Acute and Subchronic Oral Toxicity Evaluation of Herbal Formulation: Piper crocatum Ruiz and Pav., Typhonium flagelliforme (Lodd.) Blume, and Phyllanthus niruri L. in Sprague–Dawley Rats

Acute and Subchronic Oral Toxicity Evaluation of Herbal Formulation: Piper crocatum Ruiz and Pav., Typhonium flagelliforme (Lodd.) Blume, and Phyllanthus niruri L. in Sprague–Dawley Rats

Background. The product combination of Piper crocatum Ruiz. and Pav., Phyllanthus niruri Linn., and Typhonium flagelliforme (Lodd.) BL ethanolic extract (SKM) exerts immunomodulatory activity. However, the toxicity profile of the combination has never been investigated. Objective. This study aimed t...

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Published in:Journal of toxicology Vol. 2023; pp. 7511397 - 11
Main Authors: Murwanti, Retno, Nurrochmad, A., Gani, Andayana P., Sasmito, Ediati, Edwina, Angela E., Chandra, Mayang K., Suryawan, F. H., Wardana, A. R., Natalia, Budiningsih, Jelita L. S. R.
Format: Journal Article
Language:English
Published: Egypt Hindawi 03-01-2023
John Wiley & Sons, Inc
Hindawi Limited
Subjects:
Acute toxicity
Animals
Blood
Body weight
Ethylenediaminetetraacetic acid
Females
Food consumption
Hematology
Hemoglobin
Humidity
Immunomodulation
Kidneys
Laboratories
Liver
Oral administration
Parameters
Phyllanthus niruri
Toxicity
Toxicity testing
Urinalysis
Water consumption
Indonesia
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Summary:Background. The product combination of Piper crocatum Ruiz. and Pav., Phyllanthus niruri Linn., and Typhonium flagelliforme (Lodd.) BL ethanolic extract (SKM) exerts immunomodulatory activity. However, the toxicity profile of the combination has never been investigated. Objective. This study aimed to establish the acute toxicity profile of the SKM product on Sprague–Dawley (SD) rats and its subchronic toxicity profile on female SD rats. Method. The acute and subchronic toxicity tests were conducted in accordance with OECD 423 and OECD 408, respectively. Result. The SKM product was safe up to 5000 mg/kg b.w. in male and female SD rats. In repeated doses of SKM for 90 days, the administration of 22.5, 45, and 90 mg/kg b.w. per day of the SKM product to female SD rats did not affect clinical signs, body weight, food and water consumption, hematological parameters, clinical chemical parameters, urinalysis, relative organ weights, and gross pathological and histopathological features compared with the control group. Conclusion. Analyses of these results suggest that the long-term oral administration of the SKM product for 90 days does not cause subchronic toxicity.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Academic Editor: Mohd. Salman
ISSN:1687-8191
1687-8205
DOI:10.1155/2023/7511397