen/deuterium exchange and cyanylation-based methodology for conformational studies of cystinyl proteins by Li, Xue, Chou, Yi-Te, Husain, Rhonda, Watson, J Throck

“…This report documents the feasibility and advantages of integrating hydrogen/deuterium exchange (HDX) methodology with cyanylation (CN)-based methodology to…”
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Separation and quantitation of linoleic acid oxidation products in mammary gland tissue from mice fed low- and high-fat diets by Johnson, J.A. (Michigan State University, East Lansing, MI.), Blackburn, M.L, Bull, A.W, Welsch, C.W, Watson, J.T

“…We have developed an assay for the isolation and quantitation by gas chromatography‐mass spectrometry (GC‐MS) of free 9‐ and 13‐hydroxyoctadecadienoic acid…”
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Recognition of cysteine‐containing peptides through prompt fragmentation of the 4‐dimethylaminophenylazophenyl‐4′‐maleimide derivative during analysis by MALDI‐MS by Borges, Chad R., Watson, J. Throck

“…Under specified UV‐MALDI conditions, the 4‐dimethylaminophenylazophenyl‐4'‐maleimide (DABMI) derivative of cysteine‐containing peptides undergoes prompt…”
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A Picomole-Scale Method for Charge Derivatization of Peptides for Sequence Analysis by Mass Spectrometry by Huang, Zhi-Heng, Wu, Jiang, Roth, Kenneth D. W, Yang, Ying, Gage, Douglas A, Watson, J. Throck

“…A highly activated ester containing a fixed positive charge, S-pentafluorophenyl [tris(2,4,6-trimethoxyphenyl)phosphonium]acetate bromide (TMPP-AcSC6F5…”
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Characterization of peptide folding nuclei by hydrogen/deuterium exchange‐mass spectrometry by Li, Xue, Hood, Robin J., Wedemeyer, William J., Watson, J. Throck

“…Covalently linked pairs of well‐chosen peptides can be good model systems for protein folding studies because they can adopt stable secondary, side‐chain, and…”
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Signature Sets', Minimal Fragment Sets for Identifying Protein Disulfide Structures with Cyanylation-Based Mass Mapping Methodology by Wu, Wei, Huang, Wei, Qi, Jianfeng, Chou, Yi-Te, Torng, Eric, Watson, J. Throck

“…Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods…”
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Selective detection of the tolyl cation among other [C 7H 7] + isomers by ion/molecule reaction with dimethyl ether by Heath, Timothy G., Allison, John, Throck Watson, J.

“…The ion/molecule reaction of the tolyl cation with dimethyl ether has been investigated using triple quadrupole mass spectrometry. Three isomers with [C 7H 7]…”
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Mass Mapping Sites of Nitration in Tyrosine Hydroxylase:  Random vs Selective Nitration of Three Tyrosine Residues by Borges, Chad R, Kuhn, Donald M, Watson, J. Throck

“…Previous work has shown that tyrosine residues Y423, Y428, and Y432 are potential targets for nitration when tyrosine hydroxylase is exposed to peroxynitrite…”
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Novel fragmentation process of peptides by collision-induced decomposition in a tandem mass spectrometer: differentiation of leucine and isoleucine by Johnson, Richard S, Martin, Stephen A, Biemann, Klaus, Stults, John T, Watson, J. Throck

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