Search Results - "Wan, Yuk Kei"

A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets by Zhou, Peng, Chan, Becky K.C., Wan, Yuk Kei, Yuen, Chaya T.L., Choi, Gigi C.G., Li, Xinran, Tong, Cindy S.W., Zhong, Sophia S.W., Sun, Jieran, Bao, Yufan, Mak, Silvia Y.L., Chow, Maggie Z.Y., Khaw, Jien Vei, Leung, Suet Yi, Zheng, Zongli, Cheung, Lydia W.T., Tan, Kaeling, Wong, Koon Ho, Chan, H.Y. Edwin, Wong, Alan S.L.

“…We present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en…”
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Extensible combinatorial CRISPR screening in mammalian cells by Zhou, Peng, Wan, Yuk Kei, Chan, Becky K.C., Choi, Gigi C.G., Wong, Alan S.L.

“…The CRISPR-Cas system coupled with Combinatorial Genetics En Masse (CombiGEM) enables systematic analysis of high-order genetic perturbations that are…”
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Beyond sequencing: machine learning algorithms extract biology hidden in Nanopore signal data by Wan, Yuk Kei, Hendra, Christopher, Pratanwanich, Ploy N., Göke, Jonathan

“…Nanopore sequencing provides signal data corresponding to the nucleotide motifs sequenced. Through machine learning-based methods, these signals are translated…”
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Detection of m6A from direct RNA sequencing using a multiple instance learning framework by Hendra, Christopher, Pratanwanich, Ploy N., Wan, Yuk Kei, Goh, W. S. Sho, Thiery, Alexandre, Göke, Jonathan

“…RNA modifications such as m6A methylation form an additional layer of complexity in the transcriptome. Nanopore direct RNA sequencing can capture this…”
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Context-aware transcript quantification from long-read RNA-seq data with Bambu by Chen, Ying, Sim, Andre, Wan, Yuk Kei, Yeo, Keith, Lee, Joseph Jing Xian, Ling, Min Hao, Love, Michael I., Göke, Jonathan

“…Most approaches to transcript quantification rely on fixed reference annotations; however, the transcriptome is dynamic and depending on the context, such…”
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Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore by Pratanwanich, Ploy N., Yao, Fei, Chen, Ying, Koh, Casslynn W. Q., Wan, Yuk Kei, Hendra, Christopher, Poon, Polly, Goh, Yeek Teck, Yap, Phoebe M. L., Chooi, Jing Yuan, Chng, Wee Joo, Ng, Sarah B., Thiery, Alexandre, Goh, W. S. Sho, Göke, Jonathan

“…RNA modifications, such as N 6 -methyladenosine (m 6 A), modulate functions of cellular RNA species. However, quantifying differences in RNA modifications has…”
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Systematic assessment of long-read RNA-seq methods for transcript identification and quantification by Pardo-Palacios, Francisco J., Wang, Dingjie, Reese, Fairlie, Diekhans, Mark, Carbonell-Sala, Sílvia, Williams, Brian, Loveland, Jane E., De María, Maite, Adams, Matthew S., Balderrama-Gutierrez, Gabriela, Behera, Amit K., Gonzalez Martinez, Jose M., Hunt, Toby, Lagarde, Julien, Liang, Cindy E., Li, Haoran, Meade, Marcus Jerryd, Moraga Amador, David A., Prjibelski, Andrey D., Birol, Inanc, Bostan, Hamed, Brooks, Ashley M., Çelik, Muhammed Hasan, Chen, Ying, Du, Mei R. M., Felton, Colette, Göke, Jonathan, Hafezqorani, Saber, Herwig, Ralf, Kawaji, Hideya, Lee, Joseph, Li, Jian-Liang, Lienhard, Matthias, Mikheenko, Alla, Mulligan, Dennis, Nip, Ka Ming, Pertea, Mihaela, Ritchie, Matthew E., Sim, Andre D., Tang, Alison D., Wan, Yuk Kei, Wang, Changqing, Wong, Brandon Y., Yang, Chen, Barnes, If, Berry, Andrew E., Capella-Gutierrez, Salvador, Cousineau, Alyssa, Dhillon, Namrita, Fernandez-Gonzalez, Jose M., Ferrández-Peral, Luis, Garcia-Reyero, Natàlia, Götz, Stefan, Hernández-Ferrer, Carles, Kondratova, Liudmyla, Liu, Tianyuan, Martinez-Martin, Alessandra, Menor, Carlos, Mestre-Tomás, Jorge, Mudge, Jonathan M., Panayotova, Nedka G., Paniagua, Alejandro, Repchevsky, Dmitry, Ren, Xingjie, Rouchka, Eric, Saint-John, Brandon, Sapena, Enrique, Sheynkman, Leon, Smith, Melissa Laird, Suner, Marie-Marthe, Takahashi, Hazuki, Youngworth, Ingrid A., Carninci, Piero, Denslow, Nancy D., Guigó, Roderic, Hunter, Margaret E., Maehr, Rene, Shen, Yin, Tilgner, Hagen U., Wold, Barbara J., Vollmers, Christopher, Frankish, Adam, Au, Kin Fai, Sheynkman, Gloria M., Mortazavi, Ali, Conesa, Ana, Brooks, Angela N.

“…The Long-read RNA-Seq Genome Annotation Assessment Project Consortium was formed to evaluate the effectiveness of long-read approaches for transcriptome…”
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High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis by Wan, Yuk Kei, Choi, Gigi C G, Wong, Alan S L

“…Exploring how combinatorial mutations can be combined to optimize protein functions is important to guide protein engineering. Given the vast combinatorial…”
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Extensible benchmarking of methods that identify and quantify polyadenylation sites from RNA-seq data by Bryce-Smith, Sam, Burri, Dominik, Gazzara, Matthew R, Herrmann, Christina J, Danecka, Weronika, Fitzsimmons, Christina M, Wan, Yuk Kei, Zhuang, Farica, Fansler, Mervin M, Fernández, José M, Ferret, Meritxell, Gonzalez-Uriarte, Asier, Haynes, Samuel, Herdman, Chelsea, Kanitz, Alexander, Katsantoni, Maria, Marini, Federico, McDonnel, Euan, Nicolet, Ben, Poon, Chi-Lam, Rot, Gregor, Schärfen, Leonard, Wu, Pin-Jou, Yoon, Yoseop, Barash, Yoseph, Zavolan, Mihaela

“…The tremendous rate with which data is generated and analysis methods emerge makes it increasingly difficult to keep track of their domain of applicability,…”
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