Search Results - "Walsh, Alex"

POSEA: A novel algorithm to evaluate the performance of multi-object instance image segmentation by Wang, Nianchao, Hu, Linghao, Walsh, Alex J

“…Many techniques and software packages have been developed to segment individual cells within microscopy images, necessitating a robust method to evaluate…”
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Evaluation of Cellpose segmentation with sequential thresholding for instance segmentation of cytoplasms within autofluorescence images by Wang, Nianchao, Hu, Linghao, Walsh, Alex J.

“…Autofluorescence imaging of the coenzyme, reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), provides a label-free technique to assess cellular…”
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Quantitative optical imaging of primary tumor organoid metabolism predicts drug response in breast cancer by Walsh, Alex J, Cook, Rebecca S, Sanders, Melinda E, Aurisicchio, Luigi, Ciliberto, Gennaro, Arteaga, Carlos L, Skala, Melissa C

“…There is a need for technologies to predict the efficacy of cancer treatment in individual patients. Here, we show that optical metabolic imaging of organoids…”
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Optical Metabolic Imaging Identifies Glycolytic Levels, Subtypes, and Early-Treatment Response in Breast Cancer by WALSH, Alex J, COOK, Rebecca S, MANNING, H. Charles, HICKS, Donna J, LAFONTANT, Alec, ARTEAGA, Carlos L, SKALA, Melissa C

“…Abnormal cellular metabolism is a hallmark of cancer, yet there is an absence of quantitative methods to dynamically image this powerful cellular function…”
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Signal Transducer and Activator of Transcription 3, Mediated Remodeling of the Tumor Microenvironment Results in Enhanced Tumor Drug Delivery in a Mouse Model of Pancreatic Cancer by Nagathihalli, Nagaraj S, Castellanos, Jason A, Shi, Chanjuan, Beesetty, Yugandhar, Reyzer, Michelle L, Caprioli, Richard, Chen, Xi, Walsh, Alex J, Skala, Melissa C, Moses, Harold L, Merchant, Nipun B

“…Background & Aims A hallmark of pancreatic ductal adenocarcinoma (PDAC) is the presence of a dense desmoplastic reaction (stroma) that impedes drug delivery to…”
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Optical metabolic imaging of treatment response in human head and neck squamous cell carcinoma by Shah, Amy T, Demory Beckler, Michelle, Walsh, Alex J, Jones, William P, Pohlmann, Paula R, Skala, Melissa C

“…Optical metabolic imaging measures fluorescence intensity and lifetimes from metabolic cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine…”
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Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth by Benavides, Oscar R, Gibbs, Holly C, White, Berkley P, Kaunas, Roland, Gregory, Carl A, Walsh, Alex J, Maitland, Kristen C

“…The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier…”
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Optical Imaging of Drug-Induced Metabolism Changes in Murine and Human Pancreatic Cancer Organoids Reveals Heterogeneous Drug Response by Walsh, Alex J, Castellanos, Jason A, Nagathihalli, Nagaraj S, Merchant, Nipun B, Skala, Melissa C

“…Three-dimensional organoids derived from primary pancreatic ductal adenocarcinomas are an attractive platform for testing potential anticancer drugs on…”
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Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells by Lee, Woojong, Kingstad-Bakke, Brock, Paulson, Brett, Larsen, Autumn, Overmyer, Katherine, Marinaik, Chandranaik B, Dulli, Kelly, Toy, Randall, Vogel, Gabriela, Mueller, Katherine P, Tweed, Kelsey, Walsh, Alex J, Russell, Jason, Saha, Krishanu, Reyes, Leticia, Skala, Melissa C, Sauer, John-Demian, Shayakhmetov, Dmitry M, Coon, Joshua, Roy, Krishnendu, Suresh, M

“…There is a critical need for adjuvants that can safely elicit potent and durable T cell-based immunity to intracellular pathogens. Here, we report that…”
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Identification of rare cell populations in autofluorescence lifetime image data by Cardona, Elizabeth N., Walsh, Alex J.

“…Drug‐resistant cells and anti‐inflammatory immune cells within tumor masses contribute to tumor aggression, invasion, and worse patient outcomes. These cells…”
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Fluorescence intensity and lifetime redox ratios detect metabolic perturbations in T cells by Hu, Linghao, Wang, Nianchao, Cardona, Elizabeth, Walsh, Alex J.

“…The auto-fluorescent coenzymes reduced nicotinamide dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD) allow label-free detection of cellular…”
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Functional Optical Imaging of Primary Human Tumor Organoids: Development of a Personalized Drug Screen by Walsh, Alex J, Cook, Rebecca S, Skala, Melissa C

“…Primary tumor organoids are a robust model of individual human cancers and present a unique platform for patient-specific drug testing. Optical imaging is…”
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Addressing structural hurdles for metadata extraction from environmental impact statements by Laparra, Egoitz, Binford‐Walsh, Alex, Emerson, Kirk, Miller, Marc L., López‐Hoffman, Laura, Currim, Faiz, Bethard, Steven

“…Natural language processing techniques can be used to analyze the linguistic content of a document to extract missing pieces of metadata. However, accurate…”
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Extracellular pH affects the fluorescence lifetimes of metabolic co-factors by Schmitz, Rebecca, Tweed, Kelsey, Walsh, Christine, Walsh, Alex J, Skala, Melissa C

“…Significance: Autofluorescence measurements of the metabolic cofactors NADH and flavin adenine dinucleotide (FAD) provide a label-free method to quantify…”
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Optical metabolic imaging quantifies heterogeneous cell populations by Walsh, Alex J, Skala, Melissa C

“…The genetic and phenotypic heterogeneity of cancers can contribute to tumor aggressiveness, invasion, and resistance to therapy. Fluorescence imaging occupies…”
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In Vivo Autofluorescence Imaging of Tumor Heterogeneity in Response to Treatment by Shah, Amy T, Diggins, Kirsten E, Walsh, Alex J, Irish, Jonathan M, Skala, Melissa C

“…Abstract Subpopulations of cells that escape anti-cancer treatment can cause relapse in cancer patients. Therefore, measurements of cellular-level tumor…”
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Drug response in organoids generated from frozen primary tumor tissues by Walsh, Alex J., Cook, Rebecca S., Sanders, Melinda E., Arteaga, Carlos L., Skala, Melissa C.

“…Primary tumor organoids grown in three-dimensional culture provide an excellent platform for studying tumor progression, invasion and drug response. However,…”
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Classifying T cell activity in autofluorescence intensity images with convolutional neural networks by Wang, Zijie J., Walsh, Alex J., Skala, Melissa C., Gitter, Anthony

“…The importance of T cells in immunotherapy has motivated developing technologies to improve therapeutic efficacy. One objective is assessing antigen‐induced T…”
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Comparison of polystyrene and hydrogel microcarriers for optical imaging of adherent cells by Benavides, Oscar R, White, Berkley P, Gibbs, Holly C, Kaunas, Roland, Gregory, Carl A, Maitland, Kristen C, Walsh, Alex J

“…The ability to observe and monitor cell density and morphology has been imperative for assessing the health of a cell culture and for producing high quality,…”
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Autofluorescence Imaging to Evaluate Cellular Metabolism by Theodossiou, Anna, Hu, Linghao, Wang, Nianchao, Nguyen, Uyen, Walsh, Alex J

“…Cellular metabolism is the process by which cells generate energy, and many diseases, including cancer, are characterized by abnormal metabolism. Reduced…”
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