Search Results - "Waghmare, Sakharam"

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions by Wiedenheft, Blake, van Duijn, Esther, Bultema, Jelle, Waghmare, Sakharam, Zhou, Kaihong, Barendregt, Arjan, Westphal, Wiebke, Heck, Albert, Boekema, Egbert, Dickman, Mark, Doudna, Jennifer A

“…Prokaryotes have evolved multiple versions of an RNA-guided adaptive immune system that targets foreign nucleic acids. In each case, transcripts derived from…”
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In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus by Sinkunas, Tomas, Gasiunas, Giedrius, Waghmare, Sakharam P, Dickman, Mark J, Barrangou, Rodolphe, Horvath, Philippe, Siksnys, Virginijus

“…Clustered regularly interspaced short palindromic repeats (CRISPR)‐encoded immunity in Type I systems relies on the Cascade (CRISPR‐associated complex for…”
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SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis by Baena, Guillermo, Xia, Lingfeng, Waghmare, Sakharam, Karnik, Rucha

“…The vesicle trafficking SYNTAXIN OF PLANTS132 (SYP132) drives hormone-regulated endocytic traffic to suppress the density and function of plasma membrane (PM)…”
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Binding of SEC11 Indicates Its Role in SNARE Recycling after Vesicle Fusion and Identifies Two Pathways for Vesicular Traffic to the Plasma Membrane by Karnik, Rucha, Zhang, Ben, Waghmare, Sakharam, Aderhold, Christin, Grefen, Christopher, Blatt, Michael R.

“…SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion in all eukaryotes and contribute to homeostasis,…”
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Characterization and Quantification of RNA Post-transcriptional Modifications Using Stable Isotope Labeling of RNA in Conjunction with Mass Spectrometry Analysis by Waghmare, Sakharam P, Dickman, Mark J

“…Mass spectrometry has emerged as an increasingly powerful tool for the identification and characterization of nucleic acids, in particular RNA…”
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Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS) by Waghmare, Sakharam P, Nwokeoji, Alison O, Dickman, Mark J

“…Mass spectrometry is a powerful tool for characterizing RNA. Here we describe a method for the identification and characterisation of crRNA using liquid…”
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A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion by Grefen, Christopher, Karnik, Rucha, Larson, Emily, Lefoulon, Cécile, Wang, Yizhou, Waghmare, Sakharam, Zhang, Ben, Hills, Adrian, Blatt, Michael R.

“…Growth in plants depends on ion transport for osmotic solute uptake and secretory membrane trafficking to deliver material for wall remodelling and cell…”
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Studying the mechanism of RNA separations using RNA chromatography and its application in the analysis of ribosomal RNA and RNA:RNA interactions by Waghmare, Sakharam P., Pousinis, Petros, Hornby, David P., Dickman, Mark J.

“…DNA/RNA chromatography presents a versatile platform for the analysis of nucleic acids. Although the mechanism of separation of double stranded (ds) DNA…”
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Structural basis for CRISPR RNA-guided DNA recognition by Cascade by Jore, Matthijs M, Lundgren, Magnus, van Duijn, Esther, Bultema, Jelle B, Westra, Edze R, Waghmare, Sakharam P, Wiedenheft, Blake, Pul, Ümit, Wurm, Reinhild, Wagner, Rolf, Beijer, Marieke R, Barendregt, Arjan, Zhou, Kaihong, Snijders, Ambrosius P L, Dickman, Mark J, Doudna, Jennifer A, Boekema, Egbert J, Heck, Albert J R, van der Oost, John, Brouns, Stan J J

“…The CRISPR system is a prokaryotic immune system that depends on the Cascade protein complex and small guide RNAs (crRNAs). The Cascade complex, loaded with…”
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SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets by Waghmare, Sakharam, Lileikyte, Edita, Karnik, Rucha, Goodman, Jennifer K., Blatt, Michael R., Jones, Alexandra M.E.

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K + Channel-SEC11 Binding Exchange Regulates SNARE Assembly for Secretory Traffic by Waghmare, Sakharam, Lefoulon, Cecile, Zhang, Ben, Liliekyte, Edita, Donald, Naomi, Blatt, Michael R

“…Cell expansion requires that ion transport and secretory membrane traffic operate in concert. Evidence from Arabidopsis ( ) indicates that such coordination is…”
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Gating control and K+ uptake by the KAT1 K+ channel leaveraged through membrane anchoring of the trafficking protein SYP121 by Lefoulon, Cécile, Waghmare, Sakharam, Karnik, Rucha, Blatt, Michael R.

“…Vesicle traffic is tightly coordinated with ion transport for plant cell expansion through physical interactions between subsets of vesicle‐trafficking…”
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SYNTAXIN OF PLANTS 132 underpins secretion of cargoes associated with salicylic acid signaling and pathogen defense by Waghmare, Sakharam, Xia, Lingfeng, Ly, Thu Phan, Xu, Jing, Farami, Sahar, Burchmore, Richard, Blatt, Michael R, Karnik, Rucha

“…Secretory trafficking in plant cells is facilitated by SNARE (soluble N-ethylamide-sensitive factor attachment protein receptor) proteins that drive membrane…”
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Arabidopsis SNARE SYP132 impacts on PIP2;1 trafficking and function in salinity stress by Baena, Guillermo, Xia, Lingfeng, Waghmare, Sakharam, Yu, ZhiYi, Guo, Yue, Blatt, Michael R., Zhang, Ben, Karnik, Rucha

“…SUMMARY In plants so‐called plasma membrane intrinsic proteins (PIPs) are major water channels governing plant water status. Membrane trafficking contributes…”
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Commandeering Channel Voltage Sensors for Secretion, Cell Turgor, and Volume Control by Karnik, Rucha, Waghmare, Sakharam, Zhang, Ben, Larson, Emily, Lefoulon, Cécile, Gonzalez, Wendy, Blatt, Michael R.

“…Control of cell volume and osmolarity is central to cellular homeostasis in all eukaryotes. It lies at the heart of the century-old problem of how plants…”
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SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets by Waghmare, Sakharam, Lileikyte, Edita, Karnik, Rucha, Goodman, Jennifer K., Blatt, Michael R., Jones, Alexandra M.E.

“…SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion and contribute to homoeostasis, pathogen defense,…”
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VAMP721 Conformations Unmask an Extended Motif for K⁺ Channel Binding and Gating Control by Zhang, Ben, Karnik, Rucha, Waghmare, Sakharam, Donald, Naomi, Blatt, Michael R.

“…Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins play a major role in membrane fusion and contribute to cell expansion,…”
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K+ Channel-SEC11 Binding Exchange Regulates SNARE Assembly for Secretory Traffic1 by Waghmare, Sakharam, Lefoulon, Cecile, Zhang, Ben, Liliekyte, Edita, Donald, Naomi, Blatt, Michael R.

“…K + channels initiate a binding exchange with the SNARE SYP121 to trigger SNARE complex assembly and drive secretory vesicle fusion at the plant plasma…”
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SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets1[OPEN] by Waghmare, Sakharam, Lileikyte, Edita, Karnik, Rucha, Goodman, Jennifer K., Blatt, Michael R., Jones, Alexandra M.E.

“…Two closely related SNAREs mediate secretion of specific cargo proteins in Arabidopsis thaliana. SNARE (soluble N -ethylmaleimide-sensitive factor attachment…”
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VAMP721 Conformations Unmask an Extended Motif for K+ Channel Binding and Gating Control1[CC-BY] by Zhang, Ben, Karnik, Rucha, Waghmare, Sakharam, Donald, Naomi, Blatt, Michael R.

“…Channel binding depends on coordination between the two binding sites on VAMP721, consistent with the K + channels as nucleation points for SNARE complex…”
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