Search Results - "Waldo, Gary L"

Agonist binding and Gq-stimulating activities of the purified human P2Y1 receptor by Waldo, Gary L, Harden, T Kendall

“…The human P2Y1 receptor (P2Y1-R) was purified after high-level expression from a recombinant baculovirus in Sf9 insect cells. Quantification by protein…”
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RGS6, RGS7, RGS9, and RGS11 Stimulate GTPase Activity of Gi Family G-proteins with Differential Selectivity and Maximal Activity by Hooks, Shelley B, Waldo, Gary L, Corbitt, James, Bodor, Erik T, Krumins, Andrejs M, Harden, T Kendall

“…Regulator of G-protein signaling (RGS) proteins are GTPase activating proteins (GAPs) of heterotrimeric G-proteins that alter the amplitude and kinetics of…”
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Purification and functional reconstitution of the human P2Y12 receptor by Bodor, Erik T, Waldo, Gary L, Hooks, Shelley B, Corbitt, James, Boyer, Jose L, Harden, T Kendall

“…The human P2Y12 receptor (P2Y12-R) is a member of the G protein coupled P2Y receptor family, which is intimately involved in platelet physiology. We describe…”
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Kinetic Scaffolding Mediated by a Phospholipase C-β and Gq Signaling Complex by Waldo, Gary L, Ricks, Tiffany K, Hicks, Stephanie N, Cheever, Matthew L, Kawano, Takeharu, Tsuboi, Kazuhito, Wang, Xiaoyue, Montell, Craig, Kozasa, Tohru, Sondek, John, Harden, T. Kendall

“…Transmembrane signals initiated by a broad range of extracellular stimuli converge on nodes that regulate phospholipase C (PLC)-dependent inositol lipid…”
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Membrane-induced Allosteric Control of Phospholipase C-β Isozymes by Charpentier, Thomas H., Waldo, Gary L., Barrett, Matthew O., Huang, Weigang, Zhang, Qisheng, Harden, T. Kendall, Sondek, John

“…All peripheral membrane proteins must negotiate unique constraints intrinsic to the biological interface of lipid bilayers and the cytosol. Phospholipase C-β…”
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Potent and Selective Peptide-based Inhibition of the G Protein Gαq by Charpentier, Thomas H., Waldo, Gary L., Lowery-Gionta, Emily G., Krajewski, Krzysztof, Strahl, Brian D., Kash, Thomas L., Harden, T. Kendall, Sondek, John

“…In contrast to G protein-coupled receptors, for which chemical and peptidic inhibitors have been extensively explored, few compounds are available that…”
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Mechanism of Activation and Inactivation of Gq/Phospholipase C-β Signaling Nodes by Harden, T. Kendall, Waldo, Gary L, Hicks, Stephanie N, Sondek, John

“…Harden et al examine C-beta signaling nodes with regard to the mechanism of activation and inactivation of Gq/phospholipase…”
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G protein-coupled receptor-mediated activation of p110β by Gβγ is required for cellular transformation and invasiveness by Dbouk, Hashem A, Vadas, Oscar, Shymanets, Aliaksei, Burke, John E, Salamon, Rachel S, Khalil, Bassem D, Barrett, Mathew O, Waldo, Gary L, Surve, Chinmay, Hsueh, Christine, Perisic, Olga, Harteneck, Christian, Shepherd, Peter R, Harden, T Kendall, Smrcka, Alan V, Taussig, Ronald, Bresnick, Anne R, Nürnberg, Bernd, Williams, Roger L, Backer, Jonathan M

“…Synergistic activation by heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) and receptor tyrosine kinases distinguishes…”
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A unique fold of phospholipase C-β mediates dimerization and interaction with Gαq by Sondek, John, Singer, Alex U, Waldo, Gary L, Harden, T. Kendall

“…GTP-bound subunits of the Gq family of Gα subunits directly activate phospholipase C-β (PLC-β) isozymes to produce the second messengers inositol…”
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Delineation of ligand binding and receptor signaling activities of purified P2Y receptors reconstituted with heterotrimeric G proteins by Bodor, Erik T, Waldo, Gary L, Blaesius, Rainer, Harden, T Kendall

“…P2Y receptors are G protein coupled receptors that respond to extracellular nucleotides to promote a multitude of signaling events. Our laboratory has purified…”
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Molecular cloning and expression of an avian G protein-coupled P2Y receptor by Boyer, J L, Waldo, G L, Harden, T K

“…A family of G protein-coupled P2Y receptors that are activated by adenine and uridine nucleotides has been identified recently. Degenerate primers based on…”
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Protein Kinase C Phosphorylates RGS2 and Modulates Its Capacity for Negative Regulation of Gα11 Signaling by Cunningham, Michelle L., Waldo, Gary L., Hollinger, Susanne, Hepler, John R., Harden, T.Kendall

“…RGS proteins (regulators of G protein signaling) attenuate heterotrimeric G protein signaling by functioning as both GTPase-activating proteins (GAPs) and…”
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Purification of phospholipase C-activating G protein, G11, from turkey erythrocytes by Waldo, G L, Boyer, J L, Harden, T K

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Kinetic Scaffolding Mediated by a Phospholipase C–β and G q Signaling Complex by Waldo, Gary L., Ricks, Tiffany K., Hicks, Stephanie N., Cheever, Matthew L., Kawano, Takeharu, Tsuboi, Kazuhito, Wang, Xiaoyue, Montell, Craig, Kozasa, Tohru, Sondek, John, Harden, T. Kendall

“…Reciprocal Regulation An essential step in many signaling cascades is inositol lipid hydrolysis catalyzed by phospholipase C–β. The latter is activated by the…”
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Kinetic Scaffolding Mediated by a Phospholipase C-β and GqSignaling Complex by Waldo, Gary L., Ricks, Tiffany K., Hicks, Stephanie N., Cheever, Matthew L., Kawano, Takeharu, Tsuboi, Kazuhito, Wang, Xiaoyue, Montell, Craig, Kozasa, Tohru, Sondek, John, Harden, T. Kendall

“…Transmembrane signals initiated by a broad range of extracellular stimuli converge on nodes that regulate phospholipase (PLC)-dependent inositol lipid…”
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Kinetic Scaffolding Mediated by a Phospholipase C-[beta] and Gq Signaling Complex by Waldo, Gary L, Ricks, Tiffany K, Hicks, Stephanie N, Cheever, Matthew L, Kawano, Takeharu, Tsuboi, Kazuhito, Wang, Xiaoyue, Montell, Craig, Kozasa, Tohru, Sondek, John, T. Kendall Harden

“…An essential step in many signaling cascades is inositol lipid hydrolysis catalyzed by phospholipase C-β. The latter is activated by the α subunit of the…”
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Quantitation of the P2Y(1) receptor with a high affinity radiolabeled antagonist by Waldo, Gary L, Corbitt, James, Boyer, José L, Ravi, Gnana, Kim, Hak Sung, Ji, Xiao-Duo, Lacy, James, Jacobson, Kenneth A, Harden, T Kendall

“…2-Chloro-N(6)-methyl-(N )-methanocarba-2'-deoxyadenosine-3',5'- bisphosphate (MRS2279) was developed previously as a selective high-affinity, non-nucleotide…”
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βγ-Subunit activation of G-protein-regulated phospholipase C by BOYER, J. L, WALDO, G. L, HARDEN, T. K

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Selective activation of phospholipase C by recombinant G-protein α- and βγ-subunits by BOYER, J. L, GRABER, S. G, WALDO, G. L, HARDEN, T. K, GARRISON, J. C

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Protein Kinase C Phosphorylates RGS2 and Modulates Its Capacity for Negative Regulation of Gα11 Signaling by Michelle L. Cunningham, Gary L. Waldo, Susanne Hollinger, John R. Hepler, T. Kendall Harden

“…RGS proteins (regulators of G protein signaling) attenuate heterotrimeric G protein signaling by functioning as both GTPase-activating proteins (GAPs) and…”
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