Search Results - "Warren R.A.J"

The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose by Gilkes, N.R. (University of British Columbia, Vancouver, British Columbia, Canada), Jervis, E, Henrissat, B, Tekant, B, Miller, R.C. Jr, Warren, R.A.J, Kilburn, D.G

“…CenA is a bacterial cellulase (beta-1,4-glucanase) comprised of a globular catalytic domain joined to an extended cellulose-binding domain (CBD) by a short…”
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Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains by Langsford, M.L., Gilkes, N.R., Singh, B., Moser, B., Miller, R.C., Warren, R.A.J., Kilburn, D.G.

“…Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA…”
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Degradation of cellulases in cultures of Cellulomonas fimi by Sandercock, L.E, Meinke, A, Gilkes, N.R, Kilburn, D.G, Warren, R.A.J

“…Endoglucanases CenA, CenB and CenD, cellobiohydrolases CbhA and CbhB, and the mixed function xylanase-exoglucanase Cex are degraded proteolytically in the…”
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Structure of the gene encoding the exoglucanase of Cellulomonas fimi by O'Neill, G, Goh, S H, Warren, R A, Kilburn, D G, Miller, Jr, R C

“…In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose. The gene now has been sequenced as part of a 2.58-kb…”
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A scheme for designating enzymes that hydrolyse the polysaccharides in the cell walls of plants by Henrissat, B, Teeri, T.T, Warren, R.A.J

“…A scheme is proposed for designating enzymes that hydrolyse the polysaccharides in the cell walls of plants. These enzymes are predominantly β-1,4-glycanases…”
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Directed evolution of new glycosynthases from Agrobacterium β-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis by Mayer, Christoph, Jakeman, David L, Mah, Melanie, Karjala, Geoff, Gal, Laurent, Warren, R.A.J, Withers, Stephen G

“…Background: Oligosaccharide synthesis is becoming increasingly important to industry as diverse therapeutic roles for these molecules are discovered. The…”
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MICROBIAL HYDROLYSIS OF POLYSACCHARIDES by Warren, R. A. J

“…Microorganisms are efficient degraders of starch, chitin, and the polysaccharides in plant cell walls. Attempts to purify hydrolases led to the realization…”
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The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum by Paradis, F W, Warren, R A, Kilburn, D G, Miller, Jr, R C

“…The exoglucanase gene (cex) and the endoglucanase A gene (cenA) from Cellulomonas fimi were subcloned into the Escherichia coli/Brevibacterium lactofermentum…”
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A bifunctional exoglucanase-endoglucanase fusion protein by Warren, R A, Gerhard, B, Gilkes, N R, Owolabi, J B, Kilburn, D G, Miller, Jr, R C

“…A fusion was constructed between the cex gene of Cellulomonas fimi, which encodes an exoglucanase, and the cenA gene of the same organism, which encodes an…”
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Exploring the Cellulose/Xylan Specificity of the β-1,4-Glycanase Cex from Cellulomonas fimi through Crystallography and Mutation by Notenboom, Valerie, Birsan, Camelia, Warren, R. Antony J, Withers, Stephen G, Rose, David R

“…The retaining β-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain…”
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The Acid/Base Catalyst in the Exoglucanase/Xylanase from Cellulomonas fimi Is Glutamic Acid 127: Evidence from Detailed Kinetic Studies of Mutants by MacLeod, Alasdair M, Lindhorst, Thisbe, Withers, Stephen G, Warren, R. Antony J

“…The exoglucanase/xylanase Cex from Cellulomonas fimi hydrolyzes beta-1,4-glycosidic bonds with net retention of anomeric configuration, releasing the…”
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Mannanase Man26A from Cellulomonas fimi has a mannan-binding module by Stoll, D, Boraston, A, Stalbrand, H, McLean, B.W, Kilburn, D.G, Warren, R.A.J

“…A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble…”
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Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein by Doheny, J.G, Jervis, E.J, Guarna, M.M, Humphries, R.K, Warren, R.A.J, Kilburn, D.G

“…A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The…”
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The identification of the catalytic nucleophiles of two β-galactosidases from glycoside hydrolase family 35 by Blanchard, Jan E, Gal, Laurent, He, Shouming, Foisy, Janine, Warren, R.Antony J, Withers, Stephen G

“…The β-galactosidases from Xanthomonas manihotis (β-Gal Xmn) and Bacillus circulans (β-Gal-3 Bcir) are retaining glycosidases that hydrolyze glycosidic bonds…”
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Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi by Shen, H, Gilkes, N.R, Kilburn, D.G, Miller, R.C. Jr, Warren, R.A.J

“…The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long,…”
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Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties by Kwan, Emily, Guarna, M. Marta, Boraston, Alisdair B., Gilkes, Neil R., Haynes, Charles A., Kilburn, Douglas G., Warren, R. Antony J.

“…In this work, a new derivative of FX was engineered. It comprises a cellulose‐binding module (CBM) fused to the N‐terminus of the truncated light chain (E2FX)…”
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Analysis of Molecular Size Distributions of Cellulose Molecules during Hydrolysis of Cellulose by Recombinant Cellulomonas fimiβ-1,4-Glucanases by STALBRAND, H, MANSFIELD, S. D, SADDLER, J. N, KILBURN, D. G, WARREN, R. A. J, GILKES, N. R

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Analysis of molecular size distributions of cellulose molecule by recombinant Cellulomonas fimi beta-1,4-glucanases by Stalbrand, H, Mansfield, S.D, Saddler, J.N, Kilburn, D.G, Warren, R.A.J, Gilkes, N.R

“…Four beta-1,4-glucanases (cellulases) of the cellulolytic bacterium Cellulomonas fimi were purified from Escherichia coli cells transformed with recombinant…”
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Degradation of cellulases in cultures of Cellulomonas fimi by Sandercock, L.E., Meinke, A., Gilkes, N.R., Kilburn, D.G., Warren, R.A.J.

“…Endoglucanases CenA, CenB and CenD, cellobiohydrolases CbhA and CbhB, and the mixed function xylanase-exoglucanase Cex are degraded proteolytically in the…”
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Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue by Lawson, S.L, Warren, R.A.J, Withers, S.G

“…Retaining glycosidases achieve the hydrolysis of glycosidic bonds through the assistance of two key active-site carboxyls. One carboxyl functions as a…”
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