Activation and Transcriptional Interaction between agr RNAII and RNAIII in Staphylococcus aureus In Vitro and in an Experimental Endocarditis Model

This study compared the promoter activation profiles of the 2 major transcripts of the Staphylococcus aureus global regulon, agr (RNAII and RNAIII). In vitro, RNAIII activation temporally followed RNAII activation and was absent in agr mutants. In experimental endocarditis, maximal RNAII activation...

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Bibliographic Details
Published in:	The Journal of infectious diseases Vol. 186; no. 5; pp. 668 - 677
Main Authors:	Xiong, Yan-Qiong, Wamel, William Van, Nast, Cynthia C., Yeaman, Michael R., Cheung, Ambrose L., Bayer, Arnold S.
Format:	Journal Article
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-09-2002 University of Chicago Press Oxford University Press
Subjects:	Animals Bacterial diseases Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Cytometry Disease Models, Animal Endocarditis Endocarditis, Bacterial - blood Endocarditis, Bacterial - microbiology Experimental bacterial diseases and models Female Flow Cytometry Fluorescence Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - genetics Genes Genetics Infections Infectious diseases Kidneys Major Article Medical sciences Microbiology Plasmids Promoter Regions, Genetic Rabbits Regression Analysis RNA, Bacterial - genetics RNA, Bacterial - metabolism Staphylococcal Infections - blood Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - genetics Trans-Activators - genetics Trans-Activators - metabolism Transcriptional Activation Vegetation Virulence Transcription Rabbit Cardiovascular disease Lagomorpha Gene expression Infection Vertebrata Experimental disease Mammalia Heart disease Endocardial disease Endocarditis Bacteriosis Bacteria Micrococcales Micrococcaceae Staphylococcal infection Staphylococcus aureus
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Summary:	This study compared the promoter activation profiles of the 2 major transcripts of the Staphylococcus aureus global regulon, agr (RNAII and RNAIII). In vitro, RNAIII activation temporally followed RNAII activation and was absent in agr mutants. In experimental endocarditis, maximal RNAII activation in vegetations occurred early, followed by progressive increases in RNAIII activation (P<.05; 2 vs. 48 h); this paralleled significant increases in vegetation bacterial densities over time (P<.05; 2 and 6 vs. 48 h). At 48 h of infection, S. aureus densities in vegetations were significantly higher than those in kidney or spleen tissue (P<.05), paralleling a significantly greater RNAIII activation profile in vegetations than in the latter tissues (P<.05). Of importance, RNAIII activation was observed in vegetations in 2 agr mutants. These data demonstrate that RNAIII activation in vivo is time and cell density dependent, may be tissue specific, and can occur through RNAII-dependent and -independent mechanisms
Bibliography:	istex:876E15DF68A5D2E69011068C22B360A76D649EAF ark:/67375/HXZ-V2VN2FPX-C ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1899 1537-6613
DOI:	10.1086/342046