Search Results - "Walinda, Erik"

Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy by Iwakawa, Naoto, Morimoto, Daichi, Walinda, Erik, Shirakawa, Masahiro, Sugase, Kenji

“…Formation of protein aggregates or fibrils entails the conversion of soluble native protein monomers via multiple molecular states. No spectroscopic techniques…”
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Pinpoint analysis of a protein in slow exchange using F1F2-selective ZZ-exchange spectroscopy: assignment and kinetic analysis by Nishizawa, Mayu, Walinda, Erik, Morimoto, Daichi, Sugase, Kenji

“…ZZ-exchange spectroscopy is widely used to study slow exchange processes in biomolecules, especially determination of exchange rates and assignment of minor…”
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Expression, solubility monitoring, and purification of the co-folded LUBAC LTM domain by structure-guided tandem folding in autoinducing cultures by Walinda, Erik, Morimoto, Daichi, Sorada, Tomoki, Iwai, Kazuhiro, Sugase, Kenji

“…The linear ubiquitin chain assembly complex tethering motif (LUBAC-LTM) domain is composed of two different accessory LUBAC components (HOIL-1L and SHARPIN)…”
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Structural Dynamic Heterogeneity of Polyubiquitin Subunits Affects Phosphorylation Susceptibility by Morimoto, Daichi, Walinda, Erik, Takashima, Shingo, Nishizawa, Mayu, Iwai, Kazuhiro, Shirakawa, Masahiro, Sugase, Kenji

“…Polyubiquitin is a multifunctional protein tag formed by the covalent conjugation of ubiquitin molecules. Due to the high rigidity of the ubiquitin fold, the…”
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Visualizing protein motion in Couette flow by all-atom molecular dynamics by Walinda, Erik, Morimoto, Daichi, Shirakawa, Masahiro, Scheler, Ulrich, Sugase, Kenji

“…In living cells, biomacromolecules are exposed to a highly crowded environment. The cytoplasm, the nucleus, and other organelles are highly viscous fluids that…”
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Quantitative monitoring of ubiquitination/deubiquitination reaction cycles by 18O-incorporation by Tanaka, Yuka, Morimoto, Daichi, Walinda, Erik, Sugase, Kenji, Shirakawa, Masahiro

“…Ubiquitination is one of the major post-translational modifications and entails conjugation of ubiquitin molecules to target proteins. To make free ubiquitin…”
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Dual Function of Phosphoubiquitin in E3 Activation of Parkin by Walinda, Erik, Morimoto, Daichi, Sugase, Kenji, Shirakawa, Masahiro

“…Mutations in the gene encoding parkin, an auto-inhibited E3 ubiquitin ligase that functions in the clearance of damaged mitochondria, are the most common cause…”
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Cooperative Domain Formation by Homologous Motifs in HOIL-1L and SHARPIN Plays A Crucial Role in LUBAC Stabilization by Fujita, Hiroaki, Tokunaga, Akira, Shimizu, Satoshi, Whiting, Amanda L., Aguilar-Alonso, Francisco, Takagi, Kenji, Walinda, Erik, Sasaki, Yoshiteru, Shimokawa, Taketo, Mizushima, Tsunehiro, Ohki, Izuru, Ariyoshi, Mariko, Tochio, Hidehito, Bernal, Federico, Shirakawa, Masahiro, Iwai, Kazuhiro

“…The linear ubiquitin chain assembly complex (LUBAC) participates in inflammatory and oncogenic signaling by conjugating linear ubiquitin chains to target…”
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Ubiquitylation Directly Induces Fold Destabilization of Proteins by Morimoto, Daichi, Walinda, Erik, Fukada, Harumi, Sugase, Kenji, Shirakawa, Masahiro

“…Ubiquitin is a common post-translational modifier and its conjugation is a key signal for proteolysis by the proteasome. Because the molecular mass of…”
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Isolation and characterization of a minimal building block of polyubiquitin fibrils by Morimoto, Daichi, Walinda, Erik, Shinke, Mayo, Sugase, Kenji, Shirakawa, Masahiro

“…As a posttranslational modifier, polyubiquitin is involved in the regulation of diverse intracellular processes; however, it is also found in pathological…”
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Backbone resonance assignments of the A2 domain of mouse von Willebrand factor by Morimoto, Daichi, Osugi, Masanori, Mahana, Yutaka, Walinda, Erik, Shirakawa, Masahiro, Sugase, Kenji

“…von Willebrand factor (vWF) is an adhesive plasma protein that is important for platelet adhesion in normal hemostasis in response to vascular injury. Although…”
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Overview of Relaxation Dispersion NMR Spectroscopy to Study Protein Dynamics and Protein-Ligand Interactions by Walinda, Erik, Morimoto, Daichi, Sugase, Kenji

“…Proteins and nucleic acids are central to all biological processes. NMR spectroscopy has proven to be excellent for studying the dynamics of these…”
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Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR by Iwakawa, Naoto, Morimoto, Daichi, Walinda, Erik, Kawata, Yasushi, Shirakawa, Masahiro, Sugase, Kenji

“…Amyloid fibril formation is associated with numerous neurodegenerative diseases. To elucidate the mechanism of fibril formation, the thioflavin T (ThT)…”
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Biological and Physicochemical Functions of Ubiquitylation Revealed by Synthetic Chemistry Approaches by Morimoto, Daichi, Walinda, Erik, Sugase, Kenji, Shirakawa, Masahiro

“…Most intracellular proteins are subjected to post-translational modification by ubiquitin. Accordingly, it is of fundamental importance to investigate the…”
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Structural Insights into Methylated DNA Recognition by the Methyl-CpG Binding Domain of MBD6 from Arabidopsis thaliana by Mahana, Yutaka, Ohki, Izuru, Walinda, Erik, Morimoto, Daichi, Sugase, Kenji, Shirakawa, Masahiro

“…Cytosine methylation is an epigenetic modification essential for formation of mature heterochromatin, gene silencing, and genomic stability. In plants,…”
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NMR resonance assignments of the NZF domain of mouse HOIL-1L free and bound to linear di-ubiquitin by Ishii, Naoki, Walinda, Erik, Iwakawa, Naoto, Morimoto, Daichi, Iwai, Kazuhiro, Sugase, Kenji, Shirakawa, Masahiro

“…Nuclear factor-κB (NF-κB) activation plays a central role in immunity and inflammation. In the canonical NF-κB activation pathway, linear polyubiquitin chains…”
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Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils by Morimoto, Daichi, Nishizawa, Ryo, Walinda, Erik, Takashima, Shingo, Sugase, Kenji, Shirakawa, Masahiro

“…Ubiquitin and its polymeric forms are conjugated to intracellular proteins to regulate diverse intracellular processes. Intriguingly, polyubiquitin has also…”
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Backbone resonance assignments of monomeric SOD1 in dilute and crowded environments by Iwakawa, Naoto, Morimoto, Daichi, Walinda, Erik, Sugase, Kenji, Shirakawa, Masahiro

“…Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that leads to movement disorders. In motor neurons of ALS patients, intracellular…”
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Backbone and side-chain resonance assignments of the methyl-CpG-binding domain of MBD6 from Arabidopsis thaliana by Iwakawa, Naoto, Mahana, Yutaka, Ono, Arina, Ohki, Izuru, Walinda, Erik, Morimoto, Daichi, Sugase, Kenji, Shirakawa, Masahiro

“…Epigenetic regulation is essential to various biological phenomena such as cell differentiation and cancer. DNA methylation is one of the most important…”
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A fast and simple automated multi-step protein purification method for ÄKTA go systems by Morimoto, Daichi, Walinda, Erik

“…Automation of protein purification methods can increase researchers' efficiency in life sciences. However, currently reported automated protein purification…”
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