Molecular Characterization of Sudanese and Southern Sudanese Chicken Breeds Using mtDNA D-Loop

Molecular Characterization of Sudanese and Southern Sudanese Chicken Breeds Using mtDNA D-Loop

The objective of this study was to assess the genetic relationships and diversity and to estimate the amount of gene flow among the five chicken populations from Sudan and South Sudan and commercial strain of egg line White Leghorn chickens. The chicken populations were genotyped using mtDNA D-loop...

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Bibliographic Details
Published in:Genetics Research International Vol. 2014; no. 2014; pp. k1 - 8
Main Authors: Wani, Charles E., Yousif, Ibrahim A., Ibrahim, Muntasir E., Musa, Hassan H.
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Limiteds 01-01-2014
Hindawi Puplishing Corporation
Hindawi Publishing Corporation
John Wiley & Sons, Inc
Hindawi Limited
Subjects:
Cattle
Chickens
Deoxyribonucleic acid
DNA
Gene flow
Genetic aspects
Genetic diversity
Genetic relationship
Genetic variation
Genomes
Haplotypes
Mitochondrial DNA
Phylogenetics
Phylogeny
Physiological aspects
Population
Population genetics
Poultry
Savannahs
Software
Sudan
China
Germany
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Summary:The objective of this study was to assess the genetic relationships and diversity and to estimate the amount of gene flow among the five chicken populations from Sudan and South Sudan and commercial strain of egg line White Leghorn chickens. The chicken populations were genotyped using mtDNA D-loop as a molecular marker. PCR product of the mtDNA D-loop segment was 600 bp and 14 haplotypes were identified. The neighbor-joining phylogenetic tree indicated that the indigenous Sudanese chickens can be grouped into two clades, IV and IIIa only. Median joining networks analysis showed that haplotype LBB49 has the highest frequency. The hierarchal analysis of molecular variance (AMOVA) showed that genetic variation within the population was 88.6% and the differentiation among the population was 11.4%. When the populations was redefined into two geographical zones, rich and poor Savanna, the results were fractioned into three genetic variations: between individuals within population 95.5%, between populations within the group 0.75%, and genetic variation between groups 3.75%. The pair wise Fst showed high genetic difference between Betwil populations and the rest with Fst ranging from 0.1492 to 0.2447. We found that there is large number of gene exchanges within the Sudanese indigenous chicken (Nm=4.622).
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Academic Editor: Arne Ludwig
ISSN:2090-3154
2090-3162
2090-3162
DOI:10.1155/2014/928420