Search Results - "Warren, R. Antony J."

Glycosynthase-Mediated Synthesis of Glycosphingolipids by Vaughan, Mark D, Johnson, Karl, DeFrees, Shawn, Tang, Xiaoping, Warren, R. Antony J, Withers, Stephen G

“…Glycosphingolipids play crucial roles in virtually every stage of the cell cycle, and their clinical administration has been proposed as a treatment for…”
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Structural and Mechanistic Analyses of endo-Glycoceramidase II, a Membrane-associated Family 5 Glycosidase in the Apo and GM3 Ganglioside-bound Forms by Caines, Matthew E.C., Vaughan, Mark D., Tarling, Chris A., Hancock, Susan M., Warren, R. Antony J., Withers, Stephen G., Strynadka, Natalie C.J.

“…endo-Glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of…”
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Solid-Phase Oligosaccharide and Glycopeptide Synthesis Using Glycosynthases by Tolborg, Jakob F, Petersen, Lars, Jensen, Knud J, Mayer, Christoph, Jakeman, David L, Warren, R. Antony J, Withers, Stephen G

“…Enzymatic approaches for the preparation of oligosaccharides are interesting alternatives to traditional chemical synthesis, the main advantage being the…”
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Directed Evolution of a Glycosynthase from Agrobacterium sp. Increases Its Catalytic Activity Dramatically and Expands Its Substrate Repertoire by Kim, Young-Wan, Lee, Seung Seo, Warren, R Antony J, Withers, Stephen G

“…The Agrobacterium sp. β-glucosidase (Abg) is a retaining β-glycosidase and its nucleophile mutants, termed Abg glycosynthases, catalyze the formation of…”
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A Mechanism-Based ICAT Strategy for Comparing Relative Expression and Activity Levels of Glycosidases in Biological Systems by Hekmat, Omid, He, Shouming, Warren, R. Antony J, Withers, Stephen G

“…An activity-based isotope-coded affinity tagging (AB-ICAT) strategy for proteome-wide quantitation of active retaining endoglycosidases has been developed. Two…”
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Recognition and Hydrolysis of Noncrystalline Cellulose by Boraston, Alisdair B, Kwan, Emily, Chiu, Patrick, Warren, R Antony J, Kilburn, Douglas G

“…Cellulase Cel5A from alkalophilic Bacillus sp. 1139 contains a family 17 carbohydrate-binding module ( Bsp CBM17) and a family 28 CBM ( Bsp CBM28) in tandem…”
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Glycosylation of a Neoglycoprotein by Using Glycosynthase and Thioglycoligase Approaches: The Generation of a Thioglycoprotein by Müllegger, Johannes, Chen, Hong Ming, Warren, R. Antony J., Withers, Stephen G.

“…Remodeling: Glycosylation of a neoglycoprotein was achieved in quantitative yields by using a glycosynthase or a thioglycoligase. The resulting glycoproteins…”
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Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi by Kim, Young-Wan, Fox, David T, Hekmat, Omid, Kantner, Terrence, McIntosh, Lawrence P, Warren, R Antony J, Withers, Stephen G

“…Glycosynthases are synthetic enzymes derived from retaining glycosidases in which the catalytic nucleophile has been replaced. The mutation allows irreversible…”
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β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase by Nashiru, Oyekanmi, Zechel, David L., Stoll, Dominik, Mohammadzadeh, Taraneh, Warren, R. Antony J., Withers, Stephen G.

“…Engineering enzymes: The glutamic acid nucleophile of a retaining β‐mannosidase has been replaced with a serine residue to form a β‐mannosynthase. When the new…”
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Thermostable Glycosynthases and Thioglycoligases Derived from Thermotoga maritima β-Glucuronidase by Müllegger, Johannes, Chen, Hong-Ming, Chan, Wing Yiu, Reid, Stephen P, Jahn, Michael, Warren, R. Antony J, Salleh, Hamzah Mohammed, Withers, Stephen G

“…By using active‐site mutants of the Thermotoga maritima β‐glucuronidase, glucuronic acid‐containing glycosides and thioglycosides have been made in high…”
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Carbohydrate-binding Modules Recognize Fine Substructures of Cellulose by McLean, Bradley W., Boraston, Alisdair B., Brouwer, Darren, Sanaie, Nooshafarin, Fyfe, Colin A., Warren, R. Antony J., Kilburn, Douglas G., Haynes, Charles A.

“…Competition isotherms are used to identify the set of cellulose substructures to which cellulose binding modules (CBMs) from families 2a, 3, 4, 9, and 17 bind…”
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Binding Specificity and Thermodynamics of a Family 9 Carbohydrate-Binding Module from Thermotoga maritima Xylanase 10A by Boraston, Alisdair B, Creagh, A. Louise, Alam, Md. Mustafa, Kormos, Jeffery M, Tomme, Peter, Haynes, Charles A, Warren, R. Antony J, Kilburn, Douglas G

“…The C-terminal family 9 carbohydrate-binding module of xylanase 10A from Thermotoga maritima (CBM9-2) binds to amorphous cellulose, crystalline cellulose, and…”
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Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides by Jahn, Michael, Stoll, Dominik, Warren, R Antony J, Szabó, Lóránd, Singh, Pritpal, Gilbert, Harry J, Ducros, Valérie M A, Davies, Gideon J, Withers, Stephen G

“…Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined…”
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Specificity Fingerprinting of Retaining β-1,4-Glycanases in the Cellulomonas fimi Secretome Using Two Fluorescent Mechanism-Based Probes by Hekmat, Omid, Florizone, Christine, Kim, Young-Wan, Eltis, Lindsay D, Warren, R. Antony J, Withers, Stephen G

“…Functional proteomics methods are crucial for activity- and mechanism-based investigation of enzymes in biological systems at a post-translational stage…”
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Co‐operative binding of triplicate carbohydrate‐binding modules from a thermophilic xylanase by Boraston, Alisdair B., McLean, Bradley W., Chen, Grace, Li, Anson, Warren, R. Antony J., Kilburn, Douglas G.

“…Summary Family 6 carbohydrate‐binding modules were am‐plified by polymerase chain reaction (PCR) from Clostridium stercorarium strain NCIB11754 genomic DNA as…”
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Identification and glucan-binding properties of a new carbohydrate-binding module family by Boraston, Alisdair B, Ghaffari, Mazyar, Warren, R Antony J, Kilburn, Douglas G

“…The C-terminal 191-residue module of Cel5A from the alkalophilic Bacillus sp. 1139 comprises a carbohydrate-binding module (CBM) belonging to a previously…”
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N-Glycosidase–carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation by Kwan, Emily M., Boraston, Alisdair B., McLean, Bradley W., Kilburn, Douglas G., Warren, R. Antony J.

“…A carbohydrate-binding module (CBM) was fused to the N-termini of mannosyl-glycoprotein endo-β-N-acetylglucosaminidase (EndoF1) and peptide N-glycosidase F…”
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Specificity and Affinity of Substrate Binding by a Family 17 Carbohydrate-Binding Module from Clostridium cellulovorans Cellulase 5A by Boraston, Alisdair B, Chiu, Patrick, Warren, R. Antony J, Kilburn, Douglas G

“…The C-terminal carbohydrate-binding module (CBM17) from Clostridium cellulovorans cellulase 5A is a β-1,4-glucan binding module with a preference for soluble…”
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The Acid/Base Catalyst in the Exoglucanase/Xylanase from Cellulomonas fimi Is Glutamic Acid 127: Evidence from Detailed Kinetic Studies of Mutants by MacLeod, Alasdair M, Lindhorst, Thisbe, Withers, Stephen G, Warren, R. Antony J

“…The exoglucanase/xylanase Cex from Cellulomonas fimi hydrolyzes beta-1,4-glycosidic bonds with net retention of anomeric configuration, releasing the…”
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Thioglycoligases: Mutant Glycosidases for Thioglycoside Synthesis by Jahn, Michael, Marles, Jennifer, Warren, R. Antony J., Withers, Stephen G.

“…Engineering of the active site of retaining glycosidases in conjunction with the appropriate modification of their substrates has led to the enzymatic…”
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