Search Results - "Wagner, E. Gerhart H."

An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli by Romilly, Cédric, Lippegaus, Anne, Wagner, E Gerhart H

“…Abstract In response to DNA damage, Escherichia coli cells activate the expression of the toxin gene tisB of the toxin–antitoxin system tisB-istR1. Of three…”
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Two regulatory RNA elements affect TisB‐dependent depolarization and persister formation by Berghoff, Bork A., Hoekzema, Mirthe, Aulbach, Lena, Wagner, E. Gerhart H.

“…Summary Bacterial survival strategies involve phenotypic diversity which is generated by regulatory factors and noisy expression of effector proteins. The…”
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Unstructured 5′-tails act through ribosome standby to override inhibitory structure at ribosome binding sites by Sterk, Maaike, Romilly, Cédric, Wagner, E Gerhart H

“…Abstract Initiation is the rate-limiting step in translation. It is well-known that stable structure at a ribosome binding site (RBS) impedes initiation. The…”
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The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella by Rizvanovic, Alisa, Michaux, Charlotte, Panza, Margherita, Iloglu, Zeynep, Helaine, Sophie, Wagner, E Gerhart H, Holmqvist, Erik

“…Bacterial populations can survive exposure to antibiotics through transient phenotypic and gene expression changes. These changes can be attributed to a small…”
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RNA-based regulation in type I toxin–antitoxin systems and its implication for bacterial persistence by Berghoff, Bork A., Wagner, E. Gerhart H.

“…Bacterial dormancy is a valuable survival strategy upon challenging environmental conditions. Dormant cells tolerate the consequences of high stress levels and…”
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RNAs actively cycle on the Sm-like protein Hfq by Fender, Aurélie, Elf, Johan, Hampel, Kornelia, Zimmermann, Bastian, Wagner, E Gerhart H

“…Hfq, a protein required for small RNA (sRNA)-mediated regulation in bacteria, binds RNA with low-nanomolar K(d) values and long half-lives of complexes (>100…”
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small SOS-induced toxin is targeted against the inner membrane in Escherichia coli by Unoson, Cecilia, Wagner, E. Gerhart H

“…We previously reported on an SOS-induced toxin, TisB, in Escherichia coli and its regulation by the RNA antitoxin IstR-1. Here, we addressed the mode of action…”
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Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms by Timmusk, Salme, Grantcharova, Nina, Wagner, E. Gerhart H

“…Paenibacillus polymyxa is a plant growth-promoting rhizobacterium with a broad host range, but so far the use of this organism as a biocontrol agent has not…”
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A nascent polypeptide sequence modulates DnaA translation elongation in response to nutrient availability by Felletti, Michele, Romilly, Cédric, Wagner, E Gerhart H, Jonas, Kristina

“…The ability to regulate DNA replication initiation in response to changing nutrient conditions is an important feature of most cell types. In bacteria, DNA…”
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A mixed double negative feedback loop between the sRNA MicF and the global regulator Lrp by Holmqvist, Erik, Unoson, Cecilia, Reimegård, Johan, Wagner, E. Gerhart H.

“…Summary Roughly 10% of all genes in Escherichia coli are controlled by the global transcription factor Lrp, which responds to nutrient availability…”
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H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO by Westra, Edze R, Pul, Ümit, Heidrich, Nadja, Jore, Matthijs M, Lundgren, Magnus, Stratmann, Thomas, Wurm, Reinhild, Raine, Amanda, Mescher, Melina, van Heereveld, Luc, Mastop, Marieke, Wagner, E. Gerhart H, Schnetz, Karin, van der Oost, John, Wagner, Rolf, Brouns, Stan J.J

“…The recently discovered prokaryotic CRISPR/Cas defence system provides immunity against viral infections and plasmid conjugation. It has been demonstrated that…”
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Target identification of small noncoding RNAs in bacteria by Vogel, Jörg, Wagner, E Gerhart H

“…Small noncoding RNAs have been discovered at a staggering rate in Escherichia coli and many other bacteria. Most of the sRNAs of known function regulate gene…”
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The toxin-antitoxin system tisB-istR1: Expression, regulation, and biological role in persister phenotypes by Wagner, E. Gerhart H., Unoson, Cecilia

“…Chromosomally encoded toxin-antitoxin (TA) systems are abundantly present in bacteria and archaea. They have become a hot topic in recent years, because-after…”
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Hfq‐dependent mRNA unfolding promotes sRNA‐based inhibition of translation by Hoekzema, Mirthe, Romilly, Cédric, Holmqvist, Erik, Wagner, E Gerhart H

“…Small RNAs post‐transcriptionally regulate many processes in bacteria. Base‐pairing of sRNAs near ribosome‐binding sites in mRNAs inhibits translation, often…”
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Sigma E controls biogenesis of the antisense RNA MicA by Udekwu, Klas I, Wagner, E. Gerhart H

“…Adaptation stress responses in the Gram-negative bacterium Escherichia coli and its relatives involve a growing list of small regulatory RNAs (sRNAs). Previous…”
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Kill the messenger: bacterial antisense RNA promotes mRNA decay by Wagner, E Gerhart H

“…Bacterial antisense RNAs target translation initiation regions (TIRs) to compete with ribosome binding, thus repressing translation and—secondarily—causing…”
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RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study by Berghoff, Bork A, Karlsson, Torgny, Källman, Thomas, Wagner, E Gerhart H, Grabherr, Manfred G

“…Measuring how gene expression changes in the course of an experiment assesses how an organism responds on a molecular level. Sequencing of RNA molecules, and…”
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The ribosomal protein S1-dependent standby site in tisB mRNA consists of a single-stranded region and a 5′ structure element by Romilly, Cédric, Deindl, Sebastian, Wagner, E. Gerhart H.

“…In bacteria, stable RNA structures that sequester ribosome-binding sites (RBS) impair translation initiation, and thus protein output. In some cases, ribosome…”
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Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA by Djupedal, Ingela, Kos-Braun, Isabelle C, Mosher, Rebecca A, Söderholm, Niklas, Simmer, Femke, Hardcastle, Thomas J, Fender, Aurélie, Heidrich, Nadja, Kagansky, Alexander, Bayne, Elizabeth, Wagner, E Gerhart H, Baulcombe, David C, Allshire, Robin C, Ekwall, Karl

“…The formation of heterochromatin at the centromeres in fission yeast depends on transcription of the outer repeats. These transcripts are processed into siRNAs…”
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An Antisense RNA Inhibits Translation by Competing with Standby Ribosomes by Darfeuille, Fabien, Unoson, Cecilia, Vogel, Jörg, Wagner, E. Gerhart H.

“…Most antisense RNAs in bacteria inhibit translation by competing with ribosomes for translation initiation regions (TIRs) on nascent mRNA. We propose a…”
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