Comparison between manual and automated total nucleated cell counts using the ADVIA 120 for pleural and peritoneal fluid samples from dogs, cats, horses, and alpacas

Background: Analysis of body fluids includes an estimate of total nucleated cell count (TNCC). Automated methods may enhance the accuracy and timeliness of TNCC results. Objective: The purpose of this report was to assess the ability of the ADVIA 120 hematology analyzer to accurately count nucleated...

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Bibliographic Details
Published in:	Veterinary clinical pathology Vol. 38; no. 3; pp. 388 - 391
Main Authors:	Gorman, M. Elena, Villarroel, Aurora, Tornquist, Susan J, Flachsbart, Joy, Warden, Annette, Boeder, Lisa
Format:	Journal Article
Language:	English
Published:	Malden, USA Blackwell Publishing Inc 01-09-2009
Subjects:	ADVIA 120 alpacas Animals Ascitic Fluid - cytology Automation body fluids Camelids, New World Cats cell count Cell Count - instrumentation Cell Count - methods Cell Count - veterinary Dogs effusion Flow Cytometry - veterinary fluid analysis hematology hemocytometer Horses peritoneal fluid Pleural Effusion - cytology pleural fluids reference standards Reproducibility of Results Sensitivity and Specificity total nucleated cell count veterinary equipment
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Summary:	Background: Analysis of body fluids includes an estimate of total nucleated cell count (TNCC). Automated methods may enhance the accuracy and timeliness of TNCC results. Objective: The purpose of this report was to assess the ability of the ADVIA 120 hematology analyzer to accurately count nucleated cells in pleural and peritoneal fluids from animals, compared with manual counts. Methods: Pleural and peritoneal fluids submitted in EDTA tubes to our laboratory over a 17‐month period were used in the study. TNCC/μL was determined by a manual method, using a hemocytometer, and by an automated method, using the ADVIA 120. Correlation of results was determined by Passing‐Bablok regression, Bland–Altman plots, and Pearson correlation analysis. Results: Samples from dogs (n=36), cats (n=36), horses (n=59), and alpacas (n=11) were analyzed. High correlation in TNCC between methods was found for peritoneal fluid (n=93, r=.959), pleural fluid (n=49, r=.966), and all fluids combined (n=142, r=.960) (P<.001). Variation between methods was greater in samples with TNCCs<1000/μL (r=.62, P<.001). The ADVIA systematically overestimated the number of cells in all fluid samples by 95 cells/μL (confidence interval=19.2–190.5/μL). Conclusion: The ADVIA 120 reliably determines TNCC in pleural and peritoneal effusions and can be recommended for routine veterinary laboratory analysis.
Bibliography:	ark:/67375/WNG-DMGRD38W-S istex:F39B9215453647E5C1CCB0A235483DDB9D5721DB ArticleID:VCP128 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0275-6382 1939-165X
DOI:	10.1111/j.1939-165X.2009.00128.x