Search Results - "Walt, David R"

Highly Sensitive and Multiplexed Protein Measurements by Cohen, Limor, Walt, David R

“…Proteins are involved in many biological processes. Misfolded, truncated, or mutated proteins as well as over- or underexpressed proteins have been implicated…”
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Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays by Wu, Connie, Garden, Padric M, Walt, David R

“…Measurements of very low levels of biomolecules, including proteins and nucleic acids, remain a critical challenge in many clinical diagnostic applications due…”
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Single‐Molecule Analysis Determines Isozymes of Human Alkaline Phosphatase in Serum by Jiang, Yu, Li, Xiang, Walt, David R.

“…Alkaline phosphatase (ALP) is an important biomarker, as high levels of ALP in blood can indicate liver disease or bone disorders. However, current clinical…”
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Single-Molecule Arrays for Protein and Nucleic Acid Analysis by Cohen, Limor, Walt, David R

“…The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement…”
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Digital direct detection of microRNAs using single molecule arrays by Cohen, Limor, Hartman, Mark R, Amardey-Wellington, Aaron, Walt, David R

“…MicroRNAs (miRNAs) are involved in many biological pathways, and detecting miRNAs accurately is critical for diagnosing a variety of diseases including cancer…”
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Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering by Kelley, Shana O., Mirkin, Chad A., Walt, David R., Ismagilov, Rustem F., Toner, Mehmet, Sargent, Edward H.

“…This article reviews the different strategies and devices that combine nanoscale and microscale materials to bring about faster, more sensitive and reliable…”
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Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays by Wang, Xu, Ogata, Alana F, Walt, David R

“…Enzyme assays are important for many applications including clinical diagnostics, functional proteomics, and drug discovery. Current methods for enzymatic…”
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Protein measurements in microwells by Walt, David R

“…Protein measurements are essential to many fields ranging from fundamental biochemistry to clinical diagnostics. The ability to make measurements of proteins…”
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Donor Clonal Hematopoiesis and Recipient Outcomes After Transplantation by Gibson, Christopher J, Kim, Haesook T, Zhao, Lin, Murdock, H Moses, Hambley, Bryan, Ogata, Alana, Madero-Marroquin, Rafael, Wang, Shiyu, Green, Lisa, Fleharty, Mark, Dougan, Tyler, Cheng, Chi-An, Blumenstiel, Brendan, Cibulskis, Carrie, Tsuji, Junko, Duran, Madeleine, Gocke, Christopher D, Antin, Joseph H, Nikiforow, Sarah, DeZern, Amy E, Chen, Yi-Bin, Ho, Vincent T, Jones, Richard J, Lennon, Niall J, Walt, David R, Ritz, Jerome, Soiffer, Robert J, Gondek, Lukasz P, Lindsley, R Coleman

“…Clonal hematopoiesis (CH) can be transmitted from a donor to a recipient during allogeneic hematopoietic cell transplantation. Exclusion of candidate donors…”
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Framework for rapid comparison of extracellular vesicle isolation methods by Ter-Ovanesyan, Dmitry, Norman, Maia, Lazarovits, Roey, Trieu, Wendy, Lee, Ju-Hyun, Church, George M, Walt, David R

“…Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as reservoirs of disease biomarkers. One of the main challenges in…”
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Plasma IL-6 changes correlate to PD-1 inhibitor responses in NSCLC by Keegan, Alissa, Ricciuti, Biagio, Garden, Padric, Cohen, Limor, Nishihara, Reiko, Adeni, Anika, Paweletz, Cloud, Supplee, Julianna, Jänne, Pasi A, Severgnini, Mariano, Awad, Mark M, Walt, David R

“…BackgroundBlood-based biomarkers of anti-solid tumor immune checkpoint blockade (ICB) response are lacking. We hypothesized that changes in systemic cytokine…”
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Mechanistic Aspects of Horseradish Peroxidase Elucidated through Single-Molecule Studies by Gorris, Hans H, Walt, David R

“…Many individual horseradish peroxidase (HRP) molecules were isolated and observed simultaneously by fluorescence microscopy in an array of 50 000 femtoliter…”
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Salivary Diagnostics Using a Portable Point-of-Service Platform: A Review by Khanna, Prarthana, PhD Candidate, Walt, David R., PhD

“…Abstract Clinical diagnostics can be improved by faster and more accessible disease detection. Our laboratory has developed a point-of-service (POS) device…”
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Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level by Deiss, Frédérique, LaFratta, Christopher N, Symer, Matthew, Blicharz, Timothy M, Sojic, Neso, Walt, David R

“…A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is…”
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Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine by Barney, Bryan T, Munkholm, Christiane, Walt, David R, Palumbi, Stephen R

“…Atlantic cod (Gadus morhua), is known to vary genetically across the North Atlantic, Greenland, and Newfoundland. This genetic variation occurs both spatially…”
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Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins by Ter-Ovanesyan, Dmitry, Gilboa, Tal, Budnik, Bogdan, Nikitina, Adele, Whiteman, Sara, Lazarovits, Roey, Trieu, Wendy, Kalish, David, Church, George M, Walt, David R

“…Extracellular vesicles (EVs) are released by all cells into biofluids such as plasma. The separation of EVs from highly abundant free proteins and similarly…”
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Analytical Chemistry on the Femtoliter Scale by Gorris, Hans H, Walt, David R

“…The compartmentalization of reactions in femtoliter (fL) containers and integration of fL containers into arrays not only enhances and accelerates chemical and…”
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Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines by Cohen, Limor, Keegan, Alissa, Melanson, Stacy E.F., Walt, David R.

“…In this study, we evaluated the impact of clinical sample handling and processing on IL-6, IL-10, IFNγ, and IL-2 measurements in plasma. We collected whole…”
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Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip by Goyal, Girija, Prabhala, Pranav, Mahajan, Gautam, Bausk, Bruce, Gilboa, Tal, Xie, Liangxia, Zhai, Yunhao, Lazarovits, Roey, Mansour, Adam, Kim, Min Sun, Patil, Aditya, Curran, Danielle, Long, Jaclyn M., Sharma, Sanjay, Junaid, Abidemi, Cohen, Limor, Ferrante, Thomas C., Levy, Oren, Prantil‐Baun, Rachelle, Walt, David R., Ingber, Donald E.

“…Lymphoid follicles (LFs) are responsible for generation of adaptive immune responses in secondary lymphoid organs and form ectopically during chronic…”
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Humoral profiles of toddlers and young children following SARS-CoV-2 mRNA vaccination by Nziza, Nadège, Deng, Yixiang, Wood, Lianna, Dhanoa, Navneet, Dulit-Greenberg, Naomi, Chen, Tina, Kane, Abigail S., Swank, Zoe, Davis, Jameson P., Demokritou, Melina, Chitnis, Anagha P., Fasano, Alessio, Edlow, Andrea G., Jain, Nitya, Horwitz, Bruce H., McNamara, Ryan P., Walt, David R., Lauffenburger, Douglas A., Julg, Boris, Shreffler, Wayne G., Alter, Galit, Yonker, Lael M.

“…Although young children generally experience mild symptoms following infection with SARS-CoV-2, severe acute and long-term complications can occur. SARS-CoV-2…”
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