Search Results - "Warrior, Usha"

Evaluation of the use of imaging parameters for the detection of compound-induced hepatotoxicity in 384-well cultures of HepG2 cells and cryopreserved primary human hepatocytes by Garside, Helen, Marcoe, Karen F., Chesnut-Speelman, Jessica, Foster, Alison J., Muthas, Daniel, Gerry Kenna, J., Warrior, Usha, Bowes, Joanne, Baumgartner, James

“…•Assessment of 108 DILI and 26 non-DILI drugs in 384-well plate assays.•Key parameters were measured in HepG2 cells ± rat S9 and primary human…”
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Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans by Abraham, Vivek C, Towne, Danli L, Waring, Jeffrey F, Warrior, Usha, Burns, David J

“…Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for…”
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chemical genomics screen highlights the essential role of mitochondria in HIF-1 regulation by Lin, Xiaoyu, David, Caroline A, Donnelly, Jennifer B, Michaelides, Mike, Chandel, Navdeep S, Huang, Xiaoli, Warrior, Usha, Weinberg, Frank, Tormos, Kathryn V, Fesik, Stephen W, Shen, Yu

“…Hypoxia-inducible factor-1 (HIF-1) plays an essential role in tumor development and progression by regulating genes that are vital for proliferation,…”
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Role of Rho kinase pathway in chondroitin sulfate proteoglycan-mediated inhibition of neurite outgrowth in PC12 cells by Gopalakrishnan, Sujatha M., Teusch, Nicole, Imhof, Christiane, Bakker, Margot H. M., Schurdak, Mark, Burns, David J., Warrior, Usha

“…Activation of the Rho kinase (ROCK) pathway has been associated with inhibition of neurite regeneration and outgrowth in spinal cord injury. Growth‐inhibitory…”
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Discovery of novel inhibitors of Bcl-xL using multiple high-throughput screening platforms by Qian, Jie, Voorbach, Martin J, Huth, Jeffrey R, Coen, Michael L, Zhang, Haichao, Ng, Shi-Chung, Comess, Kenneth M, Petros, Andrew M, Rosenberg, Saul H, Warrior, Usha, Burns, David J

“…Bcl-xL is a member of the Bcl-2 family of proteins that are implicated to play a vital role in several diseases including cancer. Bcl-xL suppresses apoptosis;…”
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Potent Inhibition of NFAT Activation and T Cell Cytokine Production by Novel Low Molecular Weight Pyrazole Compounds by Trevillyan, James M., Chiou, X. Grace, Chen, Yung-Wu, Ballaron, Stephen J., Sheets, Michael P., Smith, Morey L., Wiedeman, Paul E., Warrior, Usha, Wilkins, Julie, Gubbins, Earl J., Gagne, Gerard D., Fagerland, Jane, Carter, George W., Luly, Jay R., Mollison, Karl W., Djuric, Stevan W.

“…NFAT (nuclearfactor of activated T cell) proteins are expressed in most immune system cells and regulate the transcription of cytokine genes critical for the…”
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Utility of large-scale transiently transfected cells for cell-based high-throughput screens to identify transient receptor potential channel A1 (TRPA1) antagonists by Chen, Jun, Lake, Marc R, Sabet, Reza S, Niforatos, Wende, Pratt, Steve D, Cassar, Steven C, Xu, Jing, Gopalakrishnan, Sujatha, Pereda-Lopez, Ana, Gopalakrishnan, Murali, Holzman, Thomas F, Moreland, Robert B, Walter, Karl A, Faltynek, Connie R, Warrior, Usha, Scott, Victoria E

“…Despite increasing use of cell-based assays in high-throughput screening (HTS) and lead optimization, one challenge is the adequate supply of high-quality…”
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Validation of FLIPR membrane potential dye for high throughput screening of potassium channel modulators by Whiteaker, K L, Gopalakrishnan, S M, Groebe, D, Shieh, C C, Warrior, U, Burns, D J, Coghlan, M J, Scott, V E, Gopalakrishnan, M

“…A fluorescence-based assay using the FLIPR Membrane Potential Assay Kit (FMP) was evaluated for functional characterization and high throughput screening (HTS)…”
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Functional characterization of adenosine receptors and coupling to ATP-sensitive K+ channels in Guinea pig urinary bladder smooth muscle by Gopalakrishnan, Sujatha M, Buckner, Steven A, Milicic, Ivan, Groebe, Duncan R, Whiteaker, Kristi L, Burns, David J, Warrior, Usha, Gopalakrishnan, Murali

“…Although multiple adenosine receptors have been identified, the subtype and underlying mechanisms involved in the relaxation response to adenosine in the…”
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1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor by Chen, Zehan, Gopalakrishnan, Sujatha M, Bui, Mai-Ha, Soni, Niru B, Warrior, Usha, Johnson, Eric F, Donnelly, Jennifer B, Glaser, Keith B

“…Eukaryotic elongation factor-2 kinase (eEF2K) relays growth and stress signals to protein synthesis through phosphorylation and inactivation of eukaryotic…”
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Abstract B068: An in vitro cellular functional STING pathway assay to measure the potency of STING agonists, using interferon-β response by Sainathan, Satheesh K., Cracchiolo, Alyssa M., Warrior, Usha, King, Alastair J.

“…Abstract Introduction and Relevance: We have developed an in vitro cellular functional STING pathway assay that can be used to screen for potential therapeutic…”
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Abstract 1391: Establishment and validation of a high-content imaging assay with a 14-day incubation for the testing of epigenetic target-based therapeutics by Parry, Jesse J., Norman, Vanessa L., Wageman, Charles R., Cavedine, Lee R., Sindelar, Timothy J., Croff, Alyssa M., Garner, Steven M., Epkins, Brogan A., Robinson, Natiya E., Dempsey, Kristin C., Warrior, Usha, King, Alastair J.

“…Abstract Given the importance and significance of targeting mutations and epigenetic modification in cancer therapy, we sought to expand on our ability to test…”
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Abstract 2260: Exploring drug response metrics for biomarker discovery using the OncoPanel™ cell-based profiling service by Wageman, Charles R., Parry, Jesse J., Cavedine, Lee R., Croff, Alyssa M., Garner, Steven M., Epkins, Brogan A., Robinson, Natiya E., Dempsey, Kristin C., Norman, Vanessa L., Warrior, Usha, King, Alastair J.

“…Abstract Using drug response data, generated by the OncoPanelTM cell-based profiling service, we investigated the differences between traditional and…”
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Use of SAM2 biotin capture membrane in microarrayed compound screening (muARCS) format for nucleic acid polymerization assays by Xuei, Xiaoling, David, Caroline A, Middleton, Tim R, Lim, Ben, Pithawalla, Ron, Chen, Chih-Ming, Tripathi, Rakesh L, Burns, David J, Warrior, Usha

“…Microarrayed compound screening format (muARCS) is a novel high-throughput screening technology that uses agarose matrices to integrate various biochemical or…”
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Abstract 4259: Long term cell culture models to identify biomarkers of response by Warrior, Usha, Crane, Jonathan, Bernards, Karen, Nelson, Brian, Snead, Katie, Angione, Alison, Sherman, Amber, DeVault, Shawn, Hnilo, Jim, Shah, jameel

“…Abstract In order to test the efficacy of many cancer therapeutics, culturing cells in the presence of compounds for longer than the conventional 72-hr cell…”
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Abstract 2116: Combining the power of different profiling approaches to better understand the activity of kinase inhibitor drugs by King, Alastair J., Parry, Jesse J., Burkart, Samantha C., Cavedine, Lee R., Cracchiolo, Alyssa M., Epkins, Brogan A., Garner, Steven M., Sindelar, Timothy J., Wageman, Charles R., Davies, Stephen P., Plater, Andrew, Woodward, Anna M., Warrior, Usha

“…Abstract Profiling of inhibitors for eventual therapeutic use is an essential part of drug discovery and development. Evaluating the in vitro potency of kinase…”
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Abstract 5075: Phenotypic human tumor cell line profiling and predictive biomarker analysis for epigenetic inhibitors by Warrior, Usha, Parry, Jesse J., Burkart, Samantha C., Cavedine, Lee R., Cracchiolo, Alyssa M., Epkins, Brogan A., Garner, Steven M., Sindelar, Timothy J., Wageman, Charles R., King, Alastair J.

“…Abstract In recent years, epigenetic target inhibitors have emerged as viable therapeutics for treating various types of cancers. Testing of the epigenetic…”
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Abstract 5787: Validation of spheroids with long-term incubation for high-throughput screening by Parry, Jesse J., Garner, Steven M., Cavedine, Lee R., Cracchiolo, Alyssa M., Epkins, Brogan A., Burkart, Samantha C., Sindelar, Timothy J., Wageman, Charles R., Warrior, Usha, King, Alastair J.

“…Abstract The OncoPanel™ service has established 100 genomically-characterized human tumor cell lines as targets for the screening of compounds in 3D spheroid…”
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Abstract A127: Long-term cell culture models enable drug response determination for epigenetic therapeutics by Hnilo, Jim, Snead, Katie, Bernards, Karen, Nelson, Brian, McKinley, Keith, Waikins, Kate, Crane, Jonathan M., Warrior, Usha, Shah, O. Jameel

“…Abstract There has been renewed interest in the field of cancer epigenetics from the pharmaceutical and biotech sectors owing to the discovery of druggable…”
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Structure−Activity Studies on a Series of a 2-Aminopyrimidine-Containing Histamine H 4 Receptor Ligands by Altenbach, Robert J., Adair, Ronald M., Bettencourt, Brian M., Black, Lawrence A., Fix-Stenzel, Shannon R., Gopalakrishnan, Sujatha M., Hsieh, Gin C., Liu, Huaqing, Marsh, Kennan C., McPherson, Michael J., Milicic, Ivan, Miller, Thomas R., Vortherms, Timothy A., Warrior, Usha, Wetter, Jill M., Wishart, Neil, Witte, David G., Honore, Prisca, Esbenshade, Timothy A., Hancock, Arthur A., Brioni, Jorge D., Cowart, Marlon D.

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