Rapid microwave-stimulated fixation of fine-needle aspiration biopsies for transmission electron microscopy

A new technique is described in which fine-needle aspiration biopsies are processed for transmission electron microscopy within 2 hr. This rapid processing method includes exposure of the biopsy in a glutaraldehyde-formaldehyde mixture to microwaves for 25 sec in a domestic oven. The specimen is the...

Full description

Saved in:

Bibliographic Details
Published in:	Diagnostic cytopathology Vol. 6; no. 1; p. 68
Main Authors:	Gove, D W, Lang, C A, Waterhouse, L K, Leong, A S
Format:	Journal Article
Language:	English
Published:	United States 1990
Subjects:	Biopsy, Needle - methods Carcinoma - pathology Fixatives Humans Lung Neoplasms - pathology Microscopy, Electron - methods Microwaves
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A new technique is described in which fine-needle aspiration biopsies are processed for transmission electron microscopy within 2 hr. This rapid processing method includes exposure of the biopsy in a glutaraldehyde-formaldehyde mixture to microwaves for 25 sec in a domestic oven. The specimen is then routinely stained, processed through graded ethanol, and embedded in epoxy resin to produce excellent preservation of fine structural features.
ISSN:	8755-1039
DOI:	10.1002/dc.2840060115