Catalase prevents lipid peroxidation and enhances survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing medium

The objectives of this study were to determine: 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium s...

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Bibliographic Details
Published in:	Biopreservation and biobanking Vol. 10; no. 4; p. 338
Main Authors:	Luz, Hiedely K M, Santos, Regiane R, Wanderley, Livia S, Faustino, Luciana R, Silva, Cleidson M G, Carvalho, Adeline A, Campello, Claudio C, Santos, Francielli W, Figueiredo, José R, Rodrigues, Ana Paula R
Format:	Journal Article
Language:	English
Published:	United States 01-08-2012
Subjects:	Animals Catalase - metabolism Cryopreservation - methods Cryoprotective Agents - adverse effects Female Freezing Goats Lipid Peroxidation - drug effects Ovarian Follicle - drug effects Ovarian Follicle - enzymology Ovarian Follicle - metabolism Ovary - drug effects Ovary - enzymology Ovary - metabolism Propylene Glycol - adverse effects
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Summary:	The objectives of this study were to determine: 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium supplementation with Trolox(®) (0.1, 0.5, or 1.0 mM) or catalase (5, 10, or 20 IU/mL) on follicular morphology, viability, and lipid peroxidation. Cryopreservation decreased (p<0.05) percentages of normal follicles relative to the control (84%). Although supplementation of the cryopreservation medium (1.0 M PROH) with catalase (10 or 20 IU/mL) or Trolox(®) (0.1 mM) resulted in follicular morphology and viability similar to that in the controls (P>0.05), lipid peroxidation was reduced only when 20 IU/mL catalase was added to the cryopreservation medium.
ISSN:	1947-5543
DOI:	10.1089/bio.2011.0046