Search Results - "Walker, G. Terrance"
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Predicting Antibiotic Resistance in Gram-Negative Bacilli from Resistance Genes
Published in Antimicrobial agents and chemotherapy (01-04-2019)“…We developed a rapid high-throughput PCR test and evaluated highly antibiotic-resistant clinical isolates of ( = 2,919), ( = 1,974), ( = 1,150), and ( =…”
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Rapid Molecular Screening for Gram-Negative Antimicrobial-Resistance Genes with Commercially Available Methods
Published in Clinical microbiology newsletter (01-11-2015)“…Abstract Antibiotic-resistant bacteria threaten the well-being of many hospitalized patients and cause an added financial burden on health care systems. There…”
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Multicenter Evaluation of the Acuitas AMR Gene Panel for Detection of an Extended Panel of Antimicrobial Resistance Genes among Bacterial Isolates
Published in Journal of clinical microbiology (16-03-2022)“…The Acuitas antimicrobial resistance (AMR) gene panel is a qualitative, multiplex, nucleic acid-based diagnostic test for the detection and differentiation of…”
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Clinical evaluation of the acuitas® AMR gene panel for rapid detection of bacteria and genotypic antibiotic resistance determinants
Published in Diagnostic microbiology and infectious disease (01-08-2021)“…Urinary tract infections are leading causes of hospital admissions. Accurate and timely diagnosis is important due to increasing morbidity and mortality from…”
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Isothermal in vitro Amplification of DNA by a Restriction Enzyme/DNA Polymerase System
Published in Proceedings of the National Academy of Sciences - PNAS (01-01-1992)“…An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent…”
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Analytical Performance of Multiplexed Screening Test for 10 Antibiotic Resistance Genes from Perianal Swab Samples
Published in Clinical chemistry (Baltimore, Md.) (01-02-2016)“…Multiantibiotic-resistant bacteria pose a threat to patients and place an economic burden on health care systems. Carbapenem-resistant bacilli and…”
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Temperature and Quenching Studies of Fluorescence Polarization Detection of DNA Hybridization
Published in Analytical chemistry (Washington) (01-02-1997)“…The effects of temperature and collisional quenching on fluorescence polarization detection of DNA hybridization were studied using measurements of…”
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Routine, Molecular, and Sequence-Based Antimicrobial Susceptibility Testing: Progression from Research Databases to Future Predictive Models
Published in Clinical microbiology newsletter (01-10-2021)“…Automated antimicrobial susceptibility testing (AST) is the standard clinical diagnostic for antibiotic resistance. The polymerase chain reaction and genome…”
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Ethidium binding to left-handed (Z) DNAs results in regions of right-handed DNA at the intercalation site
Published in Biochemistry (Easton) (01-12-1985)“…The equilibrium binding of ethidium to the right-handed (B) and left-handed (Z) forms of poly(dG-dC).poly(dG-dC) and poly(dG-m5dC).poly(dG-m5dC) was…”
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DNA Detection by Strand Displacement Amplification and Fluorescence Polarization With Signal Enhancement Using a DNA Binding Protein
Published in Nucleic acids research (15-01-1996)“…Strand displacement amplification (SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with…”
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Hybridization of fluorescein-labeled DNA oligomers detected by fluorescence anisotropy with protein binding enhancement
Published in Analytical chemistry (Washington) (01-11-1995)“…Fundamental aspects of the application of fluorescence anisotropy to detect the hybridization of fluorescein-labeled DNA oligomers were explored. The oligomers…”
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Use of the restriction enzyme AvaI and exo- Bst polymerase in strand displacement amplification
Published in BioTechniques (01-03-1998)Get full text
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A DNA probe assay using strand displacement amplification (SDA) and filtration to separate reacted and unreacted detector probes
Published in Molecular and cellular probes (01-12-1995)“…Strand displacement amplification (SDA) is an isothermal, in vitromethod of amplifying a target DNA sequence. We performed SDA in the presence of a 5-…”
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Strand displacement amplification—an isothermal, in vitro DNA amplification technique
Published in Nucleic acids research (11-04-1992)“…Strand Displacement Amplification (SDA) Is an isothermal, in vitro nucleic acid amplification technique based upon the ability of Hindi to nick the unmodified…”
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B-Z cooperativity and kinetics of poly(dG-m5dC) are controlled by an unfavorable B-Z interface energy
Published in Journal of biomolecular structure & dynamics (01-06-1988)“…Thermodynamic and kinetic properties of the B-Z transition of poly(dG-m5dC) were investigated using polynucleotide samples ranging in length from 11000 to 300…”
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Empirical aspects of strand displacement amplification
Published in Genome research (01-08-1993)Get full text
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Quantitative Reverse Transcription Strand Displacement Amplification: Quantitation of Nucleic Acids Using an Isothermal Amplification Technique
Published in Analytical biochemistry (01-06-1998)“…Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent…”
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Detection of Mycobacterium tuberculosis DNA with thermophilic strand displacement amplification and fluorescence polarization
Published in Clinical chemistry (Baltimore, Md.) (01-10-1996)“…Strand displacement amplification (SDA) is an isothermal, in vitro method for diagnostics that amplifies a target DNA sequence by using a restriction enzyme…”
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Multiplex strand displacement amplification (SDA) and detection of DNA sequences from Mycobacterium tuberculosis and other mycobacteria
Published in Nucleic acids research (11-07-1994)“…Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic…”
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Simultaneous strand displacement amplification and fluorescence polarization detection of Chlamydia trachomatis DNA
Published in Analytical biochemistry (05-04-1997)“…Strand displacement amplification (SDA) is an isothermal DNA amplification technology that uses a restriction enzyme and polymerase. We have developed a…”
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