Multiple Strain Colonization and Metronidazole Resistance in Helicobacter pylori-Infected Patients: Identification from Sequential and Multiple Biopsy Specimens

Helicobacter pylori strain diversity was investigated in infected persons by collection of multiple biopsies before and after therapy failure. It was demonstrated by random amplification of polymorphic DNA polymerase chain reaction that patients may be infected with a mixed population of H. pylori s...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 174; no. 3; pp. 631 - 635
Main Authors:	Jorgensen, Margaret, Daskalopoulos, George, Warburton, Verlaine, Mitchell, Hazel M., Hazell, Stuart L.
Format:	Journal Article
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-09-1996 University of Chicago Press Oxford University Press
Subjects:	Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Biopsies Biopsy Bismuth Concise Communications DNA DNA, Bacterial - analysis Drug Resistance, Microbial - genetics Genetic polymorphism Genetic Variation Genomics Helicobacter Infections - drug therapy Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - growth & development Human bacterial diseases Humans Infections Infectious diseases Medical sciences Metronidazole - therapeutic use Microbial colonization Polymerase Chain Reaction Stomach Stomach - microbiology Human Imidazole derivatives Spirillales Spirillaceae Biodiversity Infection Campylobacter infection Microbiological investigation Helicobacter pylori Biopsy Multiple resistance Pathogen strain Bacteriosis Bacteria
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Summary:	Helicobacter pylori strain diversity was investigated in infected persons by collection of multiple biopsies before and after therapy failure. It was demonstrated by random amplification of polymorphic DNA polymerase chain reaction that patients may be infected with a mixed population of H. pylori strains. Most patients were colonized with a predominant strain accompanied by up to 5 variant strains. The use of antimicrobials resulted in an altered distribution of the strains present, but the predominant strain usually remained. Patients may be infected with a mixed population of metronidazole-sensitive and -resistant strains at one time, with metronidazole-based therapy selectively enriching for a resistant population.
Bibliography:	Reprints or correspondence: Dr. Stuart L. Hazell, School of Microbiology and Immunology, University of New South Wales, Sydney, Australia, 2052. istex:7D797F1DDF91C64E5AF832C8F43C1A1C6D3C82D7 ark:/67375/HXZ-W57W12CL-L ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1899 1537-6613
DOI:	10.1093/infdis/174.3.631