Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

Water Uptake of Airborne Cells of P. syringae Measured with a Hygroscopicity Tandem Differential Mobility Analyzer

Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i....

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Published in:Environmental science & technology Vol. 58; no. 43; pp. 19211 - 19221
Main Authors: Nielsen, Lærke Sloth, Šantl-Temkiv, Tina, Palomeque Sánchez, María, Massling, Andreas, Ward, Josephine Caroline, Jensen, Pia Bomholt, Boesen, Thomas, Petters, Markus, Finster, Kai, Bilde, Merete, Rosati, Bernadette
Format: Journal Article
Language:English
Published: United States American Chemical Society 29-10-2024
Subjects:
Airborne microorganisms
Analog computers
Cell survival
Dehydration
Efflorescence
Energy and Climate
Growth factors
Hygroscopicity
Ice nucleation
Impact analysis
Microorganisms
Nucleation
Relative humidity
Sodium chloride
Water availability
Water uptake
Water vapor
ice nucleation-active bacteria
HTDMA
hygroscopic growth
clouds
bioaerosols
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Summary:Airborne microorganisms impact cloud formation and are involved in disease spreading. The ability of airborne cells to survive and express genes may be limited by reduced water availability in the atmosphere and depend on the ability of the cells to attract water vapor at subsaturated conditions, i.e., their hygroscopicity. We assessed hygroscopic properties of the plant pathogen Pseudomonas syringae, known to participate in cloud formation. We used a hygroscopicity tandem differential mobility analyzer to examine both hydration and dehydration behavior in the relative humidity (RH) range 5–90%. The cells were aerosolized either from Milli-Q water or from a 35 g L–1 NaCl solution, resulting in pure cells or cells associated with NaCl. Pure cells exhibited no deliquescence/efflorescence and a small gradual water uptake reaching a maximum growth factor (GF) of 1.09 ± 0.01 at 90% RH. For cells associated with NaCl, we observed deliquescence and a much larger maximum GF of 1.74 ± 0.03 at 90% RH. Deliquescence RH was comparable to that of pure NaCl, highlighting the major role of the salt associated with the cells. It remains to be investigated how the observed hygroscopic properties relate to survival, metabolic, and ice-nucleation activities of airborne P. syringae.
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ISSN:0013-936X
1520-5851
1520-5851
DOI:10.1021/acs.est.4c01817