Search Results - "Wang, Jiuyu"

Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference by You, Lilan, Ma, Jun, Wang, Jiuyu, Artamonova, Daria, Wang, Min, Liu, Liang, Xiang, Hua, Severinov, Konstantin, Zhang, Xinzheng, Wang, Yanli

“…Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic…”
Get full text

Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites by Yin, Maolu, Wang, Jiuyu, Wang, Min, Li, Xinmei, Zhang, Mo, Wu, Qiang, Wang, Yanli

“…CTCF, a conserved 3D genome architecture protein, determines proper genome-wide chromatin looping interactions through directional binding to specific sequence…”
Get full text

Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli by Zhao, Hongtu, Sheng, Gang, Wang, Jiuyu, Wang, Min, Bunkoczi, Gabor, Gong, Weimin, Wei, Zhiyi, Wang, Yanli

“…The CRISPR/Cas system is an RNA-guided bacterial protection system against foreign nucleic acids of bacterial and archaeal origin; here a high-resolution…”
Get full text

An anti-CRISPR that represses its own transcription while blocking Cas9-target DNA binding by Deng, Xieshuting, Sun, Wei, Li, Xueyan, Wang, Jiuyu, Cheng, Zhi, Sheng, Gang, Wang, Yanli

“…AcrIIA15 is an anti-CRISPR (Acr) protein that inhibits Staphylococcus aureus Cas9 (SaCas9). Although previous studies suggested it has dual functions, the…”
Get full text

Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2 by Huang, Xue, Sun, Wei, Cheng, Zhi, Chen, Minxuan, Li, Xueyan, Wang, Jiuyu, Sheng, Gang, Gong, Weimin, Wang, Yanli

“…To understand how the RuvC catalytic domain of Class 2 Cas proteins cleaves DNA, it will be necessary to elucidate the structures of RuvC-containing Cas…”
Get full text

Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage by Sheng, Gang, Zhao, Hongtu, Wang, Jiuyu, Yu Rao, Tian, Wenwen, Swarts, Daan C., Oost, John van der, Patel, Dinshaw J., Wang, Yanli

“…We report on crystal structures of ternary Thermus thermophilus Argonaute (Tt Ago) complexes with 5′-phosphorylated guide DNA and a series of DNA targets…”
Get full text

A CRISPR evolutionary arms race： structural insights into viral anti-CRISPR/Cas responses by Wang, Jiuyu, Ma, Jun, Cheng, Zhi, Meng, Xu, You, Lilan, Wang, Min, Zhang, Xinzheng, Wang, Yanli

“…Dear Editor, CRISPR/Cas systems are widespread RNA-mediated prokaryotic adaptive immune systems providing protection against invading nucleic acids . However,…”
Get full text

Crystal structure of Cas1 in complex with branched DNA by Yang, Jing, Li, Jiazhi, Wang, Jiuyu, Sheng, Gang, Wang, Min, Zhao, Hongtu, Yang, Yanhua, Wang, Yanli

“…Cas1 is a key component of the CRISPR adaptation complex, which captures and integrates foreign DNA into the CRISPR array, resulting in the generation of new…”
Get full text

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a by Liu, Liang, Li, Xueyan, Ma, Jun, Li, Zongqiang, You, Lilan, Wang, Jiuyu, Wang, Min, Zhang, Xinzheng, Wang, Yanli

“…Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA…”
Get full text

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems by Wang, Jiuyu, Li, Jiazhi, Zhao, Hongtu, Sheng, Gang, Wang, Min, Yin, Maolu, Wang, Yanli

“…Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR…”
Get full text

Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities by Liu, Liang, Li, Xueyan, Wang, Jiuyu, Wang, Min, Chen, Peng, Yin, Maolu, Li, Jiazhi, Sheng, Gang, Wang, Yanli

“…C2c2, the effector of type VI CRISPR-Cas systems, has two RNase activities—one for cutting its RNA target and the other for processing the CRISPR RNA (crRNA)…”
Get full text

C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism by Liu, Liang, Chen, Peng, Wang, Min, Li, Xueyan, Wang, Jiuyu, Yin, Maolu, Wang, Yanli

“…C2c1 is a type V-B CRISPR-Cas system dual-RNA-guided DNA endonuclease. Here, we report the crystal structure of Alicyclobacillus acidoterrestris C2c1 in…”
Get full text

Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States by Sun, Wei, Yang, Jing, Cheng, Zhi, Amrani, Nadia, Liu, Chao, Wang, Kangkang, Ibraheim, Raed, Edraki, Alireza, Huang, Xue, Wang, Min, Wang, Jiuyu, Liu, Liang, Sheng, Gang, Yang, Yanhua, Lou, Jizhong, Sontheimer, Erik J., Wang, Yanli

“…High-resolution Cas9 structures have yet to reveal catalytic conformations due to HNH nuclease domain positioning away from the cleavage site. Nme1Cas9 and…”
Get full text

Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition by Sun, Wei, Zhao, Xiaolong, Wang, Jinlong, Yang, Xiaoqi, Cheng, Zhi, Liu, Shuo, Wang, Jiuyu, Sheng, Gang, Wang, Yanli

“…Anti-CRISPR proteins are encoded by phages to inhibit the CRISPR-Cas systems of the hosts. AcrIIC5 inhibits several naturally high-fidelity type II-C Cas9…”
Get full text

AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation by Sun, Wei, Cheng, Zhi, Wang, Jiuyu, Yang, Jing, Li, Xueyan, Wang, Jinlong, Chen, Minxuan, Yang, Xiaoqi, Sheng, Gang, Lou, Jizhong, Wang, Yanli

“…Anti-CRISPR (Acr) proteins are encoded by phages and other mobile genetic elements and inhibit host CRISPR-Cas immunity using versatile strategies. AcrIIC4 is…”
Get full text

An anti-CRISPR that pulls apart a CRISPR–Cas complex by Trost, Chantel N., Yang, Jing, Garcia, Bianca, Hidalgo-Reyes, Yurima, Fung, Beatrice C. M., Wang, Jiuyu, Lu, Wang-Ting, Maxwell, Karen L., Wang, Yanli, Davidson, Alan R.

“…In biological systems, the activities of macromolecular complexes must sometimes be turned off. Thus, a wide variety of protein inhibitors has evolved for this…”
Get full text

Control-oriented planning and development-oriented planning: comparison of planning paradigms and practice in Hubei, China by Zheng, Degao, Chen, Ye, Luo, Yan, Wang, Jiuyu

“…Abstract The history of planning development in various countries shows that the core logic of planning is the spiral interweaving of control and development,…”
Get full text

Pathogen protein modularity enables elaborate mimicry of a host phosphatase by Li, Hui, Wang, Jinlong, Kuan, Tung Ariel, Tang, Bozeng, Feng, Li, Wang, Jiuyu, Cheng, Zhi, Skłenar, Jan, Derbyshire, Paul, Hulin, Michelle, Li, Yufei, Zhai, Yi, Hou, Yingnan, Menke, Frank L.H., Wang, Yanli, Ma, Wenbo

“…Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an effector repertoire is…”
Get full text

Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes by Sheng, Gang, Gogakos, Tasos, Wang, Jiuyu, Zhao, Hongtu, Serganov, Artem, Juranek, Stefan, Tuschl, Thomas, Patel, Dinshaw J, Wang, Yanli

“…We have undertaken a systematic structural study of Thermus thermophilus Argonaute (TtAgo) ternary complexes containing single-base bulges positioned either…”
Get full text