Search Results - "Warren, RAJ"

Thioglycoligases: Mutant Glycosidases for Thioglycoside Synthesis by Jahn, Michael, Marles, Jennifer, Warren, R. Antony J., Withers, Stephen G.

“…Engineering of the active site of retaining glycosidases in conjunction with the appropriate modification of their substrates has led to the enzymatic…”
Get full text

Glycosynthases:  Mutant Glycosidases for Oligosaccharide Synthesis by Mackenzie, Lloyd F, Wang, Qingping, Warren, R. Antony J, Withers, Stephen G

Get full text

Directed Evolution of a Glycosynthase from Agrobacterium sp. Increases Its Catalytic Activity Dramatically and Expands Its Substrate Repertoire by Kim, Young-Wan, Lee, Seung Seo, Warren, R Antony J, Withers, Stephen G

“…The Agrobacterium sp. β-glucosidase (Abg) is a retaining β-glycosidase and its nucleophile mutants, termed Abg glycosynthases, catalyze the formation of…”
Get full text

A scheme for designating enzymes that hydrolyse the polysaccharides in the cell walls of plants by Henrissat, B, Teeri, T.T, Warren, R.A.J

“…A scheme is proposed for designating enzymes that hydrolyse the polysaccharides in the cell walls of plants. These enzymes are predominantly β-1,4-glycanases…”
Get full text

Characterization of a β‐N‐acetylhexosaminidase and a β‐N‐acetylglucosaminidase/β‐glucosidase from Cellulomonas fimi by Mayer, Christoph, Vocadlo, David J., Mah, Melanie, Rupitz, Karen, Stoll, Dominik, Warren, R. A. J., Withers, Stephen G.

“…The Gram‐positive soil bacterium Cellulomonas fimi is shown to produce at least two intracellular β‐N‐acetylglucosaminidases, a family 20…”
Get full text

MICROBIAL HYDROLYSIS OF POLYSACCHARIDES by Warren, R. A. J

“…Microorganisms are efficient degraders of starch, chitin, and the polysaccharides in plant cell walls. Attempts to purify hydrolases led to the realization…”
Get full text

Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis by Jahn, Michael, Chen, Hongming, Müllegger, Johannes, Marles, Jennifer, Warren, R Antony J, Withers, Stephen G

“…A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside…”
Get more information

Recognition and Hydrolysis of Noncrystalline Cellulose by Boraston, Alisdair B, Kwan, Emily, Chiu, Patrick, Warren, R Antony J, Kilburn, Douglas G

“…Cellulase Cel5A from alkalophilic Bacillus sp. 1139 contains a family 17 carbohydrate-binding module ( Bsp CBM17) and a family 28 CBM ( Bsp CBM28) in tandem…”
Get full text

Glycosylation of a Neoglycoprotein by Using Glycosynthase and Thioglycoligase Approaches: The Generation of a Thioglycoprotein by Müllegger, Johannes, Chen, Hong Ming, Warren, R. Antony J., Withers, Stephen G.

“…Remodeling: Glycosylation of a neoglycoprotein was achieved in quantitative yields by using a glycosynthase or a thioglycoligase. The resulting glycoproteins…”
Get full text

Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi by Kim, Young-Wan, Fox, David T, Hekmat, Omid, Kantner, Terrence, McIntosh, Lawrence P, Warren, R Antony J, Withers, Stephen G

“…Glycosynthases are synthetic enzymes derived from retaining glycosidases in which the catalytic nucleophile has been replaced. The mutation allows irreversible…”
Get more information

Structure and Ligand Binding of Carbohydrate-binding Module CsCBM6-3 Reveals Similarities with Fucose-specific Lectins and “Galactose-binding” Domains by Boraston, Alisdair B, Notenboom, Valerie, Warren, R.Antony J, Kilburn, Douglas G, Rose, David R, Davies, Gideon

“…Carbohydrate-binding polypeptides, including carbohydrate-binding modules (CBMs) from polysaccharidases, and lectins, are widespread in nature. Whilst CBMs are…”
Get full text

The E358S mutant of Agrobacterium sp. β-glucosidase is a greatly improved glycosynthase by Mayer, Christoph, Zechel, David L., Reid, Stephen P., Warren, R.Antony J., Withers, Stephen G.

“…Glycosynthases are nucleophile mutants of retaining glycosidases that catalyze the glycosylation of sugar acceptors using glycosyl fluoride donors, thereby…”
Get full text

Carbohydrate-binding Modules Recognize Fine Substructures of Cellulose by McLean, Bradley W., Boraston, Alisdair B., Brouwer, Darren, Sanaie, Nooshafarin, Fyfe, Colin A., Warren, R. Antony J., Kilburn, Douglas G., Haynes, Charles A.

“…Competition isotherms are used to identify the set of cellulose substructures to which cellulose binding modules (CBMs) from families 2a, 3, 4, 9, and 17 bind…”
Get full text

Binding Specificity and Thermodynamics of a Family 9 Carbohydrate-Binding Module from Thermotoga maritima Xylanase 10A by Boraston, Alisdair B, Creagh, A. Louise, Alam, Md. Mustafa, Kormos, Jeffery M, Tomme, Peter, Haynes, Charles A, Warren, R. Antony J, Kilburn, Douglas G

“…The C-terminal family 9 carbohydrate-binding module of xylanase 10A from Thermotoga maritima (CBM9-2) binds to amorphous cellulose, crystalline cellulose, and…”
Get full text

Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides by Jahn, Michael, Stoll, Dominik, Warren, R Antony J, Szabó, Lóránd, Singh, Pritpal, Gilbert, Harry J, Ducros, Valérie M A, Davies, Gideon J, Withers, Stephen G

“…Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined…”
Get more information

Solid-Phase Oligosaccharide and Glycopeptide Synthesis Using Glycosynthases by Tolborg, Jakob F, Petersen, Lars, Jensen, Knud J, Mayer, Christoph, Jakeman, David L, Warren, R. Antony J, Withers, Stephen G

“…Enzymatic approaches for the preparation of oligosaccharides are interesting alternatives to traditional chemical synthesis, the main advantage being the…”
Get full text

Engineering of a thioglycoligase: randomized mutagenesis of the acid–base residue leads to the identification of improved catalysts by Müllegger, Johannes, Jahn, Michael, Chen, Hong-Ming, Warren, R.Antony J., Withers, Stephen G.

“…Thioglycoligases are recently introduced variants of retaining glycosidases in which the acid–base catalyst has been mutated, rendering them capable of…”
Get full text

Mechanism, Mutagenesis, and Chemical Rescue of a β-Mannosidase from Cellulomonas fimi by Zechel, David L., Reid, Stephen P., Stoll, Dominik, Nashiru, Oyekanmi, Warren, R. Antony J., Withers, Stephen G.

“…The chemical mechanism of a retaining β-mannosidase from Cellulomonas fimi has been characterized through steady-state kinetic analyses with a range of…”
Get full text

Domains in microbial β-1,4-glycanases : sequence conservation, function, and enzyme families by GILKES, N. R, HENRISSAT, B, KILBURN, D. G, MILLER, R. C, WARREN, R. A. J

“…Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains…”
Get full text

Mannan-degrading enzymes from Cellulomonas fimi by STOLL, D, STALBRAND, H, WARREN, R. A. J

“…The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and beta-mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted,…”
Get full text